Use of a newly developed β-mercaptoethanol enzyme-linked immunosorbent assay to diagnose visceral leishmaniasis in patients in Eastern Sudan

Clinical and Vaccine Immunology

Volumn 14, Issue 12, 2007, Pages 1592-1595

  Mansour, Durria ^a   Abass, Elfadil M ^a   El Mutasim, Mohamed ^a,c   Mahamoud, Abdelhafeiz ^a   El Harith, Abdallah ^a,b  

^a AHFAD UNIVERSITY FOR WOMEN   (Sudan)

^b Aljouf College of Health Sciences for Boys ^*  (Netherlands)

^c Laboratory Department   (Saudi Arabia)

Author keywords

[No Author keywords available]

Indexed keywords

ANTILEISHMANIAL AGENT; MERCAPTOETHANOL; STIBOGLUCONATE SODIUM;

AGGLUTINATION TEST; ANTIGEN SPECIFICITY; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; DRUG RESPONSE; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; HUMAN CELL; LEISHMANIA DONOVANI; LYMPH NODE BIOPSY; MAJOR CLINICAL STUDY; PRIORITY JOURNAL; SEROLOGY; SUDAN; SYMPTOMATOLOGY; VISCERAL LEISHMANIASIS;

AGGLUTINATION TESTS; ANIMALS; ANTIBODIES, PROTOZOAN; ANTIGENS, PROTOZOAN; CASE-CONTROL STUDIES; ENDEMIC DISEASES; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOGLOBULIN G; LEISHMANIA DONOVANI; LEISHMANIASIS, VISCERAL; MERCAPTOETHANOL; REAGENT STRIPS; REDUCING AGENTS; RURAL POPULATION; SENSITIVITY AND SPECIFICITY; STATISTICS AS TOPIC; SUDAN;

EID: 38049073194     PISSN: 15566811     EISSN: 1556679X     Source Type: Journal    
DOI: 10.1128/CVI.00313-07     Document Type: Article

References (17)

1. Abass, E. M., D. Mansour, M. el Mutasim, M. Hussein, and A. el Harith 2006. β-Mercaptoethanol-modified ELISA for diagnosis of visceral leishmaniasis. J. Med. Microbiol. 55:1193-1196.
2. Boelaert, M., S. H. El Safi, D. Jacquet, A. de Muynck, P. van der Stuyft, and D. Le Ray. 1999. Operational validation of the direct agglutination test for diagnosis of visceral leishmaniasis. Am. J. Trop. Med. Hyg. 60:129-134.
3. Chappius, F., A. Rijal, J. Menten, and M. Boelaert. 2006. A meta-analysis of the diagnostic performance of the direct agglutination test and rK39 dipstick for visceral leishmaniasis. Br. Med. J. 333:723-726.
4. el Harith, A., A. H. J. Kolk, P. A. Kager, J. Leeuwenburg, F. J. Faber, R. Muigai, S. Kiugu, and J. J. Laarman. 1987. Evaluation of a newly developed direct agglutination test (DAT) for serodiagnosis and sero-epidemiological studies of visceral leishmaniasis: comparison with IFAT and ELISA. Trans. R. Soc. Trop. Med. Hyg. 81:603-606.
5. el Harith, A., A. H. J. Kolk, J. Leeuwenburg, R. Muigai, E. Huigen, T. Jelsma, and P. A. Kager. 1988. Improvement of a direct agglutination test for field studies of visceral leishmaniasis. J. Clin. Microbiol. 26:1321-1325.
6. el Harith, A., S. Chowdhury, M. Al-Masum, S. J. Semiao-Santos, E. Karim, S. H. El-Safi, and I. Haque. 1995. Evaluation of cleaving agents other than trypsin in direct agglutination test for further improving diagnosis of visceral leishmaniasis. J. Clin. Microbiol. 33:1984-1988.
7. el Harith, A., M. El Mutasim, D. Mansour, E. F. Mustafa, I. Shammat, I. Gilis, and T. van Gool. 2002. National and international evaluation of in-Sudan produced direct agglutination test for visceral leishmaniasis. Sudan Med. J. 40:3-11.
8. el Harith, A., M. el Mutasim, D. Mansour, E. F. Mustafa, and A. Arvidson. 2003. Use of glycerol as alternative to freeze-drying for long-term preservation of antigen for the direct agglutination test. Trop. Med. Int. Health 8:1025-1029.
9. el Mutasim, M., D. Mansour, E. M. Abass, W. Hassan, and A. el Harith. 2006. Evaluation of a glycerol-preserved antigen in the direct agglutination test for diagnosis of visceral leishmaniasis in eastern Sudan. J. Med. Microbiol. 55:1343-1347.
10. El-Safi, S. H., A. Abdel-Haleem, A. Hammad, I. El-Basha, A. Omer, H. G. Kareem, M. Boelaert, M. Chance, and M. Hommel. 2003. Field evaluation of latex agglutination test for detecting urinary antigens in visceral leishmaniasis in Sudan. East Mediterranean Health J. 9:844-855.
11. Osman, O. F., P. A. Kager, and L. Oskam. 2000. Leishmaniasis in the Sudan: a literature review with emphasis on clinical aspects. Trop. Med. Int. Health 5:553-562.
12. Ritmeijer, K., Y. Melaku, M. Mueller, S. Kipngetich, C. O'Keeffe, and R. N. Davidson. 2006. Evaluation of a new recombinant K39 rapid diagnostic test for Sudanese visceral leishmaniasis. Am. J. Trop. Med. Hyg. 74:76-80.
13. Seaman, J., R. W. Ashford, J. Schorscher, and J. Dereure. 1992. Visceral leishmaniasis in Southern Sudan: status of healthy villagers in epidemic conditions. Ann. Trop. Med. Parasitol. 86:481-486.
14. Veeken, H., K. Ritmeijer, J. Seaman, and R. Davidson. 2003. Comparison of an rK39 dipstick rapid test with direct agglutination test and splenic aspiration for the diagnosis of kala-azar in Sudan. Trop. Med. Int. Health 8:164-167.
15. World Health Organization. 2004. Report of the Scientific Working Group meeting on leishmaniasis. TDR/SWG/04. http://www.who.int/tdr/publications/ publications/pdf/swg_leish.pdf
16. Zijlstra, E. E., N. S. Daifalla, P. A. Kager, E. A. G. Khalil, A. M. El-Hassan, S. G. Reed, and H. W. Ghalib. 1998. rK39 enzyme-linked immunosorbent assay for diagnosis of Leishmania donovani infection. Clin. Diagn. Lab. Immunol. 5:717-720.
17. Zijlstra, E. E., Y. Nur, P. Desjeux, E. A. G. Khalil, A. M. El-Hassan, and J. Groen. 2001. Diagnosing visceral leishmaniasis with the recombinant K39 strip test: experience from the Sudan. Trop. Med. Int. Health 6:108-113.