Diastereoselective synthesis of (R)-(alkyl)-β-d-galactopyranoside by using β-galactosidase (Aspergillus oryzae) in low-water media

Bioorganic and Medicinal Chemistry Letters

Volumn 18, Issue 1, 2008, Pages 124-128

  Majumder, Abir B ^a   Singh, Bhupender ^a   Gupta, Munishwar N ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

Galactosidase; Diastereoselective synthesis; Galactoside synthesis; Glycoconjugates; Low water media; Reverse hydrolysis

Indexed keywords

1 PHENYLETHANOL; 1 PHENYLETHYL BETA DEXTRO GALACTOPYRANOSIDE; 2 OCTANOL; 2 OCTYL BETA DEXTRO GALACTOPYRANOSIDE; BETA GALACTOSIDASE; GALACTOSE; PROPANOL; UNCLASSIFIED DRUG; WATER;

ALPHA HELIX; ARTICLE; ASPERGILLUS ORYZAE; CHEMICAL STRUCTURE; DIASTEREOISOMER; ENANTIOSELECTIVITY; ENZYME ACTIVITY; FREEZE DRYING; INFRARED SPECTROSCOPY; POWDER; PRECIPITATION; RACEMIC MIXTURE; REACTION ANALYSIS; REACTION TIME; STEREOCHEMISTRY; STRUCTURE ANALYSIS; SYNTHESIS;

ASPERGILLUS ORYZAE; BETA-GALACTOSIDASE; FREEZE DRYING; GALACTOSIDES; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; STEREOISOMERISM; WATER;

ASPERGILLUS ORYZAE;

EID: 37549041698     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2007.11.006     Document Type: Article

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14. 3 frozen at -23 °C, and was lyophilized for 24 h. In another case, the same amount of enzyme was dissolved along with galactose (18.6 mg) and was lyophilized for 24 h.EPRP (enzyme precipitated and rinsed with propanol): 25 μL of enzyme solution (20 mg/100 μL of 100 mM phosphate buffer, pH 7.3) was precipitated in 200 μL of ice cold dry n-propanol, centrifuged, and the recovered pellet was repeatedly rinsed with n-propanol before using for the reaction.
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