Fatty acid ethyl ester synthase, an enzyme for nonoxidative ethanol metabolism, is present in serum after liver and pancreatic injury

Clinical Chemistry

Volumn 42, Issue 1, 1996, Pages 24-27

  Aleryani, Samir ^a   Kabakibi, Ayman ^a   Cluette Brown, Joanne ^a   Laposata, Michael ^a,b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

chromatography, thin layer; enzyme activity; fatty acids; gas chromatography mass spectrometry; lipids

Indexed keywords

ALCOHOL; AMYLASE; ASPARTATE AMINOTRANSFERASE; BILIRUBIN; BILIRUBIN GLUCURONIDE; FATTY ACID ESTER; FATTY ACID ETHYL ESTER SYNTHASE; LIVER ENZYME; PANCREAS ENZYME; SYNTHETASE; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

ALCOHOL METABOLISM; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BLOOD LEVEL; GAS CHROMATOGRAPHY; HUMAN; HUMAN TISSUE; LIVER INJURY; MASS SPECTROMETRY; PANCREAS INJURY; THIN LAYER CHROMATOGRAPHY;

EID: 0030052919     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/42.1.24     Document Type: Article

References (18)

1. Lange LG, Bergmann SR, Sobel BE. Identification of fatty acid ethyl esters as products of rabbit myocardial ethanol metabolism. J Biol Chem 1981;256:129668-73.
2. Patton S, McCarthy RD. Conversion of alcohol to ethyl esters of fatty acids by lactating goat. Nature 1966;209:616-7.
3. Lange LG, Sobel BE. Mitochondrial dysfunction induced by fatty acid ethyl esters, myocardial metabolites of ethanol. J Clin Invest 1983;72:724-31.
4. Laposata EA, Lange LG. Presence of nonoxidative ethanol metabolism in human organs commonly damaged by ethanol abuse. Science 1986;231:497-9.
5. Mogelson S, Lange LG. Nonoxidative ethanol metabolism in rabbit myocardium: purification to homogeneity of fatty acid ethyl ester synthase. Biochemistry 1984;23:4075-81.
6. Laposata EA, Scherer DE, Lange LG. Fatty acid ethyl esters in adipose tissues. Arch Pathol Lab Med 1989;113:762-6.
7. Laposata EA, Harrison EH, Hedberg EB. Synthesis and degradation of fatty acid ethyl esters by cultured hepatoma cells exposed to ethanol. J Biol Chem 1990;265:9688-93.
8. DePergola G, Kjellstrom C, Holm C, Conradi N, Pettersson P, Bjorntorp P. The metabolism of ethyl esters of fatty acids in adipose tissue of rats chronically exposed to ethanol. Alcohol Clin Exp Res 1991;15:184-9.
9. Doyle KM, Bird DA. Al-Salihi S, Hallaq Y, Cluette-Brown JE, Goss KA, Laposata M. Fatty acid ethyl esters are present in human serum after ethanol ingestion. J Lipid Res 1994;35: 428-37.
10. Szczepiorkowski ZM, Dickerson GR, Laposata M. Fatty acid ethyl esters decrease human hepatoblastoma cell proliferation and protein synthesis. Gastroenterology 1995;108:515-22.
11. Shihabi ZK, Bishop C. Simplified turbidimetric assay for lipase activity. Clin Chem 1971;17:1150-3.
12. Pierre KJ, Tung KK, Nadj HA. A new enzymatic kinetic method for amylase. Clin Chem 1977;23:1369-70.
13. Bergmeyer HU, Hørder M, Rej R. International Federation of Clinical Chemistry (IFCC) scientific committee, analytical section: approved recommendation (1985) on IFCC methods for the measurement of catalytic concentration of enzymes. J Clin Chem Clin Biochem 1986;24:497-510.
14. Doumas BT, Perry BW, Sasse EA, Straumfjord JV Jr. Standardization in bilirubin assays: evaluation of selected methods and stability of bilirubin solutions. Clin Chem 1973;19:984-93.
15. 32P. J Clin Invest 1971;50:762-72.
16. Bernhardt TG, Cannistraro PA, Bird DA, Doyle KM, Laposata M. Purification of fatty acid ethyl esters by solid phase extraction and HPLC. J Chromat B: Biomed Appl 1995;in press.
17. Gorski NP, Novraldin H, Dube DM, Preffer FI, Dombkowski DM, Villa EM, et al. Reduced fatty acid ethyl ester synthase activity in the white blood cells of alcoholics. Alcoholism Clin Exp Res 1996; (in press).
18. Wright M, Bieser KJ, Kinnunen PM, Lange LG. Nonoxidative ethanol metabolism in human leukocytes: detection of fatty acid ethyl ester synthase activity. Biochem Biophys Res Commun 1987;142:979-85.