Specific detection of Serpulina hyodysenteriae and potentially pathogenic weakly β-haemolytic porcine intestinal spirochetes by polymerase chain reaction targeting 23S rDNA

Molecular and Cellular Probes

Volumn 11, Issue 5, 1997, Pages 363-372

  Leser, Thomas D ^a   Møller, Kristian ^a   Jensen, Tim K ^a   Jorsal, Sven E ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

23S rDNA; Detection; Intestinal spirochetes; PCR

Indexed keywords

RIBOSOME DNA; RNA 23S;

ARTICLE; BIOCHEMISTRY; BRACHYSPIRA; HEMOLYSIS; INTESTINE FLORA; MAXIMUM LIKELIHOOD METHOD; NONHUMAN; PHYLOGENY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPIROCHETE; SWINE;

BACTERIA (MICROORGANISMS); BRACHYSPIRA; BRACHYSPIRA HYODYSENTERIAE; SPIROCHAETALES; SUIDAE; SUS SCROFA;

EID: 0031259982     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1006/mcpr.1997.0129     Document Type: Article

References (34)

1. Taylor D. J, Alexander T. J. L. The production of dysentery in swine by feeding cultures containing a spirochaete. British Veterinary Journal. 127:1971;58-61.
2. Harris D. L, Glock R. D, Christensen C. R, Kinyon J. M. Swine dysentery-1. Inoculation of pigs withTreponema hyodysenteriae. Veterinary Medicine of Small Animal Clinician. 67:1972;61-68.
3. Stanton T. B. Proposal to change the genus designationSerpulaSerpulinaSerpulina hyodysenteriaeSerpulina innocens. International Journal of Systematic Bacteriology. 42:1992;189-190.
4. Kinyon J. M, Harris D. L. Treponema innocensTreponema hyodysenteriaeet al. International Journal of Systematic Bacteriology. 29:1979;102-109.
5. Lee J. I, Hampson D. J. Genetic characterisation of intestinal spirochaetes and their association with disease. Journal of Medical Microbiology. 40:1994;365-371.
6. Fellström C, Gunnarsson A. Phenotypical characterisation of intestinal spirochaetes isolated from pigs. Research in Veterinary Science. 59:1995;1-4.
7. Lee J. I, Hampson D. J, Lymbery A. J, Harders S. J. The porcine intestinal spirochaetes: identification of new genetic groups. Veterinary Microbiology. 34:1993;273-285.
8. Pettersson B, Fellström C, Andersson A, Uhlén M, Gunnarsson A, Johansson K.-E. The phylogeny of intestinal porcine spirochetes (Serpulina. Journal of Bacteriology. 178:1996;4189-4199.
9. Trott D. J, Stanton T. B, Jensen N. S, Duhamel G. E, Johnson J. L, Hampson D. J. Serpulina pilosicoli. International Journal of Systematic Bacteriology. 46:1996;206-215.
10. Stanton T. B, Jensen N. S, Casey T. A, Tordoff L. A, Dewhirst F. E, Paster B. J. Reclassification ofTreponema hyodysenteriaeTreponema innocensSerpulaSerpula hyodysenteriaeSerpula innocens. International Journal of Systematic Bacteriology. 41:1991;50-58.
11. Taylor D. J, Simmons J. R, Laird H. M. Production of diarrhoea and dysentery in pigs by feeding pure cultures of a spirochaete differing fromTreponema hyodysenteriae. Veterinary Record. 106:1980;326-332.
12. Thomson J. R, Smith W. J, Murray B. M, McOrist S. Porcine colitis: results of challenge studies using strains of weakly beta-haemolytic spirochetes. Proceedings of the 14th IPVS Congress, Bologna, Italy, 7-10 July, 1996. Pp. 280. 1996.
13. Duhamel G. E, Muniappa N, Mathiesen M. R. Certain weakly β-haemolytic intestinal spirochetes are phenotypically and genotypically related to spirochetes associated with human and porcine intestinal spirochaetosis. Journal of Clinical Microbiology. 33:1995;2212-2215.
14. Lee J. I, McLaren A. J, Lymbery A. J, Hampson D. J. Human intestinal spirochetes are distinct fromSerpulina hyodysenteriae. Journal of Clinical Microbiology. 31:1993;16-21.
15. Binek M, Szynkiewicz Z. M. Physiological properties and classification of strains ofTreponema. Comparative Immunology, Microbiology and Infectious Diseases. 7:1984;141-148.
16. T. Journal of Bacteriology. 176:1994;1087-1092.
17. Elder R. O, Duhamel G. E, Schafer R. W, Mathiesen M. R, Ramanathan M. Rapid detection ofSerpulina hyodysenteriae. Journal of Clinical Microbiology. 32:1994;1497-1502.
18. Harel J, Forget C. DNA probe and polymerase chain reaction procedure for the specific detection ofSerpulina hyodysenteriae. Molecular and Cellular Probes. 9:1995;111-119.
19. Park N. Y, Chung C. Y, McLaren A. J, Atyeo R. F, Hampson D. J. Polymerase chain reaction for identification of human and porcine spirochaetes recovered from cases of intestinal spirochaetosis. FEMS Microbiology Letters. 125:1995;225-230.
20. Fellström C, Pettersson B, Thomson J, Gunnarsson A, Persson M, Johansson K.-E. Identification ofSerpulina. Journal of Clinical Microbiology. 35:1997;462-467.
21. Ludwig W, Dorn S, Springer N, Kirchhof G, Schleifer K.-H. PCR-based preparation of 23S rRNA-targeted group-specific polynucleotide probes. Applied and Environmental Microbiology. 60:1994;3236-3244.
22. Kilian M. A taxonomic study of the genusHaemophilus. Journal of General Microbiology. 93:1976;9-62.
23. Ausubel F. M, Brent R, Kingston R. E. Preparation and analysis of DNA. Current Protocols in Molecular Biology. (Ausubel, F. M., ed.) Pp. 2.4.1-2.4.5. New York: John Wiley & Sons. 1988.
24. Maidak B. L, Olsen G. J, Larsen N, Overbeek R, McCaughey M. J, Woese C. R. The ribosomal database project. Nucleic Acids Research. 24:1996;82-85.
25. Felsenstein J. PHYLIP: Phylogeny interference package (version 3.5c). Department of Genetics, University of Washington, Seattle. 1993.
26. Höpfl P, Ludwig W, Schleifer K.-H, Larsen N. The 23S ribosomal RNA higher-order structure ofPseudomonas cepacia. European Journal of Biochemistry. 185:1989;355-364.
27. Fox G. E, Wisotzkey J. D, Jurtshuk P. Jr. How close is close: 16S rRNA sequence identity may not be sufficient to guarantee species identity. International Journal of Systematic Bacteriology. 42:1992;166-170.
28. Amann R. I, Lin C, Key R, Montgomery L, Stahl D. A. Diversity amongFibrobacter. Systematic and Applied Microbiology. 15:1992;23-31.
29. Olsen G. J, Woese C. R. Ribosomal RNA: a key to phylogeny. The FASEB Journal. 7:1993;113-123.
30. Stackebrandt E, Goebel B. M. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. International Journal of Systematic Bacteriology. 44:1994;846-849.
31. Kwok S, Kellogg D. E, McKinney N. Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies. Nucleic Acids Research. 18:1990;999-1005.
32. Deuter R, Pietsch S, Hertel S, Müller O. A method for preparation of faecal DNA suitable for PCR. Nucleic Acids Research. 23:1995;3800-3801.
33. Kunkle R. A, Kinyon J. M. Improved selective medium for the isolation ofTreponema hyodysenteriae. Journal of Clinical Microbiology. 26:1988;2357-2360.
34. Stacy-Phipps S, Mecca J. J, Weiss J. B. Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenicEscherichia coli. Journal of Clinical Microbiology. 33:1995;1054-1059.