Catalytically active soluble ecto-5'-nucleotidase purified after heterologous expression as a tool for drug screening

Drug Development Research

Volumn 45, Issue 3-4, 1998, Pages 269-276

  Servos, Jörg ^a  

^a UNIVERSITY HOSPITAL FRANKFURT   (Germany)

Author keywords

5' nucleotidase; AMP; ATP; Ecto nucleotidase

Indexed keywords

ECTO 5' NUCLEOTIDASE;

CONFERENCE PAPER; DRUG SCREENING; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; NONHUMAN; PROTEIN EXPRESSION; SCHISTOSOMA JAPONICUM;

EID: 0032468792     PISSN: 02724391     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2299(199811/12)45:3/4<269::AID-DDR25>3.0.CO;2-B     Document Type: Conference Paper

References (4)

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3. Bailyes EM, Newby AC, Siddle K, Luzio JE 1982. Solubilization and purification of rat liver 5-nucleotidase by use of a zwitterionic detergent and a monoclonal-antibody immunoadsorbent. Biochem J 203:245-251.
4. Chen BC, Lee CM, Lin WW. 1996. Inhibition of ecto-ATPase by PPADS, suramin and reactive blue in endothelial cells, C-6 glioma cells and RAW 264.7 macrophages. Br J Pharmacol 119:1628-1634.