Estimation of fungal growth using the ergosterol assay: A rapid tool in assessing the microbiological status of grains and feeds

Letters in Applied Microbiology

Volumn 46, Issue 1, 2008, Pages 113-118

  Ng, H E ^a   Raj, S S A ^a   Wong, S H ^a   Tey, D ^a   Tan, H M ^a  

^a Kemin Industries Inc   (United States)

Author keywords

Aspergillus niger ATCC 24126; Carbon dioxide production test; Ergosterol; Fungal contamination; Fungi; Mould inhibitor

Indexed keywords

ASPERGILLUS; GRAIN (AGRICULTURAL PRODUCT); MAMMALS; MOLDS; PELLETIZING;

ASPERGILLUS NIGER; ASPERGILLUS NIGER ATCC 24126; CARBON DIOXIDE PRODUCTION TEST; CORRELATION COEFFICIENT; ERGOSTEROL; FUNGAL CONTAMINATION; FUNGAL GROWTH; GOOD CORRELATIONS; MOLD INHIBITORS; PRODUCTION TEST;

CARBON DIOXIDE;

CARBON DIOXIDE; ERGOSTEROL;

ALCOHOLS; BIOASSAY; CORRELATION METHODS; FUNGI; MICROBIOLOGY;

ASSESSMENT METHOD; BIOASSAY; ESTIMATION METHOD; FOOD QUALITY; FUNGUS; GROWTH;

ACCURACY; ANIMAL FOOD; ARTICLE; ASPERGILLUS NIGER; CONTROLLED STUDY; CORN; FOOD CONTAMINATION; FOOD QUALITY; FUNGUS GROWTH; GRAIN; MOULD; NONHUMAN; QUANTITATIVE ANALYSIS;

ASPERGILLUS NIGER; FUNGI; SUIDAE;

EID: 37549068932     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2007.02279.x     Document Type: Article

References (20)

1. Abramson, D., Hulasare, R., White, N., Jayas, D. Marquardt, R. (1999) Mycotoxin formation in hullness barley during granary storage at 15% and 19% moisture content. J Stored Prod Res 35, 297 305.
2. Axelsson, B., Saraf, A. Larsson, L. (1995) Determination of ergosterol in organic dust by gas chromatography-mass spectrometry. J Chromatogr 666, 77 84.
3. Bailly, J., Le Bars, P., Pietri, A., Benard, G. Le Bars, J. (1999) Evaluation of a fluorodensitometric method for analysis of ergosterol as a fungal marker in compound feeds. J Food Prot 62, 686 690.
4. Donald, W. Mirocha, C. (1997) Chitin as a measure of fungal growth in stored corn and soybean seed. Cereal Chem 54, 466 474.
5. Jarvis, B., Seiler, D., Ould, A. Williams, A. (1983) Observations on the enumeration of moulds in food and feeding stuffs. J Appl Bacteriol 55, 325 336.
6. Kaspersson, A. (1986) The role of fungi in deterioration of stored feeds. PhD Thesis Report 31, Uppsala, Sweden : Department of Microbiology, Swedish University of Agricultural Sciences.
7. Lacey, I., Hills, S. Edwards, M. (1980) Micro-organisms in stored grains, their enumeration and significance. Trop Stored Prod Inf 39, 19 33.
8. Moretzsohn de Castro, M., Bragagnolo, N. Toledo Valentini, S. (2002) The relationship between fungi growth and aflatoxin production with ergosterol content of corn grains. Brazilian J Microbiol 33, 22 26.
9. Newell, S. (1992) Estimating fungal biomass and productivity in decomposing litter. In The Fungal Community, 2nd edn ed Carroll, G.C. Wicklow, D.T. pp. 521 561 New York, NY : Marcel Dekker, Inc.
10. Newell, S. (1994) Total and free ergosterol in mycelia of saltmarsh ascomycetes with access to whole leaves or aqueous extracts of leaves. Appl Environ Microbiol 60, 3479 3482.
11. Nout, M., Bonants-van Laarhoven, T., de Jongh, P. de Koster, P. (1987) Ergosterol content of Rhizopus oligosporous NRRL 5905 grown in liquid and solid substrates. Appl Microb Biotechnol 26, 456 461.
12. Pietri, A., Bertuzzi, T., Pallaroni, L. Piva, G. (2004) Occurrence of mycotoxins and ergosterol in maize harvested over 5 years in Northern Italy. Food Addit Contam 21, 479 487.
13. Saxena, J., Munimbazi, C. Bullerman, L. (2001) Relationship of mould count, ergosterol and ochratoxin A production. Int J Food Microbiol 71, 29 34.
14. Schnurer, J. Jonsson, A. (1992) Ergosterol levels and mould colony forming units in Swedish grains of food and feed grade. Acta Agric Scand 42, 240 245.
15. Seitz, L., Mohr, H., Burroughs, R Sauer, D. (1977) Ergosterol as an indicator of fungal invasion in grains. Cereal Chem 54, 1207 1217.
16. Seitz, L., Sauer, D., Burroughs, R., Mohr, H. Hubbard, J. (1979) Ergosterol as a measure of fungal growth. Phytopathology 69, 1202 1203.
17. Srzednicki, G., Craske, J., Nimmuntavin, C., Mantais, L.G. Wattananon, S. (2004) Determination of ergosterol in paddy rice using solid phase extraction. J Sci Food Agric 84, 2041 2046.
18. Tothill, I., Harris, D. Magan, N. (1992) The relationship between fungal growth and ergosterol content of wheat grain. Mycol Res 96, 965 970.
19. Weete, J. Gandhi, S. (1996) Biochemistry and molecular biology of fungal sterols. In The Mycota III Biochemistry and Molecular Biology ed. Brambl, R. Marzluf, G. pp. 421 438 Berlin, Germany : Springer.
20. Whipps, J. Lewis, D. (1980) Methodology of a chitin assay. Trans British Mycol Soc 79, 178 179.