High-level expression and purification of Tat-haFGF19-154

Applied Microbiology and Biotechnology

Volumn 77, Issue 5, 2008, Pages 1015-1022

  Huang, Yadong ^a   Rao, Yulan ^b,c   Feng, Chengli ^d   Li, Yanmei ^a   Wu, Xiaoping ^a   Su, Zhijian ^a   Xiao, Jian ^b   Xiao, Yechen ^e   Feng, Wenke ^f   Li, Xiaokun ^b,e  

^a JINAN UNIVERSITY   (China)

^b WENZHOU MEDICAL UNIVERSITY   (China)

^c CHINA PHARMACEUTICAL UNIVERSITY   (China)

^d SHAANXI INSTITUTE OF ZOOLOGY   (China)

^e JILIN AGRICULTURAL UNIVERSITY   (China)

^f University of Louisville School of Medicine   (United States)

Author keywords

Acidic fibroblast growth factor; Expression; Mitogenic activity; Purification; Tat PTD

Indexed keywords

ACIDIC FIBROBLAST GROWTH FACTOR; GEL ELECTROPHORESIS; MITOGENIC ACTIVITY; POLYMERASE CHAIN REACTION;

AFFINITY CHROMATOGRAPHY; BIOASSAY; ELECTROPHORESIS; ESCHERICHIA COLI; FIBROBLASTS; PROTEINS;

GENE EXPRESSION;

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; CELL PROTEIN; HEPARIN; HISTIDINE; HYBRID PROTEIN; RECOMBINANT PROTEIN; SEPHADEX; TRANSACTIVATOR PROTEIN PROLYLTHREONYLASPARTIC ACID[49-57] ACIDIC FIBROBLAST GROWTH FACTOR[19-154] HISTIDINE FUSION PROTEIN; UNCLASSIFIED DRUG;

ASSAY; BLOOD; CHROMATOGRAPHY; COLIFORM BACTERIUM; DETECTION METHOD; ELECTROKINESIS; EXPERIMENTAL STUDY; GENE EXPRESSION; INJURY; NERVOUS SYSTEM; POLYMERASE CHAIN REACTION; PROTEIN; RECOMBINATION;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BACTERIAL CELL; BACTERIUM CULTURE; BAGG ALBINO MOUSE; BRAIN CORTEX; CELL ASSAY; CELL MEMBRANE PERMEABILITY; CELL STRAIN 3T3; CONTROLLED STUDY; DRUG PENETRATION; DRUG PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE AMPLIFICATION; GENETIC TRANSDUCTION; HIPPOCAMPUS; MITOGENESIS; MOUSE; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION;

ANIMALS; BALB 3T3 CELLS; CELL PROLIFERATION; CEREBRAL CORTEX; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FIBROBLAST GROWTH FACTORS; GENE EXPRESSION; HIPPOCAMPUS; HUMANS; MICE; RECOMBINANT FUSION PROTEINS; TAT GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS;

ESCHERICHIA COLI;

EID: 37449009848     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-1249-5     Document Type: Article

References (40)

1. Alexi T, Borlongan CV, Faull RL, Williams CE, Clark RG, Gluckman PD, Hughes PE (2000) Neuroprotective strategies for basal ganglia degeneration: Parkinson's and Huntington's diseases. Prog Neurobiol 60:409-470
2. Bannerman PG, Oliver TM, Xu Z, Shieh DE (1996) Pleasure, Effects of FGF-1 and FGF-2 on GD3 immunoreactive spinal neuroepithelial cells. J Neurosci Res 45:549-557
3. Bayley H (1999) Protein therapy-delivery guaranteed. Nat Biotechnol 17:1066-1067
4. Cao G, Pei W, Ge H, Liang Q, Luo Y, Sharp FR, Lu A, Ran R, Graham SH, Chen J (2002) In vivo delivery of a Bcl-xL fusion protein containing the TAT protein transduction domain protects against ischemic brain injury and neuronal apoptosis. J Neurosci 22:5423-5431
5. Cuevas P, Carceller F, Lozano RM, Crespo A, Zazo M, Gimenez-Gallego G (1997) Protection of rat myocardium by mitogenic and non-mitogenic fibroblast growth factor during post-ischemic reperfusion. Growth Factors 15:29-40
6. Cuevas P, Carceller F, Munoz-Willery I, Gimenez-Gallego G (1998) Intravenous fibroblast growth factor penetrates the blood-brain barrier and protects hippocampal neurons against ischemia-reperfusion injury. Surg Neurol 49:77-83
7. Cuevas P, Gimenez-Gallego G (1996) Antiepileptic effects of acidic fibroblast growth factor examined in kainic acid-mediated seizures in the rat. Neurosci Lett 203:66-68
8. Cuevas P, Revilla C, Herreras O, Largo C, Gimenez-Gallego G (1994) Neuroprotective effect of acidic fibroblast growth factor on seizure-associated brain damage. Neurol Res 16:365-369
9. Date I, Notter MF, Felten SY, Felten DL (1990) MPTP- treated young mice but not aging mice show partial recovery of the nigrostriatal dopaminergic system by stereotaxic injection of acidic fibroblast growth factor (aFGF). Brain Res 526:156-160
10. Dietz GPH, Kilic E, Bähr M (2002) Inhibition of neuronal apoptosis in vitro and in vivo using TAT-mediated protein transduction. Mol Cell Neurosci 21:29-37
11. Dietz GPH, Valbuena PC, Dietz B, Meuer K, Müller P, Weishaupt JH, Bähr M (2006) Application of a blood-brain-barrier-penetrating form of GDNF in a mouse model for Parkinson's disease. Brain Res 1082:61-66
12. Elliott G, O'Hare P (1997) Intercellular trafficking and protein delivery by a herpes virus structural protein. Cell 88:223-233
13. Emerich DF, Dean RL, Osborn C, Bartus RT (2001) The development of the bradykinin agonist labradimil as a means to increase the permeability of the blood-brain barrier: from concept to clinical evaluation. Clin Pharmacokinet 40:105-123
14. Engele J, Bohn MC (1992) Effects of acidic and basic fibroblast growth factors on glial precursor cell proliferation: age dependency and brain region specificity. Dev Biol 152:363-372
15. Federoff HJ, Geschwind MD, Geller AI, Kessler JA (1992) Expression of nerve growth factor in vivo from a defective herpes simplex virus 1 vector prevents effects of axotomy on sympathetic ganglia. Proc Natl Acad Sci USA 89:1636-1640
16. Figueiredo BC, Pluss K, Skup M, Otten U, Cuello AC (1995) Acidic FGF induces NGF and its mRNA in the injured neocortex of adult animals. Mol Brain Res 33:1-6
17. Gimenez-Gallego G, Cuevas P (1994) Fibroblast growth factors, proteins with a broad spectrum of biological activities. Neurol Res 16:313-316
18. Imamura T, Engleka K, Zhan X, Tokita Y, Forough R, Roeder D, Jackson A, Maier JA, Hla T, Maciag T (1990) Recovery of mitogenic activity of a growth factor mutant with a nuclear translocation sequence. Science 249:1567-1570
19. Jankowsky JL, Patterson PH (2001) The role of cytokines and growth factors in seizures and their sequelae. Prog Neurobiol 63:125-149
20. Kilic Ü, Kilic E, Dietz GPH, Bähr M (2003) Intravenous TAT-GDNF is protective after focal cerebral ischemia in mice. Stroke 34:1304-1310
21. Kim DW, Eum WS, Jang SH, Kim SY, Choi HS, Choi SH, An JJ, Lee SH, Lee KS, Han K, Kang TC, Won MH, Kang JH, Kwon OS, Cho SW, Kim TY, Park J, Choi SY (2005) Transduced TAT-SOD fusion protein protects against ischemic brain injury. Mol Cells 19:88-96
22. Klingenberg O, Wiedlocha A, Olsnes S (2000) Requirement of PI 3-kinase activity for translocation of exogenous aFGF both to cytosol and nucleus. J Biol Chem 275:11972-11980
23. Klingenberg O, Wiedlocha A, Rapak A, Munoz R, Falnes PO, Olsnes S (1998) Inability of the acidic fibroblast growth factor mutant K132E to stimulate DNA synthesis after translocation into cells. J Biol Chem 273:11164-11172
24. Li AJ, Oomura QY, Sasaki K, Suzuki K, Tooyama I, Hanai K, Kimura H, Hori T (1998) A single pre-training glucose injection induces memory facilitation in rodents performing various tasks: contribution of acidic fibroblast growth factor. Neuroscience 85:785-794
25. Lin YZ, Yao SY, Hawiger J (1996) Role of the nuclear localization sequence in fibroblast growth factor-1- stimulated mitogenic pathways. J Biol Chem 271:5305-5308
26. Lipton SA, Wangner JA, Maison RD, D'Amore PA (1988) Acidic fibroblast growth factor enhances regeneration of processes by postnatal mammalian retinal ganglion cells in culture. Proc Natl Acad Sci USA 85:2388-2392
27. 1H NMR structural characterization of a nonmitogenic, vasodilatory, ischemia-protector and neuromodulatory acidic fibroblast growth factor. Biochem 39:4982-4993
28. Luo Y, Gabriel JL, Wang F, Zhan X, Maciag T, Kan M, McKeehan WL (1996) Molecular modeling and deletion mutagenesis implicate the nuclear translocation sequence in structural integrity of fibroblast growth factor-1. J Biol Chem 271:26876-26883
29. Mufson EJ, Kroin JS, Sendera TJ, Sobreviela T (1999) Distribution and retrograde transport of trophic factors in the central nervous system: functional implications for the treatment of neurodegenerative diseases. Prog Neurobiol 57:451-484
30. Rapoport SI (2000) Osmotic opening of the blood-brain barrier: principles mechanism and therapeutic applications. Cell Mol Neurobiol 20:217-230
31. Sasaki K, Oomura Y, Suzuki K, Hanai K, Yagi H (1992) Acidic fibroblast growth factor prevents death of hippocampal CA1 pyramidal cells following ischemia. Neurochem Int 21:397-402
32. Schwarze SR, Ho A, Vocero-Akbani A, Dowdy SF (1999) In vivo protein transduction: delivery of a biologically active protein into the mouse. Science 285:1569-1572
33. Sengoku T, Bondada V, Hassane D, Dubal S, Geddes JW (2004) Tat-calpastatin fusion proteins transduce primary rat cortical neurons but do not inhibit cellular calpain activity. Exp Neurol 188:161-170
34. Wiedlocha A, Falnes PO, Rapak A, Munoz R, Klingenberg O, Olsnes S (1996) Stimulation of Proliferation of a human osteosarcoma cell line by exogenous acidic fibroblast growth factor requires both activation of receptor tyrosine kinase and growth factor internalization. Mol Cell Biol 16:270-280
35. Wilcox BJ, Unnerstall JR (1991) Expression of acidic fibroblast growth factor mRNA in the developing and adult rat brain. Neuron 6:397-409
36. Wu SP, Fu AL, Wang YX, Yu LP, Jia PY, Li Q, Jin GZ, Sun MJ (2006) A novel therapeutic approach to 6-OHDA-induced Parkinson's disease in rats via supplementation of PTD-conjugated tyrosine hydroxylase. Biochem Biophys Res Commun 346:1-6
37. Wu X, Su Z, Li X, Zheng Q, Huang Y, Yuan H (2005) High-level expression and purification of a nonmitogenic form of human acidic fibroblast growth factor in Escherichia coli. Protein Expr Purif 42:7-11
38. Wu X, Su Z, Zheng Q, Huang Y, Li X (2004) Expression and activity identification of shortened human acidic fibroblast growth factor. Journal of China Pharmaceutical University 35:470-473
39. Yin W, Cao G, Johnnides MJ, Signore AP, Luo Y, Hickey RW, Chen J (2006) TAT-mediated delivery of Bcl-xL protein is neuroprotective against neonatal hypoxic-ischemic brain injury via inhibition of caspases and AIF. Neurobiol Dis 21:358-371
40. Zalecki P, Radzikowski C, Olsnes S, Wiedlocha A (1998) Modulation by interleukin-2 of cellular response to fibroblast growth factor-1 in F69-3 fibrosarcoma cells. Exp Cell Res 244:61-70