Ethanol-induced cytotoxicity in rat pancreatic acinar AR42J cells: Role of fatty acid ethyl esters

Alcohol and Alcoholism

Volumn 43, Issue 1, 2008, Pages 1-8

  Wu, Hai ^a   Bhopale, Kamlesh K ^a   Ansari, G A S ^a   Kaphalia, Bhupendra S ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL; AMYLASE; CASPASE 3; CASPASE 3 INHIBITOR; FATTY ACID ESTER; TRIACYLGLYCEROL LIPASE;

ACINAR CELL; ALCOHOLIC PANCREATITIS; ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CULTURE; CELL STRAIN; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; ENZYME ACTIVITY; ENZYME INHIBITION; FATTY ACID SYNTHESIS; INCUBATION TIME; LIPOGENESIS; NONHUMAN; PANCREAS INJURY; PRIORITY JOURNAL; RAT;

ANIMALS; APOPTOSIS; CELL LINE, TUMOR; CYTOTOXINS; DOSE-RESPONSE RELATIONSHIP, DRUG; ESTERS; ETHANOL; FATTY ACIDS; HUMANS; PANCREAS, EXOCRINE; RATS;

RATTUS;

EID: 37749028739     PISSN: 07350414     EISSN: 14643502     Source Type: Journal    
DOI: 10.1093/alcalc/agm044     Document Type: Article

References (52)

1. Aleryani, S., Kabakibi, A., Cluette-Brown, J. and Laposata, M. (1996) Fatty acid ethyl ester synthase, an enzyme for nonoxidative ethanol metabolism, is present in serum after liver and pancreatic injury. Clinical Chemistry 42, 24-27.
2. Bailey, J. M., Gallo, L. L. and Gillespie, J. (1995) Inhibition of dietary cholesterol ester absorption by 3-BCP, a suicide inhibitor of cholesterol esterase. Biochemical Society Transactions 23, 408S.
3. Berild, D. and Hasselbalch, H. (1981) Survival after a blood alcohol of 1127 mg/dL. Lancet 2, 363.
4. Bhat, H. K. and Ansari, G. A. S. (1990) Cholesterol ester hydrolase-mediated conjugation of haloethanols with fatty acids. Chemical Research in Toxicology 3, 311-317.
5. Bhopale, K., Wu, H., Boor, P. J. et al. (2006) Metabolic basis of ethanol-induced hepatic and pancreatic toxicity in hepatic alcohol dehydrogenase deficient deer mice. Alcohol 39, 179-188.
6. Christophe, J. (1994) Pancreatic tumoral cell line AR42J. An amophicrine model. American Journal of Physiology 266, G963-G971.
7. Christopoulos, G., Kirch, E. R. and Gearien, J. E. (1973) Determination of ethanol in fresh and putrified post mortem tissues. Journal of Chromatography 87, 454-472.
8. Chrostek, L., Jelski, W., Szmitkowski, M. and Puchalski, Z. (2003) Alcohol dehydrogenase (ADH) isoenzymes and aldehyde dehydrogenase (ALDH) activity in the human pancreas. Digestive Diseases and Sciences 48, 1230-1233.
9. Criddle, D. N., Raraty, M. G., Neoptolemos, J. P. et al. (2004) Ethanol toxicity in pancreatic acinar cells: Mediation by nonoxidative fatty acid metabolites. Proceedings of the National Academy of Sciences of the United States of America 101, 10738-10743.
10. Erlanson, C. (1970) p-Nitrophenylacetate as a substrate for carboxylester hydrolase in pancreatic juice and intestinal content. Scandinavian Journal of Gastroenterology 5, 333-336.
11. Foster, J. R., Idle, J. R., Hartwich, J. P. et al. (1993) Induction of drug-metabolizing enzymes in human pancreatic cancer and chronic pancreatitis. Journal of Pathology 169, 457-463.
12. Gallo, L. L., Cheriathundam, E., Vahouny, G. V. and Treadwell, C. R. (1978) Immunological comparison of cholesterol esterases. Archives of Biochemistry and Biophysics 191, 42-48.
13. Hammond, K. B., Rumack, B. H. and Rodgerson, D. O. (1973) Blood ethanol, A report of unusually high levels in a living patient. Journal of the American Medical Association 226, 63-64.
14. Huang, Y. and Hui, D. Y. (1994) Synergistic effects of bombesin and cholecystokinin on cholesterol esterase biosynthesis and secretion by AR42J cells. Archives of Biochemistry and Biophysics 310, 54-59.
15. Hurley, P. R., Cook, A., Jehanli, A. et al. (1988) Development of radioimmunoassay for free tetra-L-aspartyl-L-lysine trypsinogen activation peptides [TAP]. Journal of Immunological Methods 111 195-203.
16. Kaphalia, B. S. and Ansari, G. A. S. (2001a) Purification and characterization of rat hepatic microsomal low molecular weight fatty acid ethyl ester synthase and their relationship to carboxylesterase. Journal of Biochemistry and Molecular Toxicology 15, 165-171.
17. Kaphalia, B. S. and Ansari, G. A. S. (2001b) Fatty acid ethyl esters and ethanol-induced pancreatic injury. Cellular and Molecular Biology 47, OL173-OL179.
18. Kaphalia, B. S. and Ansari, G. A. S. (2003) Purification and characterization of rat pancreatic fatty acid ethyl ester synthase and its structural and functional relationship to pancreatic cholesterol esterase. Journal of Biochemistry and Molecular Toxicology 17 338-345.
19. Kaphalia, B. S., Fritz, R. R. and Ansari, G. A. S. (1997) Purification and characterization of rat liver microsomal fatty acid ethyl and 2-chloroethyl ester synthase and their relationship with carboxylesterase (pI 6.1). Chemical Research in Toxicology 10 211-218.
20. Kaphalia, B. S., Green, S. M. and Ansari, G. A. S. (1999) Fatty acid ethyl and methyl ester synthases, and fatty acid anilide synthase in HepG2 and AR42J cells: Interrelationships and inhibition by tri-o-tolyl phosphate. Toxicology and Applied Pharmacology 159, 134-141.
21. Kaphalia, B. S., Khan, M. F., Boor, P. J. and Ansari, G. A. S. (1992) Toxic response to repeated oral administration of 2-chloroethyl linoleate in rats. Research Communications in Chemical Pathology and Pharmacology 76, 209-222.
22. Kaphalia, B. S., Khan, M. F., Carroll, R. M. et al. (1996) Subchronic toxicity of 2-chloroethanol and 2-bromoethanol in rats. Research Communication in Pharmacology and Toxicology 1, 173-186.
23. Kaphalia, B. S., Mericle, K. A. and Ansari, G. A. S. (2004a) Mechanism of differential inhibition of hepatic and pancreatic fatty acid ethyl ester synthase by inhibitors of serine-esterases: In vitro and cell culture studies. Toxicology and Applied Pharmacology 200, 7-15.
24. Kaphalia, B. S., Cai, P., Khan, M. F. et al. (2004b) Fatty acid ethyl esters in alcoholics; markers of alcohol abuse and alcoholism. Alcohol 34, 151-158.
25. Kessova, I. G., DeCarli, L. M. and Lieber, C. S. (1998) Inducibility of cytochrome P4502E1 and P4501A1 in the rat pancreas. Alcoholism-Clinical and Experimental Research 22, 501-504.
26. Lankisch, P. G. and Banks, P. A. (1998.) Pancreatitis, pp. 377. Springer-Verlag, Berlin, Heidelberg, New York.
27. Lange, L. G. and Sobel, B. E. (1983) Mitochondrial dysfunction induced by fatty acid ethyl esters, myocardial metabolites of ethanol. Journal of Clinical Investigation 72, 724-731.
28. Laposata, E. A. and Lange, L. G. (1986) Presence of nonoxidative ethanol metabolism in humans organs commonly damaged by ethanol abuse. Science 231, 497-499.
29. Lindblad, B. and Olsson, R. (1951) Unusually high levels of blood alcohol. Journal of the American Medical Association 236, 1600-1602.
30. Manautou, J. E. and Carlson, G. P. (1991) Ethanol-induced fatty acid ethyl ester formation in vivo and in vitro in rat lung. Toxicology 70, 303-312.
31. Norton, I. D., Apte, M. V., Haber, P. S. et al. (1998) Cytochrome P4502E1 is present in rat pancreas and is induced by chronic ethanol administration. Gut 42, 426-430.
32. Nuutinen, H., Lindros, K. O. and Salaspuro, M. (1983) Determinants of blood acetaldehyde level during ethanol oxidation in chronic alcoholics. Alcoholism-Clinical and Experimental Research 7, 163-168.
33. Palmer, K. R. and Jenkins, W. J. (1985) Aldehyde dehydrogenase in alcoholic subjects. Hepatology 5, 260-263.
34. Pandol, S. J., Periskic, S., Gukovsky, I. et al. (1999) Ethanol diet increases the sensitivity of rats to pancreatitis by cholecystokinin octapeptide. Gastroenterology 117, 706-716.
35. Panes, J., Soler, X., Pares, A. et al. (1989) Influence of liver disease on hepatic alcohol and aldehyde dehydrogenases. Gastroenterology 97, 708-714.
36. Panes, J., Caballeria, J., Guitart, R. et al. (1993) Determinants of ethanol and acetaldehyde metabolism in chronic alcoholics. Alcoholism-Clinical and Experimental Research 17, 48-53.
37. Pfutzer, R. H., Tadic, S. D., Li, H. S. et al. (2002) Pancreatic cholesterol esterase, ES-10, and fatty acid ethyl ester synthase III gene expression are increased in the pancreas and liver but not in the brain or heart with long-term ethanol feeding in rats. Pancreas 25, 101-106.
38. Pitchumoni, C. S., Glasser, M., Saran, R. M. et al. (1984) Pancreatic fibrosis in chronic alcoholics and non alcoholics without clinical pancreatitis. American Journal of Gastroenterology 79 382-388.
39. Riley, D. J., Kyger, E. M., Spilburg, C. A. and Lange, L. G. (1990) Pancreatic cholesterol esterases. 2. Purification and characterization of human pancreatic fatty acid ethyl ester synthase. Biochemistry 29, 3848-3852.
40. Rognstead, R. and Grunnet, N. (1979) Enzymatic pathways of ethanol metabolism. In Biochemistry and Pharmacology of Ethanol, Majchrowicz., E., Noble, E. P. eds, pp. 65-86. Plenum Press, New York.
41. Schmidt, J., Fernandez-del Castillo, C., Rattner, D. W. et al. (1992) Trypsinogen activation peptide in experimental rat pancreatitis: prognostic implications and histopathologic correlates. Gastroenterology 103, 1009-1016.
42. Schneider, A., Whitcomb, D. C. and Singer, M. V. (2002) Animal models in alcoholic pancreatitis-What can we learn. Pancreatology 2, 189-203.
43. Sharkawi, M. (1984) In vivo inhibition of liver alcohol dehydrogenase by ethanol administration. Life Sciences 35, 2353-2357.
44. Shore, J. D. and Theorell, H. (1966) Substrate inhibition effects in the liver alcohol dehydrogenase reaction. Archives of Biochemistry and Biophysics 117, 375-380.
45. Singh, M. and Simsek, H. (1990) Ethanol and the pancreas. Gastroenterology 98, 1051-1062.
46. Strassnig, S. and Lankmayr, E. P. (1999) Elimination of matrix effects for static headspace analysis of ethanol. Journal of Chromatography 849, 629-636.
47. Werner, J., Laposata, M., Fernandez-del Castillo, C. et al. (1997) Pancreatic injury in rats induced by fatty acid ethyl ester, a nonoxidative metabolite of alcohol. Gastroenterology 113, 286-294.
48. Werner, J., Saghir, M., Warshaw, A. L. et al. (2002) Alcoholic pancreatitis in rats: Injury from nonoxidative metabolites of ethanol. American Journal of Physiology-Gastroenterology and Liver Physiology 283, G65-G73.
49. Wilson, J. S. and Apte, M. V. (2003) Role of alcohol metabolism in alcoholic pancreatitis. Pancreas 27, 311-315.
50. Wilson, J. S., Korsten, M. A., Apte, M. V. et al. (1990) Both ethanol consumption and protein deficiency increase the fragility of pancreatic lysosomes. Journal of Laboratory Clinical Medicine 115, 749-755.
51. Wu, H., Cai, P., Clemens, D. L. et al. (2006) Metabolic basis of ethanol-induced cytotoxicity in recombinant HepG2 Cells: Role of nonoxidative metabolism. Toxicology and Applied Pharmacology 216, 238-247.
52. Zahlten, R. N., Jacobson, C. J. and Neijtek, M. E. (1980) Under estimation of alcohol dehydrogenase as a result of various technical pitfalls of the enzyme assay. Biochemical Pharmacology 29, 1973-1976.