Application of nox-restriction fragment length polymorphism for the differentiation of Brachyspira intestinal spirochetes isolated from pigs and poultry in Australia

Journal of Veterinary Diagnostic Investigation

Volumn 17, Issue 2, 2005, Pages 103-109

  Townsend, Kirsty M ^a   Giang, Vo Ngan ^a   Stephens, Carol ^b   Scott, Paul T ^a   Trott, Darren J ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b DEPARTMENT OF AGRICULTURE AND FISHERIES   (Australia)

Author keywords

16S ribosomal DNA sequencing; Brachyspira; Nicotinamide adenine dinucleotide reduced oxidase; PCR

Indexed keywords

AVES; BACTERIA (MICROORGANISMS); BRACHYSPIRA; BRACHYSPIRA HYODYSENTERIAE; BRACHYSPIRA PILOSICOLI; GALLIFORMES; SPIROCHAETALES; SUIDAE; SUS SCROFA;

EID: 15944363859     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063870501700202     Document Type: Article

References (15)

1. Atyeo RF, Oxberry SL, Combs BG, Hampson DJ: 1998, Development and evaluation of polymerase chain reaction tests as an aid to diagnosis of swine dysentery and intestinal spirochaetosis. Lett Appl Microbiol 26:126-130.
2. Fellström C, Gunnarsson A: 1995, Phenotypic characterisation of intestinal spirochaetes isolated from pigs. Res Vet Sci 59:1-4.
3. Fellström C, Pettersson B, Uhlén M, et al.: 1995, Phylogeny of Serpulina based on sequence analyses of the 16S rRNA gene and comparison with a scheme involving biochemical classification. Res Vet Sci 59:5-9.
4. Fellström C, Pettersson B, Zimmerman U, et al.: 2001, Classification of Brachyspira spp. isolated from Swedish dogs. Anim Health Res Rev 2:75-82.
5. Lee JI, Hampson DJ: 1994, Genetic characterisation of intestinal spirochaetes and their association with disease. J Med Microbiol 40:365-371.
6. Lee JI, Hampson DJ, Lymbery AJ, Harders SJ: 1993, The porcine intestinal spirochaetes: identification of new genetic groups. Vet Microbiol 34:273-285.
7. Leser TD, Møller K, Jensen TK, Jorsal SE: 1997, Specific detection of Serpulina hyodysenteriae and potentially pathogenic weakly β-hemolytic spirochetes by polymerase chain reaction targeting. Mol Cell Probes 11:363-372.
8. McLaren AJ, Trott DJ, Swayne DE, et al.: 1997, Genetic and phenotypic characterization of intestinal spirochetes colonizing chickens and allocation of known pathogenic isolates to three distinct genetic groups. J Clin Microbiol 35:412-417.
9. Mikosza AS, La T, Margawani KR, et al.: 2001, PCR detection of Brachyspira aalborgi and Brachyspira pilosicoli in human faeces. FEMS Microbiol Lett 197:167-170.
10. Park NY, Chung CY, McLaren AJ, et al.: 1995, Polymerase chain reaction for identification of human and porcine spirochaetes recovered from cases of intestinal spirochaetosis. FEMS Microbiol Lett 125:225-230.
11. Pettersson B, Fellström C, Andersson A, et al.: 1996, The phylogeny of intestinal porcine spirochetes (Serpulina species) based on sequence analysis of the 16S rRNA gene. J Bacteriol 178:4189-4199.
12. Rohde J, Rothkamp A, Gerlach GF: 2002, Differentiation of porcine Brachyspira species by a novel nox PCR-based restriction fragment length polymorphism analysis. J Clin Microbiol 40:2598-2600.
13. Stanton TB, Trott DJ, Lee JI, et al.: 1996, Differentiation of intestinal spirochaetes by multilocus enzyme electrophoresis and 16S r RNA sequence comparisons. FEMS Microbiol Lett 136:181-186.
14. Stephens CP, Hampson DJ: 2001, Intestinal spirochete infections of chickens: a review of disease association, epidemiology and control. Anim Health Res Rev 2:83-91.
15. Suriyaarachchi DS, Mikosza AS, Atyeo RF, Hampson, DJ: 2000, Evaluation of a 23S rDNA polymerase chain reaction assay for identification of Serpulina intermedia, and strain typing using pulsed-field gel electrophoresis. Vet Microbiol 71:139-148.