Identification and genetic fingerprinting of Brachyspira species

Journal of Microbiological Methods

Volumn 72, Issue 2, 2008, Pages 133-140

  Fellström, Claes ^a   Råsbäck, Therese ^a,b   Johansson, Karl Erik ^a,b   Olofsson, Tobias ^c   Aspán, Anna ^b  

^a SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

^b NATIONAL VETERINARY INSTITUTE   (Sweden)

^c LUND UNIVERSITY   (Sweden)

Author keywords

Brachyspira; Fingerprinting; Identification; Typing

Indexed keywords

RNA 16S;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; BRACHYSPIRA; BRACHYSPIRA HYODYSENTERIAE; BRACHYSPIRA INNOCENS; BRACHYSPIRA INTERMEDIA; BRACHYSPIRA PILOSICOLI; DNA FINGERPRINTING; GENE IDENTIFICATION; GENETIC VARIABILITY; MOLECULAR GENETICS; MOLECULAR PHYLOGENY; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPIC VARIATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; RANDOM AMPLIFIED POLYMORPHIC DNA; STRAIN DIFFERENCE;

ANIMALS; BACTERIAL TYPING TECHNIQUES; BRACHYSPIRA; DNA FINGERPRINTING; DNA, BACTERIAL; DNA, RIBOSOMAL; ELECTROPHORESIS, GEL, PULSED-FIELD; MOLECULAR SEQUENCE DATA; PHYLOGENY; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 23S; SEQUENCE ANALYSIS, DNA; SWINE;

BRACHYSPIRA; BRACHYSPIRA HYODYSENTERIAE; BRACHYSPIRA INNOCENS; BRACHYSPIRA INTERMEDIA; BRACHYSPIRA PILOSICOLI; SUS;

EID: 38049105807     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2007.11.015     Document Type: Article

References (48)

1. Atyeo R.F., Oxberry S.L., and Hampson D.J. Pulsed-field gel electrophoresis for sub-specific differentiation of Serpulina pilosicoli (formerly 'Anguillina coli'). FEMS Microbiol. Lett. 141 (1996) 77-81
2. Atyeo R.F., Oxberry S.L., and Hampson D.J. Analysis of Serpulina hyodysenteriae strain variation and its molecular epidemiology using pulsed-field gel electrophoresis. Epidemiol. Infect. 123 (1999) 133-138
3. Atyeo R.F., Stanton T.B., Jensen N.S., Suriyaarachichi D.S., and Hampson D.J. Differentiation of Serpulina species by NADH oxidase gene (nox) sequence comparisons and nox-based polymerase chain reaction tests. Vet. Microbiol. 67 (1999) 47-60
4. Binek M., and Szynkiewicz Z.M. Physiological properties and classification of strains of Treponema sp. isolated from pigs in Poland. Comp. Immunol. Microb. 7 (1984) 141-148
5. Calderaro A., Bommezzadri S., Gorrini C., Piccolo G., Peruzzi S., Dettori G., and Chezzi G. Comparative evaluation of molecular assays for the identification of intestinal spirochaetes from diseased pigs. Vet. Microbiol. 118 (2006) 91-100
6. Dugourd D., Jacques M., Bigras-Poulin M., and Harel J. Characterization of Serpulina hyodysenteriae isolates of serotypes 8 and 9 by random amplification of polymorphic DNA analysis. Vet. Microbiol. 48 (1996) 305-314
7. Duhamel G.E., Trott D.J., Muniappa N., Mathiesen M.R., Tarasiuk K., Lee J.I., and Hampson D.J. Canine intestinal spirochetes consist of Serpulina pilosicoli and a newly identified group provisionally designated "Serpulina canis" sp. nov. J. Clin. Microbiol. 36 (1998) 2264-2270
8. Dwars R.M., Smit H.F., and Davelaar F.G. Observations on avian intestinal spirochaetosis. Vet. Q. 12 (1990) 51-55
9. Fellström C., and Gunnarsson A. Phenotypical characterisation of intestinal spirochaetes isolated from pigs. Res. Vet. Sci. 59 (1995) 1-4
10. Fellström C., Pettersson B., Thomson J., Gunnarsson A., Persson M., and Johansson K.-E. Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR. J. Clin. Microbiol. 35 (1997) 462-467
11. Fellström C., Karlsson M., Pettersson B., Zimmerman U., Gunnarsson A., and Aspan A. Emended descriptions of indole negative and indole positive isolates of Brachyspira (Serpulina) hyodysenteriae. Vet. Microbiol. 70 (1999) 225-238
12. Fellström C., Zimmerman U., Aspan A., and Gunnarsson A. The use of culture, pooled samples and PCR for identification of herds infected with Brachyspira hyodysenteriae. Anim. Health Res. Rev. 2 (2001) 37-43
13. Fellström C., and Holmgren N. Mice as reservoirs for swine dysentery in a fattening herd. Sv. Vet. Tidn. 13 (2005) 19-21
14. Fossii M., Pohjanvirta T., and Pelkonen S. Molecular epidemiological study of Brachyspira pilosicoli in Finnish sow herds. Epidemiol. Infect. 131 (2003) 967-973
15. Fossi M., Pohjanvirta T., Sukura A., Heinikainen S., Lindecrona R., and Pelkonen S. Molecular and ultrastructural characterization of porcine hippurate-negative Brachyspira pilosicoli. J. Clin. Microbiol. 42 (2004) 3153-3158
16. Harris D.L., and Glock R.D. Swine dysentery. J. Am. Vet. Med. Assoc. 160 (1972) 561-565
17. Hommez J., Castryck F., Haesebrouck F., and Devriese L.A. Identification of porcine Serpulina strains in routine diagnostic bacteriology. Vet. Microbiol. 62 (1998) 163-169
18. Hudson M.J., Alexander T.J., and Lysons R.J. Diagnosis of swine dysentery: spirochaetes which may be confused with Treponema hyodysenteriae. Vet. Rec. 99 (1976) 498-500
19. Jansson D.S., Johansson K.-E., Olofsson T., Råsbäck T., Vågsholm I., Pettersson B., Gunnarsson A., and Fellström C. Brachyspira hyodysenteriae and other strongly beta-haemolytic and indole-positive spirochaetes isolated from mallards (Anas platyrhynchos). J. Med. Microbiol. 53 (2004) 293-300
20. Johansson K.E., Duhamel G.E., Bergsjø B., Engvall E.O., Persson M., Pettersson B., and Fellström C. Identification of three clusters of canine intestinal spirochaetes by biochemical and 16S rDNA sequence analysis. J. Med. Microbiol. 53 (2004) 345-350
21. Karlsson M., Aspán A., Landén A., and Franklin A. Further characterization of porcine Brachyspira hyodysenteriae isolates with decreased susceptibility to tiamulin. J. Med. Microbiol. 53 (2004) 281-285
22. Kinyon J.M., Harris D.L., and Glock R.D. Enteropathogenicity of various isolates of Treponema hyodysenteriae. Infect. Immun. 15 (1977) 638-646
23. Kinyon J.M., and Harris D.L. Treponema innocens, a new species of intestinal bacteria, and emended description of the type strain of Treponema hyodysenteriae. Int. J. Syst. Bacteriol. 29 (1979) 102-109
24. La T., Phillips N.D., and Hampson D.J. Development of a duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces. J. Clin. Microbiol. 41 (2003) 3372-3375
25. Lee J.I., Hampson D.J., Lymbery A.J., and Harders S.J. The porcine intestinal spirochaetes: identification of new genetic groups. Vet. Microbiol. 34 (1993) 273-285
26. Leser T.D., Moller K., Jensen T.K., and Jorsal S.E. Specific detection of Serpulina hyodysenteriae and potentially pathogenic weakly beta-haemolytic porcine intestinal spirochetes by polymerase chain reaction targeting 23S rDNA. Mol. Cell. Probes 11 (1997) 363-372
27. Ludwig W., and Klenk H.-P. Overview: a phylogenetic backbone and taxonomic framework for procaryotic systematics. In: Garrity G. (Ed). Bergey's Manual of Systematic Bacteriology (2001), Springer-Verlag, New York, NY 49-65
28. McLaren A.J., Hampson D.J., and Wylie S.L. The prevalence of intestinal spirochaetes in poultry flocks in Western Australia. Aust. Vet. J. 74 (1996) 319-321
29. McLaren A.J., Trott D.J., Swayne D.E., Oxberry S.L., and Hampson D.J. Genetic and phenotypic characterization of intestinal spirochetes colonizing chickens and allocation of known pathogenic isolates to three distinct genetic groups. J. Clin. Microbiol. 35 (1997) 412-417
30. Moller K., Jensen T.K., Jorsal S.E., Leser T.D., and Carstensen B. Detection of Lawsonia intracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonella enterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs. Vet. Microbiol. 62 (1998) 59-72
31. Neef N.A., Lysons R.J., Trott D.J., Hampson D.J., Jones P.W., and Morgan J.H. Pathogenicity of porcine intestinal spirochetes in gnotobiotic pigs. Infect. Immun. 62 (1994) 2395-2403
32. Olsen G.J., and Woese C.R. Ribosomal RNA: a key to phylogeny. FASEB J. 7 (1993) 113-123
33. Pettersson B., Fellström C., Andersson A., Uhlen M., Gunnarsson A., and Johansson K.E. The phylogeny of intestinal porcine spirochetes (Serpulina species) based on sequence analysis of the 16S rRNA gene. J. Bacteriol. 178 (1996) 4189-4199
34. Phillips N.D., La T., and Hampson D.J. Development of a two-step nested PCR assay for the rapid detection of Brachyspira pilosicoli and Brachyspira intermedia in chicken faeces. Vet. Microbiol. 116 (2006) 239-245
35. Quednau M., Ahrne S., Petersson A.C., and Molin G. Identification of clinically important species of Enterococcus within 1 day with randomly amplified polymorphic DNA (RAPD). Curr. Microbiol. 36 (1998) 332-336
36. Rohde J., Rothkamp A., and Gerlach G.F. Differentiation of porcine Brachyspira species by a novel nox PCR-based restriction fragment length polymorphism analysis. J. Clin. Microbiol. 40 (2002) 2598-2600
37. Råsbäck T., Fellström C., Bergsjø B., Cizek A., Collin K., Gunnarsson A., Jensen S.M., Mars A., Thomson J., Vyt P., and Pringle M. Assessment of diagnostics and antimicrobial susceptibility testing of Brachyspira species using a ring test. Vet. Microbiol. 109 (2005) 229-243
38. Råsbäck T., Jansson S.D., Johansson K.-E., and Fellström C. A novel enteropathogenic, strongly haemolytic spirochaete isolated from pig and mallard, provisionally designated 'Brachyspira suanatina' sp. nov. Environ. Microbiol. 9 (2007) 983-991
39. Råsbäck T., Johansson K.-E., Jansson D.S., Fellström C., Alikhani M.Y., La T., Dunn D.S., and Hampson D.J. Development of a multilocus sequence typing scheme for intestinal spirochaetes within the genus Brachyspira. Microbiol. 153 (2007) 4074-4087
40. Stanton T.B., Trott D.J., Lee J.I., McLaren A.J., Hampson D.J., Paster B.J., and Jensen N.S. Differentiation of intestinal spirochaetes by multilocus enzyme electrophoresis analysis and 16S rRNA sequence comparisons. FEMS Microbiol. Lett. 136 (1996) 181-186
41. Stanton T.B., Fournie-Amazouz E., Postic D., Trott D.J., Grimont P.A., Baranton G., Hampson D.J., and Saint Girons I. Recognition of two new species of intestinal spirochetes: Serpulina intermedia sp. nov. and Serpulina murdochii sp. nov. Int. J. Syst. Bacteriol. 47 (1997) 1007-1012
42. Stanton T.B., Rosey E.L., Kennedy M.J., Jensen N.S., and Bosworth B.T. Isolation, oxygen sensitivity, and virulence of NADH oxidase mutants of the anaerobic spirochete Brachyspira (Serpulina) hyodysenteriae, etiologic agent of swine dysentery. Appl. Environ. Microbiol. 65 (1999) 5028-5034
43. Suriyaarachchi D.S., Mikosza A.S., Atyeo R.F., and Hampson D.J. Evaluation of a 23S rDNA polymerase chain reaction assay for identification of Serpulina intermedia, and strain typing using pulsed-field gel electrophoresis. Vet. Microbiol. 71 (2000) 139-148
44. Townsend K.M., Giang V.N., Stephens C., Scott P.T., and Trott D.J. Application of nox-restriction fragment length polymorphism for the differentiation of Brachyspira intestinal spirochetes isolated from pigs and poultry in Australia. J. Vet. Diagn. Invest. 17 (2005) 103-109
45. Trott D.J., Mikosza A.S., Combs B.G., Oxberry S.L., and Hampson D.J. Population genetic analysis of Serpulina pilosicoli and its molecular epidemiology in villages in the eastern Highlands of Papua New Guinea. Int. J. Syst. Bacteriol. 48 (1998) 659-668
46. Welsh J., and McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic. Acids Res. 18 (1990) 7213-7218
47. Williams J.G., Kubelik A.R., Livak K.J., Rafalski J.A., and Tingey S.V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic. Acids Res. 18 (1990) 6531-6535
48. Zuerner R.L., and Stanton T.B. Physical and genetic map of the Serpulina hyodysenteriae B78T chromosome. J. Bacteriol. 176 (1994) 1087-1092