TempliPhi, φ29 DNA polymerase based rolling circle amplification of templates for DNA sequencing

BioTechniques

Volumn 32, Issue 6 SUPPL., 2002, Pages

  Nelson, John R ^a   Cai, Yuyang Christine ^a   Giesler, Theresa L ^a   Farchaus, Joseph W ^a   Sundaram, Shanmuuga T ^a   Ortiz Rivera, Maria ^a   Hosta, Lou P ^a   Hewitt, Peter L ^a   Mamone, J Anthony ^a   Palaniappan, C ^a   Fuller, Carl W ^a  

^a Amersham Biosciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; DNA SEQUENCES; ENZYMES;

BACTERIAL COLONY;

GENETIC ENGINEERING;

DNA POLYMERASE; DOUBLE STRANDED DNA; BACTERIAL DNA; CIRCULAR DNA; DIAGNOSTIC AGENT; DNA DIRECTED DNA POLYMERASE; VIRUS DNA;

ARTICLE; ASSAY; BACTERIOPHAGE; BACTERIUM COLONY; DNA SEQUENCE; DNA TEMPLATE; GENE AMPLIFICATION; GENOTYPE; NONHUMAN; BACILLUS PHAGE; ESCHERICHIA COLI; EVALUATION; GENE LIBRARY; GENETICS; HUMAN; INSTRUMENTATION; METHODOLOGY; NUCLEIC ACID AMPLIFICATION; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; VIROLOGY;

BACTERIA (MICROORGANISMS);

BACILLUS PHAGES; DNA, BACTERIAL; DNA, CIRCULAR; DNA, VIRAL; DNA-DIRECTED DNA POLYMERASE; ESCHERICHIA COLI; GENE LIBRARY; HUMANS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; TEMPLATES, GENETIC;

EID: 0035990827     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/jun0208     Document Type: Article

References (7)

1. Highly efficient DNA synthesis by the phage phi 29 DNA polymerase. Symmetrical mode of DNA replication
2. Relating structure to function in phi29 DNA polymerase
3. Rapid amplification of plasmid and phage DNA using phi29 DNA polymerase and multiply-primed rolling circle amplification
4. Errors in the polymerase chain reaction
5. Fidelity of phi 29 DNA polymerase. Comparison between protein-primed initiation and DNA polymerization
6. The mutational specificity of DNA polymerase-beta during in vitro DNA synthesis. Production of frameshift, base substitution, and deletion mutations
7. Mutation detection and single-molecule counting using isothermal rolling-circle amplification