Detection of single copy gene sequences from single trypanosomes

Molecular and Biochemical Parasitology

Volumn 84, Issue 2, 1997, Pages 267-270

  MacLeod, Annette ^a   Turner, C Michael R ^a   Tait, Andy ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Dinucleotide repeats; PCR amplification; Trypanosoma brucei

Indexed keywords

CONTROLLED STUDY; DNA SEQUENCE; GENE LOCUS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SHORT SURVEY; TRYPANOSOMA BRUCEI;

TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0031042841     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(96)02810-1     Document Type: Article

References (8)

1. Li, H., Gyllensten, U.B., Gui, X., Saiki, R-K., Erlich, H.A. and Arnheim, N. (1988) Amplification and analysis of DNA sequences in single human sperm and diploid cells. Nature, 355, 414-417.
2. Jeffreys, A.J., Wilson, V., Neumann, R. and Keyte, J. (1988) Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells. Nucleic Acid Res. 16, 10953-10971.
3. Moser, D.R., Cook, G.A., Ochis, D.E., Bailey, C.P., McKane, M.R. and Donelson, J.E. (1989) Detection of Trypanosoma congolense and Trypanosoma brucei subspecies by DNA amplification using the polymerase chain reaction. Parasitology 99, 57-66.
4. Masiga, D.K., Smyth, A.J., Hayes, P., Bromidge, T.J. and Gibson, W.C. (1992) Sensitive detection of trypanosomes in tsetse flies by DNA amplification. Int. J. Parasitol. 22, 909-918.
5. Ranford-Cartwright, L.C., Balfe, P., Carter, R. and Walliker, D. (1991) Genetic hybrids of Plasmodium falciparum identified by amplification of gemonic DNA from single oocysts. Mol. Biochem. Parasitol. 49, 239-244.
6. Swinkels, B.W., Gibson, W.C., Osmga, K.A., Kramer, R., Veneman, G.H., van Boom, J.H. and Borst, P. (1986) Characterisation of the gene for the microbody (glycosomal) triosephosphate isomerase of Trypanosoma brucei. EMBO J. 5, 1291-1298.
7. Turner, C.M.R., Sternberg, J., Buchanan, N., Smith, E., Hide, G. and Tait, A. (1990) Evidence that the mechanism of genetic exchange in T. brucei involves meiosis and syngamy. Parasitology 101, 377-386.
8. Jeffreys, A.J., Neumann, R. and Wilson, V. (1990) Repeat unit sequence variation in minisatellites: a novel source of DNA polymorphism for studying allelic variation and mutation by single molecule analysis. Cell 60, 473-485.