Simple and reliable multiplex PCR assay for detection of blaTEM, blaSHV and blaOXA-1 genes in Enterobacteriaceae

FEMS Microbiology Letters

Volumn 223, Issue 2, 2003, Pages 147-151

  Colom, Karmele ^a   Pérez, Javier ^a   Alonso, Rodrigo ^b   Fernández Aranguiz, Agueda ^a   Lariño, Eva ^a   Cisterna, Ramón ^a,b  

^a UNIVERSITY OF THE BASQUE COUNTRY UPV EHU   (Spain)

^b HOSPITAL DE BASURTO   (Spain)

Author keywords

BlaOXA 1; BlaSHV; BlaTEM; Enterobacteriaceae; Multiplex polymerase chain reaction

Indexed keywords

AMOXICILLIN; BACTERIAL PROTEIN; CLAVULANIC ACID;

AMPLICON; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CONTROLLED STUDY; ENTEROBACTERIACEAE; ESCHERICHIA COLI; GENETIC ANALYSIS; GENETIC ASSOCIATION; GENETIC SCREENING; HYBRIDIZATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ENTEROBACTERIACEAE; ESCHERICHIA COLI;

EID: 0038771950     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00306-9     Document Type: Article

References (16)

1. Thomson K., Moland E.S. Version 2000: the new β-lactamases of gram-negative bacteria at the dawn of the new millennium. Microb. Infect. 2:2000;1225-1235.
2. Chaibi E.B., Sirot D., Paul G., Labia R. Inhibitor-resistant TEM β-lactamases: phenotypic, genetic and biochemical characteristics. J. Antimicrob. Chemother. 43:1999;447-458.
3. Bergström S., Normark S. β-Lactam resistance in clinical isolates of Escherichia coli caused by elevated production of the ampC-mediated chromosomal β-lactamase. Antimicrob. Agents Chemother. 16:1979;427-433.
4. Shannon K., Williams H., King A., Philipps I. Hyperproduction of TEM-1 β-lactamase in clinical isolates of Escherichia coli serotype O15. FEMS Microbiol. Lett. 67:1990;319-324.
5. Zhou X.Y., Bordon F., Sirot D., Kitzis M.D., Gutmann L. Emergence of clinical isolates of Escherichia coli producing TEM-1 derivatives or an OXA-1 β-lactamase conferring resistance to β-lactamase inhibitors. Antimicrob. Agents Chemother. 38:1994;1085-1089.
6. Prinarakis E.E., Miriagou V., Tzelepi E., Gazouli M., Tzouvelekis L.S. Emeregence of an inhibitor-resistant β-lactamase (SHV-10) derived from an SHV-5 variant. Antimicrob. Agents Chemother. 41:1997;838-840.
7. Livermore, D.M. and Williams J.D. (1996) β-Lactams: mode of action and mechanisms of bacterial resistance. In: Antibiotics in Laboratory Medicine (Lorian, V., Ed.), pp. 502-78. Williams and Wilkins, Baltimore, MD.
8. Mabilat, C. and Goussard S. (1993) PCR detection and identification of genes for extended-spectrum β-lactamases. In: Diagnostic Molecular Microbiology: Principles and Applications (Persing, D.H., Smith, T.F., Tenover, F.C. and White, T.J., Eds.), pp. 553-559. American Society for Microbiology, Washington, DC.
9. British Society for Antimicrobial Chemotherapy (2001) Disc diffusion method for antimicrobial susceptibility testing.
10. Mabilat C., Courvalin P. Development of oligotyping for characterization and molecular epidemiology of TEM β-lactamases in members of the family Enterobacteriaceae. Antimicrob. Agents Chemother. 34:1990;2210-2216.
11. Speldooren V., Heym B., Labia R., Nicolas-Chanoine M-H. Discriminatory detection of inhibitor-resistant β-lactamases in Escherichia coli by single-strand conformation polymorphism-PCR. Antimicrob. Agents Chemother. 42:1998;879-884.
12. Nicolas-Chanoine M-H., Jarlier V., Honore N., Philippon A., Cole S.T. Molecular characterization of the gene encoding SHV-3 β-lactamase responsible for transferable cefotaxime resistance in clinical isolates of Klebsiella pneumoniae. Antimicrob. Agents Chemother. 33:1989;2096-2100.
13. Ouellette M., Bissonnette L., Roy P.H. Precise insertion of antibiotic resistance determinants into TN21-like transposons: nucleotide sequence of the OXA-1 β-lactamase gene. Proc. Natl. Acad. Sci. USA. 84:1987;7378-7382.
14. Elnifro E.M., Ashshi A.M., Cooper R.J., Klapper P.E. Multiplex PCR: Optimization and application in diagnostic virology. Clin. Microbiol. Rev. 13:2000;559-570.
15. Henquell C., Chanal C., Sirot D., Labia R., Sirot J. Molecular characterization of nine different types of mutants among 107 inhibitor-resistant TEM beta-lactamases from clinical isolates of Escherichia coli. Antimicrob. Agents Chemother. 34:1995;707-714.
16. Livermore D.M., Brown D.F.J. Detection of β-lactamase-mediated resistance. J. Antimicrob Chemother. 48:(Suppl. S1):2001;59-64.