Topology of the selectivity filter of a TRPV channel: Rapid accessibility of contiguous residues from the external medium

American Journal of Physiology - Cell Physiology

Volumn 293, Issue 6, 2007, Pages

  Dodier, Yolaine ^a   Dionne, François ^a   Raybaud, Alexandra ^a   Sauvé, Rémy ^a   Parent, Lucie ^a  

^a UNIVERSITÉ DE MONTRÉAL   (Canada)

Author keywords

Calcium; Cysteine; Electrophysiology; Ion channel; Kidney; Methane thiosulfonate reagents; Site directed mutagenesis; Three dimensional homology modeling; Transient receptor potential; Transport

Indexed keywords

2 (AMINOETHYL)METHANE THIOSULFONATE BROMIDE; 2 (TRIMETHYL AMMONIUM)ETHYL METHANE THIOSULFONATE BROMIDE; 2 (TRIMETHYL AMMONIUM)METHYL METHANE THIOSULFONATE BROMIDE; BROMINE DERIVATIVE; CARBOXYLIC ACID; CYSTEINE; THIOSULFINIC ACID DERIVATIVE; UNCLASSIFIED DRUG; VANILLOID RECEPTOR;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CHEMICAL STRUCTURE; ELECTROPHYSIOLOGY; FEMALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANIMALS; ASPARTIC ACID; BINDING SITES; CALCIUM; CELL MEMBRANE; EXTRACELLULAR SPACE; FEMALE; INDICATORS AND REAGENTS; ISOLEUCINE; MESYLATES; PROTEIN CONFORMATION; RABBITS; TRPV CATION CHANNELS; XENOPUS LAEVIS;

EID: 37149014268     PISSN: 03636143     EISSN: 15221563     Source Type: Journal    
DOI: 10.1152/ajpcell.00406.2007     Document Type: Article

References (26)

1. Aiyar J, Rizzi JP, Gutman GA, Chandy KG. The signature sequence of voltage-gated potassium channels projects into the external vestibule. J Biol Chem 271: 31013-31016, 1996.
2. Berrou L, Klein H, Bernatchez G, Parent L. A specific tryptophan in the I-II linker is a key determinant of β-subunit binding and modulation in CaV2.3 calcium channels. Biophys J 83: 1429-1442, 2002.
3. Cui Y, Wang W, Fan Z. Cytoplasmic vestibule of the weak inward rectifier Kir6.2 potassium channel. J Biol Chem 277: 10523-10530, 2002.
4. Dodier Y, Banderali U, Klein H, Topalak O, Dafi O, Simoes M, Bernatchez G, Sauve R, Parent L. Outer pore topology of the ECaC-TRPV5 channel by cysteine scan mutagenesis. J Biol Chem 279: 6853-6862, 2004.
5. Fabiato A, Fabiato F. Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells. J Physiol 75: 463-505, 1979.
6. 3-responsive epithelia. J Biol Chem 274: 8375-8378, 1999.
7. 2+ channel family: comparison of mammalian ECaC1 and 2. J Physiol 537: 747-761, 2001.
8. Holmgren M, Liu Y, Xu Y, Yellen G. On the use of thiol-modifying agents to determine channel topology. Neuropharmacology 35: 797-804, 1996.
9. Jean K, Bernatchez G, Klein H, Garneau L, Sauve R, Parent L. Role of aspartate residues in Ca affinity and permeation of the distal ECaC1. Am J Physiol Cell Physiol 282: C665-C672, 2002.
10. Lee J, Cha SK, Sun TJ, Huang CL. PIP2 activates TRPV5 and releases its inhibition by intracellular Mg. J Gen Physiol 126: 439-451, 2005.
11. Liu YS, Sompornpisut P, Perozo E. Structure of the KcsA channel intracellular gate in the open state. Nat Struct Biol 8: 883-887, 2001.
12. Long SB, Campbell EB, MacKinnon R. Crystal structure of a mammalian voltage-dependent Shaker family K channel. Science 309: 897-903, 2005.
13. Lu T, Zhu YG, Yang J. Cytoplasmic amino and carboxyl domains form a wide intracellular vestibule in an inwardly rectifying potassium channel. Proc Natl Acad Sci USA 96: 9926-9931, 1999.
14. 2+ channel. J Biol Chem 276: 1020-1025, 2001.
15. 2+-dependent inactivation. Pflügers Arch 445: 584-588, 2003.
16. 2+ channel. Biophys J 69: 1801-1813, 1995.
17. 1E calcium channel. J Membr Biol 160: 127-140, 1997.
18. Ramsey IS, Delling M, Clapham DE. An introduction to TRP channels. Annu Rev Physiol 68: 619-647, 2006.
19. 2+ channels. J Biol Chem 281: 39424 -39436, 2006.
20. Sali A, Blundell TL. Comparative protein modelling by satisfaction of spatial restraints. J Mol Biol 234: 779-815, 1993.
21. +-conducting channel. Nature 440: 570-574, 2006.
22. Simoes M, Garneau L, Klein H, Banderali U, Hobeila F, Roux B, Parent L, Sauve R. Cysteine mutagenesis and computer modeling of the S6 region of an intermediate conductance IKCa channel. J Gen Physiol 120: 99-116, 2002.
23. 2+-selective TRP channel. J Biol Chem 279: 15223-15230, 2004.
24. 2+-dependent gating and strong inward rectification of the cation channel TRPV6. J Gen Physiol 121: 245-260, 2003.
25. Yeh BI, Kim YK, Jabbar W, Huang CL. Conformational changes of pore helix coupled to gating of TRPV5 by protons. EMBO J 24: 3224-3234, 2005.
26. + channel-Fab complex at 2.0 A resolution. Nature 414: 43-48, 2001.