Selective gene amplification

Protein Engineering, Design and Selection

Volumn 20, Issue 12, 2007, Pages 577-581

  Kelly, Bernard T ^a,c   Griffiths, Andrew D ^a,b  

^a MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

^b UNIVERSITÉ LOUIS PASTEUR   (France)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Directed evolution; Emulsion; In vitro compartmentalisation; IVC; PCR

Indexed keywords

EMULSIONS; ENZYME ACTIVITY; LIGANDS;

DIRECTED EVOLUTION; IN VITRO COMPARTMENTALIZATION;

GENES;

ANTIBODY;

ARTICLE; CELL COMPARTMENTALIZATION; GENE AMPLIFICATION; IN VITRO STUDY; NATURAL SELECTION; PRIORITY JOURNAL; PROGENY; SELECTIVE GENE AMPLIFICATION;

ANTIBODIES; CARBON-NITROGEN LIGASES; CATALYSIS; DIRECTED MOLECULAR EVOLUTION; EMULSIONS; ESCHERICHIA COLI PROTEINS; GENE AMPLIFICATION; GENE LIBRARY; GENETIC TECHNIQUES; GLUTATHIONE TRANSFERASE; LIGANDS; PHOSPHORIC TRIESTER HYDROLASES; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING; PSEUDOMONAS; REPRESSOR PROTEINS; RNA, CATALYTIC; TRANSCRIPTION FACTORS;

EID: 37849000493     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzm060     Document Type: Article

References (17)

1. Agresti,J.J., Kelly,B.T., Jaschke,A. and Griffiths,A.D. (2005) Proc. Natl Acad. Sci. USA, 102, 16170-16175.
2. Aharoni,A., Amitai,G., Bernath,K., Magdassi,S. and Tawfik,D.S. (2005) Chem. Biol., 12, 1281-1289.
3. Anna,S.L., Bontoux,N. and Stone,H.A. (2003) Appl. Phys. Lett., 82, 364-366.
4. Cohen,H.M., Tawfik,D.S. and Griffiths,A.D. (2004) Protein Eng. Des. Sel., 17, 3-11.
5. Doi,N., Kumadaki,S., Oishi,Y., Matsumura,N. and Yanagawa,H. (2004) Nucleic Acids Res., 32, e95.
6. Ghadessy,F.J., Ong,J.L. and Holliger,P. (2001) Proc. Natl Acad. Sci. USA, 98, 4552-4557.
7. Ghadessy,F.J., Ramsay,N., Boudsocq,F., Loakes,D., Brown,A., Iwai,S., Vaisman,A., Woodgate,R. and Holliger,P. (2004) Nat. Biotechnol., 22, 755-759.
8. Griffiths,A.D. and Tawfik,D.S. (2003) EMBO J., 22, 24-35.
9. Griffiths,A.D. and Tawfik,D.S. (2000) Curr. Opin. Biotechnol., 11, 338-353.
10. Griffiths,A.D. and Tawfik,D.S. (2006) Trends Biotechnol., 24, 395-402.
11. Lee,Y.F., Tawfik,D.S. and Griffiths,A.D. (2002) Nucleic Acids Res., 30, 4937-4944.
12. Levy,M., Griswold,K.E. and Ellington,A.D. (2005) RNA, 11, 1555-1562.
13. Mastrobattista,E., Taly,V., Chanudet,E., Treacy,P., Kelly,B.T. and Griffiths,A.D. (2005) Chem. Biol., 12, 1291-1300.
14. Miller,O.J., Bernath,K., Agresti,J.J., Amitai,G., Kelly,B.T., Mastrobattista,E., Taly,V., Magdassi,S., Tawfik,D.S. and Griffiths,A.D. (2006) Nat. Methods, 3, 561-570.
15. Raushel,F.M. and Holden,H.M. (2000) Adv. Enzymol. Relat. Areas Mol. Biol., 74, 51-93.
16. Tawfik,D.S. and Griffiths,A.D. (1998) Nat. Biotechnol., 16, 652-656.
17. Zaher,H.S. and Unrau,P.J. (2007) RNA, 13, 1017-1026.