Ordered Multi-site Phosphorylation of the Splicing Factor ASF/SF2 By SRPK1

Journal of Molecular Biology

Volumn 376, Issue 1, 2008, Pages 55-68

  Ma, Chen Ting ^a   Velazquez Dones, Adolfo ^b   Hagopian, Jonathan C ^a   Ghosh, Gourisankar ^b   Fu, Xiang Dong ^c   Adams, Joseph A ^a  

^a San Diego   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

kinase; kinetics; phosphorylation; splicing; SR protein

Indexed keywords

ARGININE; PROTEIN KINASE; SERINE;

ALTERNATIVE RNA SPLICING; ARTICLE; CONTROLLED STUDY; GENE DELETION; MUTATION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN MODIFICATION; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING;

EID: 38049045547     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2007.08.029     Document Type: Article

References (29)

1. Stojdl D.F., and Bell J.C. SR protein kinases: the splice of life. Biochem. Cell. Biol. 77 (1999) 293-298
2. Graveley B.R. Alternative splicing: increasing diversity in the proteomic world. Trends Genet. 17 (2001) 100-107
3. Johnson J.M., Castle J., Garrett-Engele P., Kan Z., Loerch P.M., Armour C.D., et al. Genome-wide survey of human alternative pre-mRNA splicing with exon junction microarrays. Science 302 (2003) 2141-2144
4. Jurica M.S., and Moore M.J. Pre-mRNA splicing: awash in a sea of proteins. Mol. Cell 12 (2003) 5-14
5. Valcarcel J., and Green M.R. The SR protein family: pleiotropic functions in pre-mRNA splicing. Trends Biochem. Sci. 21 (1996) 296-301
6. Zahler A.M., Lane W.S., Stolk J.A., and Roth M.B. SR proteins: a conserved family of pre-mRNA splicing factors. Genes Dev. 6 (1992) 837-847
7. Xiao S.H., and Manley J.L. Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2. EMBO J. 17 (1998) 6359-6367
8. Xiao S.H., and Manley J.L. Phosphorylation of the ASF/SF2 RS domain affects both protein-protein and protein-RNA interactions and is necessary for splicing. Genes Dev. 11 (1997) 334-344
9. Jamison S.F., Pasman Z., Wang J., Will C., Luhrmann R., Manley J.L., and Garcia-Blanco M.A. U1 snRNP-ASF/SF2 interaction and 5′ splice site recognition: characterization of required elements. Nucleic Acids Res. 23 (1995) 3260-3267
10. Lai M.C., and Tarn W.Y. Hypophosphorylated ASF/SF2 binds TAP and is present in messenger ribonucleoproteins. J. Biol. Chem. 279 (2004) 31745-31749
11. Huang Y., Gattoni R., Stevenin J., and Steitz J.A. SR splicing factors serve as adapter proteins for TAP-dependent mRNA export. Mol. Cell 11 (2003) 837-843
12. Huang Y., and Steitz J.A. Splicing factors SRp20 and 9G8 promote the nucleocytoplasmic export of mRNA. Mol. Cell 7 (2001) 899-905
13. Ngo J.C., Chakrabarti S., Ding J.H., Velazquez-Dones A., Nolen B., Aubol B.E., et al. Interplay between SRPK and Clk/Sty kinases in phosphorylation of the splicing factor ASF/SF2 is regulated by a docking motif in ASF/SF2. Mol. Cell 20 (2005) 77-89
14. Colwill K., Pawson T., Andrews B., Prasad J., Manley J.L., Bell J.C., and Duncan P.I. The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution. EMBO J. 15 (1996) 265-275
15. Hall L.L., Smith K.P., Byron M., and Lawrence J.B. Molecular anatomy of a speckle. Anat. Rec. Discov. Mol. Cell. Evol. Biol. 288 (2006) 664-675
16. Aubol B.E., Chakrabarti S., Ngo J., Shaffer J., Nolen B., Fu X.D., et al. Processive phosphorylation of alternative splicing factor/splicing factor 2. Proc. Natl. Acad. Sci. U. S. A. 100 (2003) 12601-12606
17. Velazquez-Dones A., Hagopian J.C., Ma C.T., Zhong X.Y., Zhou H., Ghosh G., et al. Mass spectrometric and kinetic analysis of ASF/SF2 phosphorylation by SRPK1 and Clk/Sty. J. Biol. Chem. 280 (2005) 41761-41768
18. Adams J.A. Kinetic and catalytic mechanisms of protein kinases. Chem. Rev. 101 (2001) 2271-2290
19. Colwill K., Feng L.L., Yeakley J.M., Gish G.D., Caceres J.F., Pawson T., and Fu X.D. SRPK1 and Clk/Sty protein kinases show distinct substrate specificities for serine/arginine-rich splicing factors. J. Biol. Chem. 271 (1996) 24569-24575
20. Huang Y., Yario T.A., and Steitz J.A. A molecular link between SR protein dephosphorylation and mRNA export. Proc. Natl. Acad. Sci. U. S. A. 101 (2004) 9666-9670
21. Koizumi J., Okamoto Y., Onogi H., Mayeda A., Krainer A.R., and Hagiwara M. The subcellular localization of SF2/ASF is regulated by direct interaction with SR protein kinases (SRPKs). J. Biol. Chem. 274 (1999) 11125-11131
22. Massiello A., and Chalfant C.E. SRp30a (ASF/SF2) regulates the alternative splicing of caspase-9 pre-mRNA and is required for ceramide-responsiveness. J. Lipid Res. 47 (2006) 892-897
23. Ding J.H., Zhong X.Y., Hagopian J.C., Cruz M.M., Ghosh G., Feramisco J., et al. Regulated cellular partitioning of SR protein-specific kinases in mammalian cells. Mol. Biol. Cell 17 (2006) 876-885
24. Fiol C.J., Wang A., Roeske R.W., and Roach P.J. Ordered multisite protein phosphorylation. Analysis of glycogen synthase kinase 3 action using model peptide substrates. J. Biol. Chem. 265 (1990) 6061-6065
25. Fiol C.J., Haseman J.H., Wang Y.H., Roach P.J., Roeske R.W., Kowalczuk M., and DePaoli-Roach A.A. Phosphoserine as a recognition determinant for glycogen synthase kinase-3: phosphorylation of a synthetic peptide based on the G-component of protein phosphatase-1. Arch. Biochem. Biophys. 267 (1988) 797-802
26. Fiol C.J., Mahrenholz A.M., Wang Y., Roeske R.W., and Roach P.J. Formation of protein kinase recognition sites by covalent modification of the substrate. Molecular mechanism for the synergistic action of casein kinase II and glycogen synthase kinase 3. J. Biol. Chem. 262 (1987) 14042-14048
27. Hernandez F., Perez M., Lucas J.J., Mata A.M., Bhat R., and Avila J. Glycogen synthase kinase-3 plays a crucial role in tau exon 10 splicing and intranuclear distribution of SC35. Implications for Alzheimer's disease. J. Biol. Chem. 279 (2004) 3801-3806
28. Patwardhan P., Shen Y., Goldberg G.S., and Miller W.T. Individual Cas phosphorylation sites are dispensable for processive phosphorylation by Src and anchorage-independent cell growth. J. Biol. Chem. 281 (2006) 20689-20697
29. Yeakley J.M., Tronchere H., Olesen J., Dyck J.A., Wang H.Y., and Fu X.D. Phosphorylation regulates in vivo interaction and molecular targeting of serine/arginine-rich pre-mRNA splicing factors. J. Cell Biol. 145 (1999) 447-455