The effect of azathioprine on anastomotic healing: An experimental study in rats

Diseases of the Colon and Rectum

Volumn 50, Issue 12, 2007, Pages 2203-2208

  Stolzenburg, Tilo ^a,b   Ljungmann, Ken ^a   Christensen, Henrik ^a  

^a Århus Universital Hospital THG   (Denmark)

^b AARHUS UNIVERSITY HOSPITAL   (Denmark)

Author keywords

Anastomosis; Animal experimentation; Azathioprine; Breaking strength; Colon; Rats; Surgical

Indexed keywords

6 METHYLTHIOINOSINE; AZATHIOPRINE; PLACEBO; TIOGUANINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; COLON ANASTOMOSIS; CONTROLLED STUDY; DRUG EFFECT; ERYTHROCYTE; EXPERIMENTAL STUDY; FEMALE; METABOLITE; NONHUMAN; RAT;

ANASTOMOSIS, SURGICAL; ANIMALS; AZATHIOPRINE; COLON; DISEASE MODELS, ANIMAL; FEMALE; IMMUNOSUPPRESSIVE AGENTS; RATS; RATS, WISTAR; TREATMENT OUTCOME; WOUND HEALING;

EID: 37249079293     PISSN: 00123706     EISSN: 15300358     Source Type: Journal    
DOI: 10.1007/s10350-007-9010-9     Document Type: Article

References (25)

1. Pearson DC, May GR, Fick GH, Sutherland LR. Azathioprine and 6-mercaptopurine in Crohn's disease. A meta-analysis. Ann Intern Med 1995;123:132-42.
2. Marion JF, Present DH. The modern medical management of acute, severe ulcerative colitis [review]. Eur J Gastroenterol Hepatol 1997;9:831-5.
3. Sandborn WJ. Azathioprine: state of the art in inflammatory bowel disease [review]. Scand J Gastroenterol Suppl 1998;225:92-9.
4. Lennard L. The clinical pharmacology of 6-mercaptopurine [review]. Eur J Clin Pharmacol 1992;43:329-39.
5. Dervieux T, Boulieu R. Simultaneous determination of 6-thioguanine and methyl 6-mercaptopurine nucleotides of azathioprine in red blood cells by HPLC. Clin Chem 1998;44:551-5.
6. George J, Present DH, Pou R. 6-Mercaptopurine for the treatment of ulcerative colitis. Am J Gastroenterol 1996;91:1711-4.
7. Mahadevan U, Loftus EV Jr, Tremaine WJ, et al. Azathioprine or 6-mercaptopurine before colectomy for ulcerative colitis is not associated with increased postoperative complications. Imflamm Bowel Dis 2002;8:311-6.
8. Belliveau P, Truel J, Vasilevsky CA, Stein B, Gordon PH. Ileoanal anastomosis with reservoirs: complications and long-term results. Can J Surg 1999;42:345-52.
9. Meagher AP, Farouk R, Dozois RR, Kelly KA, Pemberton JH. J ileal pouch-anal anastomosis for chronic ulcerative colitis: complications and long-term outcome in 1310 patients. Br J Surg 1998;85:800-3.
10. Hurst RD, Finco C, Rubin M, Michelassi F. Prospective analysis of preoperative morbidity in one hundred consecutive colectomies for ulcerative colitis. Surgery 1995;118:748-55.
11. Anonymous. Report of validation study of assessment of direct immunotoxicity in the rat. International Collaborative Immunotoxicity Study. The ICICIS Group Investigators. Toxicology 1998;125:183-201.
12. Pedersen BK, Beyer JM. A longitudinal study of the influence of azathioprine on natural killer cell activity. Allergy 1986;41:286-9.
13. Institoris L, Siroki O, Desi I, et al. Extension of the protocol of OECD guideline 407 (28-day repeated dose oral toxicity test in the rat) to detect potential immunotoxicity of chemicals. Hum Exp Toxicol 1998;17:206-11.
14. Hendriks T, Mastboom WJ. Healing of experimental intestinal anastomoses. Parameters for repair [review]. Dis Colon Rectum 1990;33:891-901.
15. Jonsson T, Hogstrom H. Neutrophil-dependent decrease in early wound margin strength. Arch Surg 1991;126:1423-6.
16. Christensen H, Chemnitz J, Christensen BC, Oxlund H. Collagen structure organization of healing colonic anastomoses and the effect of growth hormone treatment. Dis Colon Rectum 1995;38:1200-5.
17. Eisinger DR, Sheil AG. A comparison of the effects of cyclosporin A and standard agents on primary wound healing in the rat. Surg Gynecol Obstet 1985;160:135-8.
18. Lima O, Cooper JD, Peters WJ, et al. Effects of methylprednisolone and azathioprine on bronchial healing following lung autotransplantation. Thorac Cardiovasc Surg 1981;82:211-5.
19. Kiyama T, Onda M, Tokunaga A, Efron DT, Barbul A. Effect of matrix metalloproteinase inhibition on colonic anastomotic healing in rats. J Gastrointest Surg 2001;5:303-11.
20. Jiborn H. Healing of left colonic anastomoses. An experimental study in the rat [thesis]. Malmö: University of Lund, 1978.
21. Oxlund H, Christensen H, Seyer-Hansen M, Andreassen TT. Collagen deposition and mechanical strength of colon anastomoses and skin incisional wounds of rats. J Surg Res 1996;66:25-30.
22. Fielding LP, Steward-Brown S, Blesovsky L, Kearney G. Anastomotic integrity after operations for large bowel cancer: a multicenter study. Br Med J 1980;281:411-4.
23. Sandborn WJ, Tremaine WJ, Wolf DC, et al. Lack of effect of intravenous administration on time to respond to azathioprine for steroid-treated Crohn's disease. North American Azathioprine Study Group. Gastroenterology 1999;117:527-35.
24. Dubinsky MC, Lamothe S, Yang HY, et al. Pharmacogenomics and metabolite measurement for 6-mercaptopurine therapy in inflammatory bowel disease. Gastroenterology 2000;118:705-13.
25. Belaiche J, Desager JP, Horsmans Y, Louis E. Therapeutic drug monitoring of azathioprine and 6-mercaptopurine metabolites in Crohn's disease. Scand J Gastroenterol 2001;36:71-6.