Cryopreservation of pelargonium shoot tips by encapsulation-dehydration: Effects of sucrose concentration, dehydration duration and genotype

Acta Horticulturae

Volumn 623, Issue , 2003, Pages 225-230

  Grapin, A ^a   Dumet, D ^a   Holota, H ^a   Dorion, N ^a  

^a Institut National d'Horticulture   (France)

Author keywords

Apex; Germplasm conservation; Pelargonium hortorum; Pelargonium peltatum; Regeneration

Indexed keywords

EID: 17644405346     PISSN: 05677572     EISSN: None     Source Type: Book Series    
DOI: 10.17660/ActaHortic.2003.623.24     Document Type: Conference Paper

References (13)

1. Bouafia, S., Lairy, G., Blanc, A., Bonnel, E. and Dereuddre, J. 1995 . Effets d'une préculture sur la tolérance à la congélation dans l'azote liquide des apex axillaires de vitroplants de Solanacées tubérifères (Solanum phureja et S. tuberosum). Etude après enrobage et déshydratation. Acta Bot. Gallica 142:393-402.
2. Engelmann, F. 2000. Importance of cryopreservation for the conservation of plant genetic resources. Cryopreservation of tropical plant germplasm. IPGRI, Rome: 8-20.
3. Fabre, J. and Dereuddre, J. 1990. Encapsulation-dehydration: a new approach to cryopreservationof Solanum shoot-tips. Cryo-Letters 11:413-426.
4. Escobar, R.H., Mafla, G. and Roca, W.M. 1997. A methodology for recovering cassava plants from shoot tips maintained in liquid nitrogen. Plant Cell Reports 16:474-478.
5. Grapin, A., Hupel, C., Marche, C. and Dorion, N. 2001. First results of Pelargonium shoot apex cryopreservation. Acta Hort. 560:157-160.
6. Lambardi, M., Fabbri, A. and Caccavale, A. 2000. Cryopreservation of white poplar (Populus alba L.) by vitrification of in vitro-grown shoot tips. Plant Cell Reports 19:213-218.
7. Murashige, T. and Skoog, F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497.
8. Morel, G. and Wetmore, R.H., 1951. Fern callus tissue culture. Ann. J. Bot. 38:141-143.
9. Paul, H., Daigny, G. and Sangwann-Noreel, B.S. 2000. Cryopreservation of apple (Malus x domestica Borhk.) shoot tips following encapsulation-dehydration or encapsulationvitrification. Plant Cell Reports 19:768-774.
10. Reed, B.M. and Yu, X. 1995. Cryopreservation of in vitro-grown gooseberry and currant meristems. Cryo-Letters 16:131-136.
11. Schäfer-Menuhr, A., Scumacher, H.M. and Mix-Wagner, G. 1997. Cryopreservation of potato cultivars - Design of a method for routine application in genebanks. Acta Hort. 447:477-482.
12. Wang, Q., Tanne, E., Arav, A. and Gafny, R. 2000. Cryopreservation of in vitro-grown shoot tips of grapevine by encapsulation-dehydration. Plant Cell, Tissue and Organ Culture 63:41-46.
13. Wang, Q.C., Batuman, Ö., Li, P., Bar-Joseph, M. and Gafny, R. 2002. Cryopreservation of in vitro-grown shoot tips of 'Troyer' citrange (Poncirus trifoliata (L.) Raf. x Citrus sinensis (L.) Osbeck.) by encapsulation- dehydration. Plant Cell Reports 20:901-906.