Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTX-M genes in Enterobacteriaceae

APMIS

Volumn 115, Issue 12, 2007, Pages 1400-1408

  Monstein, H J ^a,b   Ostholm Balkhed A ^a   Nilsson, M V ^a   Nilsson, M ^a,b   Dornbusch, K ^a   Nilsson, L E ^a,b  

^a LINKÖPING UNIVERSITY HOSPITAL   (Sweden)

^b LINKÖPING UNIVERSITY   (Sweden)

Author keywords

blaCTX M genes; blaSHV; blaTEM genes; Automated DNA extraction; ESBL; GenomiPhi plasmid DNA; Multiplex PCR assay

Indexed keywords

EXTENDED SPECTRUM BETA LACTAMASE;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CITROBACTER FREUNDII; CONTROLLED STUDY; CTX M GENE; DNA SEQUENCE; ENTEROBACTER CLOACAE; ENTEROBACTERIACEAE; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE IDENTIFICATION; GENE LOCATION; GRAM NEGATIVE BACTERIUM; HUMAN; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; MOLECULAR TYPING; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SHV GENE; TEM GENE;

AMINO ACID SEQUENCE; BETA-LACTAMASES; CLONING, MOLECULAR; DNA PRIMERS; DNA, BACTERIAL; ENTEROBACTERIACEAE; HUMANS; MOLECULAR SEQUENCE DATA; PHENOTYPE; POLYMERASE CHAIN REACTION;

EID: 37549033874     PISSN: 09034641     EISSN: 16000463     Source Type: Journal    
DOI: 10.1111/j.1600-0463.2007.00722.x     Document Type: Article

References (24)

1. Ambler RP, Coulson AFW, Frére JM, Ghuysen JM, Joris B, Forsman M, et al. A standard numbering scheme for the class a β-lactamases. Biochem J 1991 276: 269 72.
2. Shah AA, Hasan F, Ahmed S, Hameed A. Extended - spectrum β-lactamases (ESBLs): characterization, epidemiology and detection. Crit Rev Microbiol 2004 30: 25 32.
3. Bradford PA. Extended - spectrum β-lactamases in the 21st Century: characterisation, epidemiology, and detection of this important resistance threat. Clin Microbiol Rev 2001 14: 933 51.
4. Bonnet R. Growing group of extended - spectrum β-lactamases: the CTX-M enzymes. Antimicrob Agents Chemother 2004 48: 1 14.
5. Verma A, Desai N, Shannon K, Philpott-Howard J, Hill RLR. Intra- and inter - generic plasmid - mediated spread of cephalosporin and aminoglycoside resistance amongst Klebsiella aerogenes K41 and other Enterobacter. Int J Antimicrob Agents 2001 17: 123 9.
6. Yan JJ, Ko WCh, Wu JJ. Identification of a plasmid encoding SHV-12, TEM-1, and a variant of IMP-s metallo-β-lactamase, IMP-8, from a clinical isolate of Klebsiella pneumonia. Antimicrob Agent Chemother 2001 45: 2368 71.
7. Dean FB, Nelson JR, Giesler TL, Lasken RS. Rapid amplification of plasmid and phage DNA using Phi29 DNA polymerase and multiply-primed rolling circle amplification. Genome Res 2001 11: 1095 9.
8. Nelson JR, Cai YC, Giesler TL, Farchaus JW, Sundaram ST, Ortiz-Rivera M, et al. TempliPhi 29 DNA polymerase based rolling circle amplification of templates for DNA sequencing. Biotechniques 2002 32: S44 S47.
9. Reagin MJ, Giesler TL, Merla AL, Resetar-Gerke JM, Kapolka KM, Mamone JA. TempliPhi: a sequencing template procedure that eliminates overnight cultures and DNA purification. J Biomol Techniques 2003 14: 143 8.
10. Hernandez JR, Martinez-Martinez L, Canton R, Coque TM, Pasqual A the Spanish Group for Nosocomial Infections (GEIH). Nationwide study of Escherichia coli and Klebsiella pneumonia producing extended-spectrum-β-lactamases in Spain. Antimicrob Agents Chemother 2005 49: 2122 5.
11. Morris D, O'Hare C, Glennon M, Maher M, Corbett-Feeney G, Cormican M. Extended-spectrum β-lactamase in Ireland, including a novel enzyme, TEM-102. Antimicrob Agent Chemother 2003 47: 2572 8.
12. Paterson DL, Hujer KM, Hujer AM, Yeiser B, Bonomo MD, Rice LB, Bonomo RA the International Klebsiella study group. Extended-spectrum β-lactamases in Klebsiella pneumoniae bloodstream isolates from seven countries: dominance and widespread prevalence of SHV-and CTX-M-type β-lactamases. Antimicrob Agents Chemother 2003 47: 3553 60.
13. Quinteros M, Radice M, Gardella N, Rodriguez MM, Costa N, Korbenfeld D, Couto E, Gutkind G the microbiology study Group. Extended-spectrum β-lactamases in Enterobacteriaceae in Buenos Aires, Argentina, public health Hospitals. Antimicrob Agents Chemother 2003 47: 2864 7.
14. Boyd DA, Tyler S, Christianson S, McGeer A, Muller MP, Willey BM, Bryce E, Gardam M, Nordmann P, Mulvey MR the Canadian nosocomial infection surveillance program, Health Canada. Complete nucleotide sequence of a 92-kilobase plasmid harbouring the CTX-M-15 extended-spectrum beta-lactamase involved in an outbreak in long-term-care facilities in Toronto, Canada. Antimicrob Agents Chemother 2004 48: 3758 64.
15. Colom K, Pérez J, Alonso R, Fernández-Aranguiz A, Larino E, Cisterna R. Simple and reliable multiplex PCR assay for the detection of blaTEM, blaSHV, and blaOXA-1 genes in Enterobacteriaceae. FEMS Microbiol Lett 2003 223: 147 51.
16. Chia JH, Chu C, Su LH, Chiu CH, Kuo AJ, Sun CF, et al. Development of a multiplex PCR and SHV melting-curve mutation detection system for detection of some SHV and CTX-M β-lactamases of Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae in Taiwan. J Clin Microbiol 2005 43: 4486 91.
17. Woodford N, Fagan EJ, Ellington MJ. Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrum (beta)-lactamases. J Antimicrob Chemother 2006 57: 154 5.
18. Potz NAC, Colman M, Warner M, Reynolds R, Livermore DM. False-positive extended-spectrum beta-lactamase tests for Klebsiella oxytoca strains hyperproducing K1 beta-lactamase. J Antimicrob Chemother 2004 53: 545 7.
19. Wu SW, Dornbusch K, Kronvall G, Norgren M. Characterization and nucleotide sequence of a Klebsiella oxytoca cryptic plasmid encoding a CMY-type beta-lactamase: confirmation that the plasmid-mediated cephamycinase originated from the Citrobacter freundii AmpC beta-lactamase. Antimicrob Agents Chemother 1999 43: 1350 7.
20. Haeggman S, Löfdahl S, Burman LG. An allelic variant of the chromosomal gene for class A beta-lactamase K2. specific for Klebsiella pneumoniae, is the ancestor of SHV-1. Antimicrob Agents Chemother 1997 41: 2705 9.
21. Chaves J, Ladona MG, Segura C, Coira A, Reig R, Ampurdanés C. SHV-1 β-lactamase is mainly a chromosomally encoded species-specific enzyme in Klebsiella pneumoniae. Antimicrob Agents Chemother 2001 45: 2856 61.
22. Haeggman S, Löfdahl S, Paauw A, Verhoef J, Brisse S. Diversity and evolution of the class A chromosomal beta-lactamase gene in Klebsiella pneumoniae. Antimicrob Agents Chemother 2004 48: 2400 8.
23. Eckert C, Gautier V, Arlet G. DNA sequence analysis of the genetic environment of various bla-CTX-M genes. J Antimicrob Chemother 2006 57: 14 23.
24. Rodriguez-Villalobos H, Malaviolle V, Frankard J, De Mendonca R, Nonhoff C, Struelens MJ. In vitro activity of temocillin against extended spectrum β-lactamase-producing Escherichia coli. J Antimicrob Chemother 2006 57: 771 4.