A multiplex PCR that discriminates between Trypanosoma brucei brucei and zoonotic T. b. rhodesiense

Experimental Parasitology

Volumn 118, Issue 1, 2008, Pages 41-46

  Picozzi, Kim ^a   Carrington, Mark ^b   Welburn, Susan C ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Diagnosis; DNA; Epidemiology; Human African sleeping sickness; Multiplex; PCR; Phospholipase C, GPI PLC; Polymerase chain reaction; Serum Resistance Associated; SRA; T. b. rhodesiense; Trypanosomiasis; Whatman FTA; Zoonoses

Indexed keywords

ARTICLE; DNA EXTRACTION; GENE IDENTIFICATION; HUMAN; INFECTION RISK; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITOSIS; PRIORITY JOURNAL; TRYPANOSOMA BRUCEI; TRYPANOSOMA RHODESIENSE; TRYPANOSOMIASIS; ZOONOSIS;

ANIMALS; ANIMALS, DOMESTIC; ANIMALS, WILD; CATTLE; GLYCOSYLPHOSPHATIDYLINOSITOL DIACYLGLYCEROL-LYASE; HUMANS; MEMBRANE GLYCOPROTEINS; POLYMERASE CHAIN REACTION; PROTOZOAN PROTEINS; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; TRYPANOSOMA BRUCEI BRUCEI; TRYPANOSOMA BRUCEI RHODESIENSE; TRYPANOSOMIASIS, AFRICAN; VARIANT SURFACE GLYCOPROTEINS, TRYPANOSOMA; ZOONOSES;

ANIMALIA; TRYPANOSOMA BRUCEI; TRYPANOSOMA BRUCEI BRUCEI;

EID: 37349048539     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2007.05.014     Document Type: Article

References (42)

1. Artama W.T., Agey M.W., and Donelson J.E. DNA comparisons of Trypanosoma evansi (Indonesia) and Trypanosoma brucei spp. Parasitology 104 (1992) 67-74
2. Becker S., Franco J.R., Simarro P.P., Stich A., Abel P.M., and Steverding D. Real-time PCR for detection of Trypanosoma brucei in human blood samples. Diagnostic Microbiology and Infectious Disease 50 (2004) 193-199
3. Boid R., Jones T.W., and Payne R.C. Malic enzyme type VII isoenzyme as an indicator of suramin resistance in Trypanosoma evansi. Experimental Parasitology 69 (1989) 317-323
4. Borst P., Van der Ploeg M., Van Hoek J.F.M., Tas J., and James J. On the DNA content and Ploidy of Trypansomoes. Molecular and Biochemical Parasitology 6 (1982) 13-23
5. Campillo N., and Carrington M. The origin of the serum resistance associated (SRA) gene and a model of the structure of the SRA polypeptide from Trypanosoma brucei rhodesiense. Molecular and Biochemical Parasitology 127 (2003) 79-84
6. Carrington M., Bulow R., Reinke H., and Overath P. Sequence and expression of the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei. Molecular and Biochemical Parasitology 33 (1989) 289-296
7. Contamin H., Fandeur T., Bonnefoy S., Skouri F., Ntoumi F., and Mercereau-Puijalon O. PCR typing of field isolates of Plasmodium falciparum. Journal of Clinical Microbiology 33 (1995) 944-951
8. Cox A., Tilley A., McOdimba F., Fyfe J., Eisler M.C., Hide G., and Welburn S.C. A PCR based assay for detection and differentiation of African trypanosome species in blood. Experimental Parasitology 111 (2005) 24-29
9. De Greef C., Chimfwembe E., Wabacha J.K., Songa E.B., and Hamers R. Only the serum-resistant blood-stream forms of Trypanosoma brucei rhodesiense express the Serum Resistance Associated (SRA) protein. Annales De La Societe Belge De Medecine Tropicale 72 (1992) 13-21
10. De Greef C., Imberechts H., Matthyssens G., Vanmeirvenne N., and Hamers R. A gene expressed only in serum-resistant variants of Trypanosoma brucei rhodesiense. Molecular and Biochemical Parasitology 36 (1989) 169-176
11. Desquesnes M., McLaughlin G., Zoungrana A., and Davila A.M.R. Detection and identification of Trypanosoma of African livestock through a single PCR based on internal transcribed spacer 1 of rDNA. International Journal for Parasitology 31 (2001) 610-614
12. Gibson W., Backhouse T., and Griffiths A. The human serum resistance associated gene is ubiquitous and conserved in Trypanosoma brucei rhodesiense throughout East Africa. Infection, Genetics and Evolution 1 (2002) 207-214
13. Ginzinger D.G. Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Experimental Hematology 30 (2002) 503-512
14. Hereld D., Hart G.W., and Englund P.T. cDNA encoding the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei. Proceeding of the National Academy of Science, USA 85 (1988) 14-18
15. Hide G., Welburn S.C., Tait A., and Maudlin I. Epidemiological relationship of Trypanosoma brucei stocks from South East Uganda: evidence for different population structures in human infective and non-human infective isolates. Parasitology 109 (1994) 95-111
16. Jannotti-Passos L.K., Vidigal T.H., Dias-Neto E., Pena S.D., Simpson A.J., Dutra W.O., Souza C.P., and Carvalho-Parra J.F. PCR amplification of the mitochondrial DNA minisatellite region to detect Schistosoma mansoni infection in Biomphalaria glabrata snails. Journal of Parasitology 83 (1997) 395-399
17. Kaare M.T., Picozzi K., Mlengeya T., Fèvre E.M., Mtambo M.M., Mellau L.S., Cleaveland S., and Welburn S.C. Sleeping sickness-a re-emerging disease in the Serengeti?. Travel Medicine and Infectious Disease 5 (2007) 117-124
18. Kanmogne G.D., Asonganyi T., and Gibson W.C. Detection of Trypanosoma brucei gambiense, in serologically positive but aparasitaemic sleeping-sickness suspects in Cameroon, by PCR. Annals of Tropical Medicine and Parasitology 90 (1996) 475-483
19. Katunguka-Rwakishaya E. The prevalence of trypanosomosis in small ruminants and pigs in a sleeping sickness endemic area of Buikwe County, Mukono district, Uganda. Revue d Elevage et de Medecine Veterinaire des Pays Tropicaux 49 (1996) 56-58
20. Leeflang P., Buys J., and Blotkamp C. Studies on Trypanosoma vivax: infectivity and serial maintenance of natural bovine isolates in mice. International Journal for Parasitology 6 (1976) 413-417
21. MacLeod A., Turner C.M.R., et al. Detection of single copy gene sequences from single trypanosomes. Molecular and Biochemical Parasitology 84 2 (1997) 267-270
22. Masake R.A., Njuguna J.T., Brown C.C., and Majiwa P.A.O. The application of PCR-ELISA to the detection of Trypanosoma brucei and T. vivax infections in livestock. Veterinary Parasitology 105 (2002) 179-189
23. Mensa-Wilmot K., Hereld D., and Englund P.T. Genomic organization, chromosomal localization, and developmentally regulated expression of the glycosyl-phosphatidylinositol-specific phospholipase C of Trypanosoma brucei. Molecular and Cellular Biology 10 (1990) 720-726
24. Milner J.D., and Hajduk S.L. Expression and localization of serum resistance associated protein in Trypanosoma brucei rhodesiense. Molecular and Biochemical Parasitology 104 (1999) 271-283
25. Moser D.R., Cook G.A., Ochs D.E., Bailey C.P., McKane M.R., and Donelson J.E. Detection of Trypanosoma congolense and Trypanosoma brucei subspecies by DNA amplification using the polymerase chain reaction. Parasitology 99 (1989) 57-66
26. Njiru Z.K., Constantine C.C., Guya S., Crowther J., Kiragu J.M., Thompson R.C., and Davila A.M. The use of ITS1 rDNA PCR in detecting pathogenic African trypanosomes. Parasitology Research (2004)
27. Orlandi P.A., Carter L., Brinker A.M., da Silva A.J., Chu D.M., Lampel K.A., and Monday S.R. Targeting single-nucleotide polymorphisms in the 18S rRNA gene to differentiate Cyclospora species from Eimeria species by multiplex PCR. Applied and Environmental Microbiology 69 (2003) 4806-4813
28. Padley D., Moody A.H., Chiodini P.L., and Saldanha J. Use of a rapid, single-round, multiplex PCR to detect malarial parasites and identify the species present. Annals of Tropical Medicine and Parasitology 97 (2003) 131-137
29. Picozzi K., Tilley A., Fèvre E.M., Coleman P.G., Odiit M., Magona J., Eisler M.C., and Welburn S.C. The diagnosis of trypanosome infections: applications of novel technology for reducing disease risk. African Journal of Biotechnology 1 2 (2003) 39-45
30. Picozzi K., Fevre E.M., Odiit M., Carrington M., Eisler M.C., Maudlin I., and Welburn S.C. Sleeping sickness in Uganda: a thin line between two fatal diseases. British Medical Journal 331 7527 (2005) 1238-1241
31. Radwanska M., Chamekh M., Vanhamme L., Claes F., Magez S., Magnus E., de Baetselier P., Buscher P., and Pays E. The serum resistance-associated gene as a diagnostic tool for the detection of Trypanosoma brucei rhodesiense. American Journal of Tropical Medicine and Hygiene 67 (2002) 684-690
32. Sloof P., Menke H.H., Caspers M.P.M., and Borst P. Size fractionation of Trypanosoma brucei DNA-localization of the 177-bp repeat satellite DNA and a variant surface glycoprotein gene in a mini-chromosomal DNA fraction. Nucleic Acids Research 11 (1983) 3889-3901
33. Solano P., Jamonneau V., N'Guessan P., N'Dri L., Dje N.N., Miezan T.W., Lejon V., Buscher P., and Garcia A. Comparison of different DNA preparation protocols for PCR diagnosis of Human African Trypanosomosis in Cote d'Ivoire. Acta Tropica 82 (2002) 349-356
34. Solano P., Michel J.F., LeFrancois T., de La Rocque S., Sidibe I., Zoungrana A., and Cuisance D. Polymerase chain reaction as a diagnosis tool for detecting trypanosomes in naturally infected cattle in Burkina Faso. Veterinary Parasitology 86 (1999) 95-103
35. Tilley A., Welburn S.C., Fevre E.M., Feil E.J., and Hide G. Trypanosoma brucei: trypanosome strain typing using PCR analysis of mobile genetic elements (MGE-PCR). Experimental Parasitology 104 (2003) 26-32
36. Vanhamme L., Paturiaux-Hanocq F., Poelvoorde P., Nolan D.P., Lins L., Van Den Abbeele J., Pays A., Tebabi P., Van Xong H., Jacquet A., Moguilevsky N., Dieu M., Kane J.P., De Baetselier P., Brasseur R., and Pays E. Apolipoprotein L-I is the trypanosome lytic factor of human serum. Nature 422 (2003) 83-87
37. Welburn S.C., and Odiit M. Recent developments in human African Trypanosomiasis. Current Opinion in Infectious Disease 15 (2002) 477-484
38. Welburn S.C., Fevre E.M., Odiit M., Maudlin I., and Eisler M.C. Crisis, what crisis? control of rhodesian sleeping sickness. Trends in Parasitology 22 (2006) 123-128
39. Welburn S.C., Picozzi K., Fèvre E.M., Coleman P.G., Odiit M., Carrington M., and Maudlin I. Identification of human infective trypanosomes in animal reservoir of sleeping sickness in Uganda by means of serum resistance associated (SRA) gene. Lancet 358 (2001) 2017-2019
40. Xong H.V., Vanhamme L., Chamekh M., Chimfwembe C.E., Van Den Abbeele J., Pays A., Van Meirvenne N., Hamers R., De Baetselier P., and Pays E. A VSG expression site-associated gene confers resistance to human serum in Trypanosoma rhodesiense. Cell 95 (1998) 839-846
41. Young C.J., and Godfrey D.G. Enzyme polymorphism and the distribution of Trypanosoma congolense isolates. Annals of Tropical Medicine and Parasitology 77 (1983) 467-481
42. Zarlenga D.S., and Higgins J. PCR as a diagnostic and quantitative technique in veterinary parasitology. Veterinary Parasitology 101 (2001) 215-230