Increased hGM-CSF production and secretion with Pluronic F-68 in transgenic Nicotiana tabacum suspension cell cultures

Biotechnology and Bioprocess Engineering

Volumn 12, Issue 6, 2007, Pages 594-600

  Cho, Jong Moon ^a   Kwon, Jun Young ^a   Lim, Jung Ae ^a   Kim, Dong Il ^a  

^a Inha University   (South Korea)

Author keywords

hGM CSF; Permeabilization; Pluronic F 68; Secretion; Transgenic plant cell culture

Indexed keywords

CYTOKINE; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; NONIONIC SURFACTANT; POLOXAMER;

ARTICLE; BIOREACTOR; CELL GROWTH; CELL MEMBRANE PERMEABILITY; CELL SURVIVAL; CELL WALL; CONCENTRATION RESPONSE; CYTOKINE PRODUCTION; CYTOKINE RELEASE; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; NONHUMAN; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; SUSPENSION CELL CULTURE; TOBACCO; TRANSGENIC PLANT;

NICOTIANA TABACUM;

EID: 38349030364     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02931074     Document Type: Article

References (26)

1. Qu, J., W. Zhang, X. Yu, and M. Jin (2005) Instability of anthocyanin accumulation in Vitis vinifera L. var. Gamay Fréaux suspension cultur es. Biotechnol. Bioprocess Eng. 10: 155-161.
2. Wang, W., Z.-J. Zhao, Y. Xu, X. Qian, and J.-J. Zhong (2005) Efficient elicitation of ginsenoside biosynthesis in cell cultures of Panax notoginseng by using self-chemically-synthesized jasmonates. Biotechnol. Bioprocess Eng. 10: 162-165.
3. Choi, J.-H., S. K. Yoon, K. H. Lee, M. S. Seo, D. H. Kim, S. B. Hong, J.-Y. Kim, H.-D. Paik, and C. H. Kim (2006) Antitumor activity of cell suspension culture of green tea seed (Camellia sinensis L.). Biotechnol. Bioprocess Eng. 11: 396-401.
4. Joshi, L. and L. C. Lopez (2005) Bioprospecting in plants for engineered proteins. Curr. Opin. Plant Biol. 8: 223-226.
5. Gomord, V., P. Chamberlain, R. Jefferis, and L. Faye (2005) Biopharmaceutical production in plants: problems, solutions and opportunities. Trends Biotechnol. 23: 559-565.
6. Ma, J. K-C., R. Chikwamba, P. Sparrow, R. Fischer, R. Mahoney, and R. M. Twyman (2005) Plant-derived pharmaceuticals - the road forward. Trends Plant Sci. 10: 580-585.
7. Ma, J. K-C., E. Barros, R. Bock, P. Christou, P. J. Dale, P. J. Dix, R. Fischer, J. Irwin, R. Mahoney, M. Pezzotti, S. Schillberg, P. Sparrow, E. Stoger, and R. M. Twyman (2005) Molecular farming for new drugs and vaccines. EMBO Rep. 6: 593-599.
8. Hellwig, S., J. Drossard, R. M. Twyman, and R. Fischer (2004) Plant cell cultures for the production of recombinant proteins. Nat. Biotechnol. 22: 1415-1422.
9. Kumar, G. B. S., T. R. Ganapathi, L. Srinivas, C. J. Revathi, and V. A. Bapat (2005) Secretion of hepatitis B surface antigen in transformed tobacco cell suspension cultures. Biotechnol. Lett. 27: 927-932.
10. Chen, T.-L., Y.-L. Lin, Y.-L. Lee, N.-S. Yang, and M.-T. Chan (2004) Expression of bioactive human interferon-gamma in transgenic rice cell suspension cultures. Transgenic Res. 13:499-510.
11. Sharma, N., S.-M. Park, T.-H. Kwon, D.-H. Kim, and M.-S. Yang (2004) A simple purification procedure of biologically active recombinant human granulocyte macrophage colony stimulating factor (hGM-CSF) secreted in rice cell suspension culture. Biotechnol. Bioprocess Eng. 9: 423-427.
12. Jo, S.-H., S.-Y. Kwon, D.-S. Park, K.-S. Yang, J.-W. Kim, K.-T. Lee, S.-S. Kwak, and H.-S. Lee (2006) High-yield production of functional human lactoferrin in transgenic cell cultures of Siberian ginseng (Acanthopanax senticosus). Biotechnol. Bioprocess Eng. 11: 442-448.
13. Sharma, N., T.-G. Kim, and M.-S. Yang (2006) Production and secretion of human interleukin-18 in transgenic tobacco cell suspension culture. Biotechnol. Bioprocess Eng. 11: 154-159.
14. Metcalf, D. (1985) The granulocyte-macrophage colony-stimulating factors. Science 229:16-22.
15. Libby, R. T., G. Braedt, S. R. Kronheim, C. J. March, D. L. Urdal, T. A. Chiaverotti, R. J. Tushinski, D. Y. Mochizuki, T. P. Hopp, and D. Cosman (1987) Expression and purification of native human granulocyte-macrophage colony-stimulating factor from an Escherichia coli secretion vector. DNA 6: 221-229.
16. Huang, Y. L., C. H. Shu, and S. T. Yang (1997) Kinetics and modeling of GM-CSF production by recombinant yeast in a three-phase fluidized bed bioreactor. Biotechnol. Bioeng. 53: 470-477.
17. Caldwell, J., B. Locey, M. F. Clarke, S. G. Emerson, and B. O. Palsson (1991) Influence of medium exchange schedules on metabolic, growth, and GM-CSF secretion rates of genetically engineered NIH-3T3 cells. Biotechnol. Prog. 7: 1-8.
18. James, E. A., C. Wang, Z. Wang, R. Reeves, J. H. Shin, N. S. Magnuson, and J. M. Lee (2000) Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells. Protein Expr. Purif. 19: 131-138.
19. Shin, Y.-J., S.-Y. Hong, T.-H. Kwon, Y.-S. Jang, and M.-S. Yang (2003) High level of expression of recombinant human granulocyte-macrophage colony stimulating factor in transgenic rice cell suspension culture. Biotechnol. Bioeng. 82: 778-783.
20. Murhammer, D. W. and C. F. Goochee (1990) Sparged animal cell bioreactors: mechanism of cell damage and Pluronic F-68 protection. Biotechnol. Prog. 6: 391-397.
21. Palomares, L. A., M. Gonzalez, and O. T. Ramirez (2000) Evidence of Pluronic F-68 direct interaction with insect cells: impact on shear protection, recombinant protein, and baculovirus production. Enzyme Microb. Technol. 26: 324-331.
22. Kumar, V., L. Laouar, M. R. Davey, B. J. Mulligan, and K. C. Lowe (1990) Effects of Pluronic F-68 on growth of transformed roots of Solanum dulcamara. Biotechnol. Lett. 12: 937-940.
23. Bassetti, L., M. Hagendoorn, and J. Tramper (1995) Surfactant-induced non-lethal release of anthraquinones from suspension cultures of Morinda citrifolia. J. Biotechnol. 39: 149-155.
24. Laouar, L., K. C. Lowe, and 13. J. Mulligan (1996) Yeast responses to nonionic surfactants. Enzyme Microb. Technol. 18: 433-438.
25. Hellung-Larsen, P., F. Assaad, S. Pankratova, B. L. Saietz, and L. T. Skovgaard (2000) Effects of Pluronic F-68 on Tetrahymena cells: protection against chemical and physical stress and prolongation of survival under toxic conditions. J. Biotechnol. 76: 185-195.
26. Chattopadhyay, D., J. F. Rathman, and J. J. Chalmers (1995) The protective effect of specific medium additives with respect to bubble rupture. Biotechnol. Bioeng. 45: 473-480.