Extraction of dengue 2 plasmid DNA vaccine (pD2) from cell lysates by aqueous two-phase systems

Biotechnology

Volumn 6, Issue 4, 2007, Pages 520-526

  Moreira, Keila Aparecida ^a,b,f   Chaves, Adilson Castro ^c   Marques, Ernesto Torres ^d   Prazeres, Duarte Miguel Ferreira ^e   De Azevedo, Walter Mendes ^a   Porto, Ana Lúcia Figueiredo ^a,b   De Filho, José Luiz Lima ^a  

^a FEDERAL UNIVERSITY OF PERNAMBUCO   (Brazil)

^b UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO   (Brazil)

^c Instituto de Ciências Biológicas ICB e Ciências Exatas e Naturais   (Brazil)

^d JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e INSTITUTO SUPERIOR TÉCNICO   (Portugal)

^f NONE   (Brazil)

Author keywords

Aqueous two phase systems; Liquid liquid extraction; Plasmid DNA

Indexed keywords

CELLS; DISEASE CONTROL; ENZYME ACTIVITY; MOLECULAR WEIGHT; PROTEINS;

AQUEOUS TWO PHASE SYSTEMS; CELL LYSATES; LIQUID LIQUID EXTRACTION; PLASMID DNA;

DNA;

ESCHERICHIA COLI;

EID: 37249075900     PISSN: 1682296X     EISSN: 16822978     Source Type: Journal    
DOI: 10.3923/biotech.2007.520.526     Document Type: Review

References (24)

1. Albertsson, P.A., 1986. Partition of Cell Particles and Macromolecules. Jonh Wiley, New York.
2. Albertsson, P.A., 1962. Partition of double-stranded and single-stranded deoxyribonucleic acid. Arch. Biochem. Biophys., 1: 264-270.
3. Diogo, M.M., J.A. Queiroz, G.A. Monteiro, G.N.M. Ferreira, S.A.M. Martins and D.M.F. Prazeres, 2000. Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography. Biotechnol. Bioeng., 68: 576-583.
4. Hatti-Kaul, R., 1999. Methods in Biotechnology, V. 11, Humana Press, New Jersey.
5. Huddleston, J., K.W. Ottomar, D. Ngonyani, J.A. Flanagan and A. Lyddiat, 1990. In: Separations for Biotechnology. Pyle, D.L. (Ed.), Elsevier Applied Science, London, pp: 181-190.
6. Huddleston, J., A. Veide, K. Kohler, J. Flanagan, S.O. Enfors and A. Lyddiat, 1991. The molecular basis of partitioning in aqueous two-phase systems. Trends. Biotechnol., 9: 381-388.
7. Johansson, H.O., G. Karlstrom, F. Tjerneld and C.A. Haynes, 1998. Driving forces for phase separation and partitioning in aqueous two-phase systems. J. Chromatogr., B 718: 3-17.
8. Kepka, C., J. Rhodin, R. Lemmens, F. Tjerneld, P.E. Gustavsson, 2004. Extraction of plasmid DNA from Escherichia coli cell lysate in a thermoseparation aqueous two-phase system. J. Chromatogr., A 1024: 95-104.
9. Kimura, K., 2000. Simultaneous accumulation of low-molecular-mass RNA at the interface along with accumulation of high-molecular-mass RNA on aqueous two-phase system partitioning. J. Chromatogr., B 743: 421-429.
10. Lemmens, R., U. Olsson, T. Nyhammar and J. Stadier, 2003. Supercoiled plasmid DNA: Selective purification by thiophilic/aromatic adsorption. J. Chromatogr., B 784: 291-300.
11. Lu, Y., K. Raviprakash, I.C. Leão, P.R. Chikhlikar, D. Ewing, A. Anwar, C. Chougnet, G. Murphy, C.G. Hayes, T.J. August and E.T. Marques, 2003. Dengue 2 PreM-E/LAmp chimera targeted to the MHC class II compartment elicits long-lasting neutralizing antibodies. Vaccine, 21: 2178-2189.
12. Moreira, K.A., M.M. Diogo, D.M.F. Prazeres, J.L. Lima Filho and A.L.F. Porto, 2005. Purification of plasmid (pVaxLacZ) by hydrophobic Interaction Chromatography. Braz. Arch. Biol. Tech., 48: 113-117.
13. Mountain, A., 2000. Gene therapy: The first decade Review. Tibtech, 18: 119-128.
14. Prazeres, D.M.F., G.A. Monteiro, G.N.M. Ferreira, M.M. Diogo, S.C. Ribeiro, J.M.S. Cabral, 2001. In: Biotechnology Annual Review. El-Gewely, M.R. (Ed.), Elsevier. Netherlands, pp: 1.
15. Ribeiro, S.C., G.A. Monteiro, G. Martinho, J.M.S. Cabral and D.M.F. Prazeres, 2000. Quatitation of plasmid DNA in aqueous two-phase systems by fluorescence analysis. Biotechnol. Lett., 22: 1101-1104.
16. Ribeiro, S.C., G. A. Monteiro, J.M.S. Cabral and D.M.F. Prazeres, 2002. Isolation of plasmid DNA from cell lysates by aqueous two-phase systems. Biotechnol. Bioeng., 78: 376-384.
17. Rito-Palomares, M. and A.P.J. Middelberg, 2002. Aqueous two-phase systems for the recovery of a recombinant viral coat protein from Escherichia coli. J. Chem. Tech. Biotech., 77: 1025-1029.
18. Rito-Palomares, M., 2004. Practical application of aqueous two-phase partition to process development for the recovery of biological products. J. Chromatogr., B 807: 3-11.
19. Sambrook, J., E.F. Fritsch and T. Maniatis, 1989. Molecular Cloning. A Laboratory Manual. Cold Spring Harbour Laboratoy Press, New York.
20. Shang, Q.K., W. Li, Q. Li and D.Q. Li, 2004. Partitioning behavior of amino acids in aqueous two-phase systems containing polyethylene glycol and phosphate buffer. Fluid Phase Equil., 219: 195-203.
21. Shibusawa, Y., Y. Ino, T. Kinebuchi, M. Shimizu, H. Shi ndo and Y. Ito, 2003. Purification of single-stranded DNA binding protein from an Escherichia coli lysate using counter-current chromatography, partition and precipitation. J. Chromatogr., B 793: 275-279.
22. Trindade, I.P., M.M. Diogo, D.M.F. Prazeres and J.C. Marcos, 2005. Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography. J. Chromatogr., A 1082: 176-184.
23. Vasilevskaya, V. V., A. R Khokhlov, Y. Matsuzawa and K. Yoshikawa, 1995. Collapse of single DNA molecule in poly(ethylene glycol) solutions. J. Chem. Phys., 102: 6595-6602.
24. Wang, Z., G. Le, Y. Shi and G. Wegrzyn, 2001. Medium design for plasmid DNA production based on stoichiometric model. Proc. Biochem., 36: 1085-1093.