Small-scale production of Burkholderia cepacia ATCC21808 lipase adapted to high-throughput screening

Journal of Biomolecular Screening

Volumn 13, Issue 1, 2008, Pages 72-79

  Puech Guenot, Sophie ^a   Lafaquière, Vincent ^a   Guieysse, David ^a   Landric Burtin, Laure ^b   Monsan, Pierre ^a   Remaud Siméon, Magali ^a  

^a UMR792 INGÉNIERIE DES SYSTÈMES BIOLOGIQUES ET DES PROCÉDÉS   (France)

^b SANOFI   (France)

Author keywords

Burkholderia cepacia lipase; High throughput screening; Miniaturized production; Rational mutants; Recombinant expression in Escherichia coli

Indexed keywords

TRIACYLGLYCEROL LIPASE;

ARTICLE; BURKHOLDERIA CEPACIA; CULTURE MEDIUM; ENZYME SYNTHESIS; ERLENMEYER FLASK; HIGH THROUGHPUT SCREENING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS;

BASE SEQUENCE; BURKHOLDERIA CEPACIA; DNA PRIMERS; DRUG EVALUATION, PRECLINICAL; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; GENETIC VECTORS; LIPASE; MUTAGENESIS, SITE-DIRECTED; PLASMIDS; RECOMBINANT PROTEINS;

BURKHOLDERIA CEPACIA; ESCHERICHIA COLI;

EID: 37749014021     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057107311226     Document Type: Article

References (21)

1. Koga Y., Kato K., Nakano H., Yamane T.: Inverting enantioselectivity of Burkholderia cepacia KWI-56 lipase by combinatorial mutation and high-throughput screening using single-molecule PCR and in vitro expression. J Mol Biol 2003 ; 331: 585-592.
2. Kato R., Nakano H., Konishi H., Kato K., Koga Y., Yamane T.,, et al. Novel strategy for protein exploration: high-throughput screening assisted with fuzzy neural network. J Mol Biol 2005 ; 351: 683-692.
3. Reetz MT: Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications. Proc Natl Acad Sci U S A 2004 ; 101: 5716-5722.
4. Reetz MT, Carballeira JD, Peyralars J., Höbenreich H., Maichele A., Vogel A.: Expanding the substrate scope of enzymes: combining mutations obtained by CASTing. Chemistry 2006 ; 12: 6031-6038.
5. Reetz MT, Puls M., Carballeira JD, Vogel A., Jaeger KE, Eggert T.,, et al. Learning from directed evolution: furthers lessons from theoretical investigations into cooperative mutations in lipase enantioselectivity. Chembiochem 2007 ; 8: 106-112.
6. Tranel F., Haufe G.: Lipase-catalysed resolution of 2-fluorodecanoic acid. Tetrahedron Asymmetry 2000 ; 11: 889-896.
7. Kamal A., Sandbhor M., Ramana KV: One-pot lipase-catalyzed synthesis of enantiopure secondary alcohols from carbonyl compounds: a new protocol for lipase-mediated resolution. Tetrahedron Asymmetry 2002 ; 13: 815-820.
8. Guieysse D., Salagnad C., Monsan P., Remaud-Simeon M.: Lipase-catalysed enantioselective transesterification toward esters of 2-bromo-tolylacetic acids. Tetrahedron Asymmetry 2003 ; 14: 317-323.
9. Köhler J., Wünsch B.: Lipase-catalysed enantioselective desymmetrization of prochiral pentane-1,3,5-triol derivative. Tetrahedron Asymmetry 2006 ; 17: 3091-3099.
10. Shah S., Gupta MN: Kinetic resolution of (±)-1-phenylethanol in [Bmim] [PF6] using high activity preparations of lipases. Bioorg Med Chem Lett 2007 ; 17: 921-924.
11. Chojnacka A., Obara R., Wawrzenczyk C.: Kinetic resolution of racemic secondary aliphatic allylic alcohols in lipase-catalysed transesterification. Tetrahedron Asymmetry 2007 ; 18: 101-107.
12. Quyen DT, Schmidt-Dannert C., Schmid RD: High-level formation of active Pseudomonas cepacia lipase after heterologous expression of the encoding gene and its modified chaperone in Escherichia coli and rapid in vitro refolding. Appl Environ Microbiol 1999 ; 65: 787-794.
13. Rosenau F., Tommassen J., Jaeger KE: Lipase-specific foldases. Chembiochem 2004 ; 5: 152-161.
14. Guzman LM, Belin D., Carson MJ, Beckwith J.: Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J Bacteriol 1995 ; 177: 4121-4130.
15. Liu D., Schmid RD, Rusnak M.: Functional expression of Candida antarctica lipase B in the Escherichia coli cytoplasm-a screening system for a frequently used biocatalyst. Appl Microbiol Biotechnol 2006 ; 72: 1024-1032.
16. Pfeffer J., Rusnak M., Hansen CE, Bel Rhlid R., Schmid RD, Maurer SC: Functional expression of lipase A from Candida antarctica in Escherichia coli-a prerequisite for high-throughput screening and directed evolution. J Mol Catal B Enzym 2007 ; 45: 62-67.
17. Rosenau F., Jaeger KE: Bacterial lipases from Pseudomonas: regulation of gene expression and mechanisms of secretion. Biochimie 2000 ; 82: 1023-1032.
18. Ihara F., Okamoto I., Akao K., Nihira T., YamadaY: Lipase modulator protein (LimL) of Pseudomonas sp. strain 109. J Bacteriol 1995 ; 177: 1254-1258.
19. Traub PC, Schmidt-Dannert C., Schmitt J., Schmid RD: Gene synthesis, expression in E. coli, and in vitro refolding of Pseudomonas sp. KWI-56 and Chromobacterium visccosum lipases and theirs chaperones. Appl Microbiol Biotechnol 2001 ; 55: 198-204.
20. Bergstrom CP, Wagner CR, Ann DK, Hanna PE: Hamster monomorphic arylamine N-acetyltransferase: expression in Escherichia coli and purification. Protein Expr Purif 1995 ; 6: 45-55.
21. Guieysse D., Salagnad C., Monsan P., Remaud-Simeon M., Tran V.: Toward a novel explanation of Pseudomonas cepacia lipase enantioselectivity via molecular modelling of the enantiomer trajectory into the active site. Tetrahedron Asymmetry 2003 ; 14: 1807-1817.