Biomaterial osseointegration enhancement with biophysical stimulation

Journal of Musculoskeletal Neuronal Interactions

Volumn 7, Issue 3, 2007, Pages 253-265

  Dimitriou, R ^a   Babis, G C ^a  

^a UNIVERSITY OF ATHENS MEDICAL SCHOOL   (Greece)

Author keywords

Biophysical stimulation; LIPUS; Osseointegration; PEMFs

Indexed keywords

BONE MORPHOGENETIC PROTEIN; BONE MORPHOGENETIC PROTEIN 2; FIBRONECTIN; IBANDRONIC ACID; OSTEOGENIC PROTEIN 1; PARATHYROID HORMONE[1-34]; PLATELET DERIVED GROWTH FACTOR; SOMATOMEDIN; SOMATOMEDIN C; TRANSFORMING GROWTH FACTOR BETA1; TRANSFORMING GROWTH FACTOR BETA2; VITRONECTIN;

ADJUVANT THERAPY; BIOCOMPATIBILITY; BIOLOGICAL ACTIVITY; BONE GRAFT; BONE REGENERATION; CONFERENCE PAPER; ELECTROMAGNETIC FIELD; FRACTURE HEALING; IMPLANT; LOW ENERGY RADIATION; ULTRASOUND THERAPY;

ABSORBABLE IMPLANTS; ANIMALS; BONE AND BONES; ELECTRIC STIMULATION THERAPY; ELECTROMAGNETIC FIELDS; FRACTURE HEALING; FRACTURES, BONE; HUMANS; INTERNAL FIXATORS; OSSEOINTEGRATION; ULTRASONIC THERAPY;

EID: 36949033650     PISSN: 11087161     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (164)

1. LeGeros RZ, Craig RG. Strategies to affect bone remodeling: osteointegration. J Bone Miner Res 1993; 8(Suppl.2):S583-596.
2. Morra M, Cassinelli C, Cascardo G, Cahalan P, Cahalan L, Fini M, Giardino R. Surface engineering of titanium by collagen immobilization. Surface characterization and in vitro and in vivo studies. Biomaterials 2003; 24:4639-4654.
3. Brånemark P-I. Vital microscopy of bone marrow in rabbit. Scand J Clin Lab Invest 1959; 11(Suppl.38):1-82.
4. Brånemark PI, Hansson BO, Adell R, Breine U, Lindstrom J, Hallen O, Ohman A. Osseointegrated implants in the treatment of the edentulous jaw. Experience from a 10-year period. Scand J Plast Reconstr Surg Suppl 1977; 16:1-132.
5. Brånemark P-I. Osseointegration and its experimental studies. J Prosthet Dent 1983; 50:399-410.
6. Rigo ECS, Boschi AO, Yoshimoto M, Allegrini S Jr, Konig B Jr, Carbonari MJ. Evaluation in vitro and in vivo of biomimetic hydroxyapatite coated on titanium dental implants. Materials Science and Engineering C 2004; 24:647-651.
7. Soballe K. Hydroxyapatite ceramic coating for bone implant fixation. Mechanical and histological studies in dogs. Acta Orthop Scand Suppl 1993; 255:1-58.
8. Linder L, Obrant K, Boivin G. Osseointegration of metallic implants. II. Transmission electron microscopy in the rabbit. Acta Orthop Scand 1989; 60:135-139.
9. Park JY, Davies JE. Red blood cell and platelet interactions with titanium implant surfaces. Clin Oral Implants Res 2000; 11:530-539.
10. Boyan BD, Bonewald LF, Paschalis EP, Lohmann CH, Rosser J, Cochran DL, Dean DD, Schwartz Z, Boskey AL. Osteoblast-mediated mineral deposition in culture is dependent on surface microtopography. Calcif Tissue Int 2002; 71:519-529.
11. Davies JE. Mechanisms of endosseous integration. Int J Prosthodont 1998; 11:391-401.
12. Nygren H, Eriksson C, Lausmaa J. Adhesion and activation of platelets and polymorphonuclear granulocyte cells at TiO2 surfaces. Lab Clin Med 1997; 129:35-46.
13. Sundgren JE, Bodo P, Lundstrom I, Berggren A, Hellem S. Auger electron spectroscopic studies of stainless-steel implants. J Biomed Mater Res 1985; 19:663-671.
14. Meyer U, Joos U, Mythili J, Stamm T, Hohoff A, Fillies T, Stratmann U, Wiesmann HP. Ultrastructural characterization of the implant/bone interface of immediately loaded dental implants. Biomaterials 2004; 25:1959-1967.
15. Albrektsson T, Hansson HA. An ultrastructural characterization of the interface between bone and sputtered titanium or stainless steel surfaces. Biomaterials 1986; 7:201-205.
16. Puleo DA, Nanci A. Understanding and controlling the bone-implant interface. Biomaterials 1999; 20:2311-2321.
17. Chappard D, Aguado E, Hure G, Grizon F, Basle MF. The early remodeling phases around titanium implants: a histomorphometric assessment of bone quality in a 3- and 6-month study in sheep. Int J Oral Maxillofac Implants 1999; 14:189-196.
18. Marco F, Milena F, Gianluca G, Vittoria O. Peri-implant osteogenesis in health and osteoporosis. Micron 2005; 36:630-644.
19. Hofmann AA, Bloebaum RD, Bachus KN. Progression of human bone ingrowth into porous-coated implants. Rate of bone ingrowth in humans. Acta Orthop Scand 1997; 68:161-166.
20. Kim YH, Kim VE. Uncemented porous-coated anatomic total hip replacement. Results at six years in a consecutive series. J Bone Joint Surg Br 1993; 75:6-13.
21. Anselme K. Osteoblast adhesion on biomaterials. Biomaterials 2000; 21:667-681.
22. Breme J, Steinhauser E, Paulus G. Commercially pure titanium Steinhauser plate-screw system for maxillofacial surgery. Biomaterials 1988; 9:310-313.
23. Browne M, Gregson PJ. Effect of mechanical surface pretreatment on metal ion release. Biomaterials 2000; 21:385-392.
24. Brune D, Evje D, Melsom S. Corrosion of gold alloys and titanium in artificial saliva. Scand J Dent Res 1982; 90:168-171.
25. Rae T. A study on the effects of particulate metals of orthopaedic interest on murine macrophages in vitro. J Bone Joint Surg Br 1975; 57:444-450.
26. Rae T. The toxicity of metals used in orthopaedic prostheses. An experimental study using cultured human synovial fibroblasts. J Bone Joint Surg Br 1981; 63-B:435-440.
27. Alberius P. Bone reactions to tantalum markers. A scanning electron microscopic study. Acta Anat (Basel). 1983; 115:310-318.
28. Johansson CB, Albrektsson T. A removal torque and histomorphometric study of commercially pure niobium and titanium implants in rabbit bone. Clin Oral Implants Res 1991; 2:24-29.
29. Kujala S, Ryhanen J, Danilov A, Tuukkanen J. Effect of porosity on the osteointegration and bone ingrowth of a weight-bearing nickel-titanium bone graft substitute. Biomaterials 2003; 24:4691-4697.
30. Thomsen P, Larsson C, Ericson LE, Sennerby L, Lausmaa J, Kasemo B. Structure of the interface between rabbit cortical bone and implants of gold, zirconium and titanium. J Mater Sci Mater Med 1997; 8:653-665.
31. Mohammadi S, Esposito M, Cucu M, Ericson LE, Thomsen P. Tissue response to hafnium. J Mater Sci Mater Med 2001; 12:603-611.
32. Kurzweg H, Heimann RB, Troczynski T, Wayman ML. Development of plasma-sprayed bioceramic coatings with bond coats based on titania and zirconia. Biomaterials 1998; 19:1507-1511.
33. Soballe K, Overgaard S, Hansen ES, Brokstedt-Rasmussen H, Lind M, Bunger C. A review of ceramic coatings for implant fixation. J Long Term Eff Med Implants 1999; 9:131-151.
34. Cochran DL, Nummikoski PV, Higginbottom FL, Hermann JS, Makins SR, Buser D. Evaluation of an endosseous titanium implant with a sandblasted and acid-etched surface in the canine mandible: radiographic results. Clin Oral Implants Res 1996; 7:240-252.
35. Larsson C, Thomsen P, Aronsson BO, Rodahl M, Lausmaa J, Kasemo B, Ericson LE. Bone response to surface-modified titanium implants: studies on the early tissue response to machined and electropolished implants with different oxide thicknesses. Biomaterials 1996; 17:605-616.
36. Borsari V, Giavaresi G, Fini M, Torricelli P, Tschon M, Chiesa R, Chiusoli L, Salito A, Volpert A, Giardino R. Comparative in vitro study on a ultra-high roughness and dense titanium coating. Biomaterials 2005; 26:4948-4955.
37. Fini M, Giardino R. In vitro and in vivo tests for the biological evaluation of candidate orthopedic materials: benefits and limits. Journal of Applied Biomaterials & Biomechanics 2003; 1:155-163.
38. Albrektsson T, Wennerberg A. Oral implant surfaces: Part 2-review focusing on clinical knowledge of different surfaces. Int J Prosthodont 2004; 17:544-564.
39. Bobyn JD, Pilliar RM, Cameron HU, Weatherly GC. The optimum pore size for the fixation of porous-surfaced metal implants by the ingrowth of bone. Clin Orthop Relat Res 1980; 150:263-270.
40. Galois L, Mainard D. Bone ingrowth into two porous ceramics with different pore sizes: an experimental study. Acta Orthop Belg 2004; 70:598-603.
41. Linder L, Obrant K, Boivin G. Osseointegration of metallic implants. II. Transmission electron microscopy in the rabbit. Acta Orthop Scand 1989; 60:135-139.
42. Spadaro JA, Albanese SA, Chase SE. Electromagnetic effects on bone formation at implants in the medullary canal in rabbits. J Orthop Res 1990; 8:685-693.
43. Wittenberg RH, Shea M, Swartz DE, Lee KS, White AA III, Hayes WC. Importance of bone mineral density in instrumented spine fusions. Spine 1991; 16:647-652.
44. de Vicente JC, Recio O, Martin-Villa L, Junquera LM, Lopez-Arranz JS. Histomorphometric evaluation of guided bone regeneration around implants with SLA surface: an experimental study in beagle dogs. Int J Oral Maxillofac Surg 2006; 35:1047-1053.
45. Giori NJ, Ryd L, Carter DR. Mechanical influences on tissue differentiation at bone-cement interfaces. J Arthroplasty 1995; 10:514-522.
46. Sennerby L, Thomsen P, Ericson LE. A morphometric and biomechanic comparison of titanium implants inserted in rabbit cortical and cancellous bone. Int J Oral Maxillofac Implants 1992; 7:62-71.
47. Khan SN, Cammisa FP Jr, Sandhu HS, Diwan AD, Girardi FP, Lane JM. The biology of bone grafting. J Am Acad Orthop Surg 2005; 13:77-86.
48. Arrington ED, Smith WJ, Chambers HG, Bucknell AL, Davino NA. Complications of iliac crest bone graft harvesting. Clin Orthop 1996; 329:300-309.
49. Younger EM, Chapman MW. Morbidity at bone graft donor sites. J Orthop Trauma 1989; 3:192-195.
50. Lynch SE, Buser D, Hernandez RA, Weber HP, Stich H, Fox CH, Williams RC. Effects of the platelet-derived growth factor/insulin-like growth factor-I combination on bone regeneration around titanium dental implants. Results of a pilot study in beagle dogs. J Periodontol 1991; 62:710-716.
51. Lamberg A, Schmidmaier G, Soballe K, Elmengaard B. Locally delivered TGF-beta1 and IGF-1 enhance the fixation of titanium implants: a study in dogs. Acta Orthop 2006; 77:799-805.
52. Mannai C. Early implant loading in severely resorbed maxilla using xenograft, autograft, and platelet-rich plasma in 97 patients. J Oral Maxillofac Surg 2006; 64:1420-1426.
53. Sumner DR, Turner TM, Urban RM, Virdi AS, Inoue N. Additive enhancement of implant fixation following combined treatment with rhTGF-beta2 and rhBMP-2 in a canine model. J Bone Joint Surg Am 2006; 88:806-817.
54. Cole BJ, Bostrom MP, Pritchard TL, Sumner DR, Tomin E, Lane JM, Weiland AJ. Use of bone morphogenetic protein 2 on ectopic porous coated implants in the rat. Clin Orthop Relat Res 1997; 219-228.
55. Koempel JA, Patt BS, O'Grady K, Wozney J, Toriumi DM. The effect of recombinant human bone morphogenetic protein-2 on the integration of porous hydroxyapatite implants with bone. J Biomed Mater Res 1998; 41:359-363.
56. Jennissen HP. Accelerated and improved osteointegration of implants biocoated with bone morphogenetic protein 2 (BMP-2). Ann N Y Acad Sci 2002; 961:139-142.
57. Becker J, Kirsch A, Schwarz F, Chatzinikolaidou M, Rothamel D, Lekovic V, Laub M, Jennissen HP. Bone apposition to titanium implants biocoated with recombinant human bone morphogenetic protein-2 (rhBMP-2). A pilot study in dogs. Clin Oral Investig 2006; 10:217-224.
58. Lind M, Overgaard S, Song Y, Goodman SB, Bunger C, Soballe K. Osteogenic protein 1 device stimulates bone healing to hydroxyapaptite-coated and titanium implants. J Arthroplasty 2000; 15:339-346.
59. Zhang R, An Y, Toth CA, Draughn RA, Dimaano NM, Hawkins MV. Osteogenic protein-1 enhances osseointegration of titanium implants coated with peri-apatite in rabbit femoral defect. J Biomed Mater Res B Appl Biomater 2004; 71:408-413.
60. Sumner DR, Turner TM, Purchio AF, Gombotz WR, Urban RM, Galante JO. Enhancement of bone ingrowth by transforming growth factor-beta. J Bone Joint Surg Am 1995; 77:1135-1147.
61. De Ranieri A, Virdi AS, Kuroda S, Shott S, Leven RM, Hallab NJ, Sumner DR. Local application of rhTGF-beta2 enhances peri-implant bone volume and bone-implant contact in a rat model. Bone 2005; 37:55-62.
62. Salata LA, Burgos PM, Rasmusson L, Novaes AB, Papalexiou V, Dahlin C, Sennerby L. Osseointegration of oxidized and turned implants in circumferential bone defects with and without adjunctive therapies: an experimental study on BMP-2 and autogenous bone graft in the dog mandible. Int J Oral Maxillofac Surg 2007; 36:62-71.
63. Geissler U, Hempel U, Wolf C, Scharnweber D, Worch H, Wenzel K. Collagen type I coating of Ti6A14V promotes adhesion of osteoblasts. J Biomed Mater Res 2000; 51:752-760.
64. Rammelt S, Schulze E, Bernhardt R, Hanisch U, Scharnweber D, Worch H, Zwipp H, Biewener A. Coating of titanium implants with type-I collagen. J Orthop Res 2004; 22:1025-1034.
65. Ku Y, Chung CP, Jang JH. The effect of the surface modification of titanium using a recombinant fragment of fibronectin and vitronectin on cell behaviour. Biomaterials 2005; 26:5153-5157.
66. Frosch KH, Sondergeld I, Dresing K, Rudy T, Lohmann CH, Rabba J, Schild D, Breme J, Stuermer KM. Autologous osteoblasts enhance osseointegration of porous titanium implants. J Orthop Res 2003; 21:213-223.
67. Eberhardt C, Habermann B, Tiemann S, Bauss F, Kurth AA. Improvement of osseointegration of cementless metal implant under Ibandronate is dose-dependent. Bone 2006; 38:S42-S64 (abstract).
68. Gabet Y, Muller R, Levy J, Dimarchi R, Chorev M, Bab I, Kohavi D. Parathyroid hormone 1-34 enhances titanium implant anchorage in low-density trabecular bone: a correlative micro-computed tomographic and biomechanical analysis. Bone 2006; 39:276-282.
69. Pilliar RM, Lee JM, Maniatopoulos C. Observations on the effect of movement on bone ingrowth into porous-surfaced implants. Clin Orthop Relat Res 1986; 108-113.
70. Otsuki B, Takemoto M, Fujibayashi S, Neo M, Kokubo T, Nakamura T. Pore throat size and connectivity determine bone and tissue ingrowth into porous implants: three-dimensional micro-CT based structural analyses of porous bioactive titanium implants. Biomaterials 2006; 27:5892-5900.
71. Berglundh T, Abrahamsson I, Lang NP, Lindhe J. De novo alveolar bone formation adjacent to endosseous implants. Clin Oral Implants Res 2003; 14:251-262.
72. Sandborn PM, Cook SD, Spires WP, Kester MA. Tissue response to porous-coated implants lacking initial bone apposition. J Arthroplasty 1988; 3:337-346.
73. Kudo M, Matsui Y, Ohno K, Michi K. A histomorphometric study of the tissue reaction around hydroxyapatite implants irradiated after placement. J Oral Maxillofac Surg 2001; 59:293-300.
74. Sumner DR, Turner TM, Pierson RH, Kienapfel H, Urban RM, Liebner EJ, Galante JO. Effects of radiation on fixation of non-cemented porous-coated implants in a canine model. J Bone Joint Surg Am 1990; 72:1527-1533.
75. Brasseur M, Brogniez V, Gregoire V, Reychler H, Lengele B, D'Hoore W, Nyssen-Behets C. Effects of irradiation on bone remodelling around mandibular implants: an experimental study in dogs. Int J Oral Maxillofac Surg 2006; 35:850-855.
76. Bolind P, Johansson CB, Johansson P, Granstrom G, Albrektsson T. Retrieved implants from irradiated sites in humans: a histologic/ histomorphometric investigation of oral and craniofacial implants. Clin Implant Dent Relat Res 2006; 8:142-150.
77. Sakakura CE, Marcantonio E Jr, Wenzel A, Scaf G. Influence of cyclosporin A on quality of bone around integrated dental implants: a radiographic study in rabbits. Clin Oral Implants Res 2007; 8:34-39.
78. Eder A, Watzek G. Treatment of a patient with severe osteoporosis and chronic polyarthritis with fixed implant-supported prosthesis: a case report. Int J Oral Maxillofac Implants 1999; 14:587-590.
79. McDonald AR, Pogrel MA, Sharma A. Effects of chemotherapy on osseointegration of implants: a case report. J Oral Implantol 1998; 24:11-13.
80. Callahan BC, Lisecki EJ, Banks RE, Dalton JE, Cook SD, Wolff JD. The effect of warfarin on the attachment of bone to hydroxyapatite-coated and uncoated porous implants. J Bone Joint Surg Am 1995; 77:225-230.
81. Cook SD, Barrack RL, Dalton JE, Thomas KA, Brown TD. Effects of indomethacin on biologic fixation of porous-coated titanium implants. J Arthroplasty 1995; 10:351-358.
82. Rosenqvist R, Bylander B, Knutson K, Rydholm U, Rooser B, Egund N, Lidgren L. Loosening of the porous coating of bicompartmental prostheses in patients with rheumatoid arthritis. J Bone Joint Surg Am 1986; 68:538-542.
83. Zhang H, Lewis CG, Aronow MS, Gronowicz GA. The effects of patient age on human osteoblasts' response to Ti-6Al-4V implants in vitro. J Orthop Res 2004; 22:30-38.
84. Mombelli A, Cionca N. Systemic diseases affecting osseointegration therapy. Clin Oral Implants Res 2006; 17(Suppl.2):97-103.
85. Wong MM, Rao LG, Ly H, Hamilton L, Ish-Shalom S, Sturtridge W, Tong J, McBroom R, Josse RG, Murray TM. In vitro study of osteoblastic cells from patients with idiopathic osteoporosis and comparison with cells from non-osteoporotic controls. Osteoporos Int 1994; 4:21-31.
86. D'Ippolito G, Schiller PC, Ricordi C, Roos BA, Howard GA. Age-related osteogenic potential of mesenchymal stromal stem cells from human vertebral bone marrow. J Bone Miner Res 1999; 14:1115-1122.
87. Kubo T, Shiga T, Hashimoto J, Yoshioka M, Honjo H, Urabe M, Kitajima I, Semba I, Hirasawa Y. Osteoporosis influences the late period of fracture healing in a rat model prepared by ovariectomy and low calcium diet. J Steroid Biochem Mol Biol 1999; 68:197-202.
88. Namkung-Matthai H, Appleyard R, Jansen J, Hao Lin J, Maastricht S, Swain M, Mason RS, Murrell GA, Diwan AD, Diamond T. Osteoporosis influences the early period of fracture healing in a rat osteoporotic model. Bone 2001; 28:80-86.
89. Blomqvist JE, Alberius P, Isaksson S, Linde A, Hansson BG. Factors in implant integration failure after bone grafting: an osteometric and endocrinologic matched analysis. Int J Oral Maxillofac Surg 1996; 25:63-68.
90. Augat P, Simon U, Liedert A, Claes L. Mechanics and mechano-biology of fracture healing in normal and osteoporotic bone. Osteoporos Int 2005; 16(Suppl.2):S36-43.
91. Fini M, Giavaresi G, Torricelli P, Borsari V, Giardino R, Nicolini A, Carpi A. Osteoporosis and biomaterial osteointegration. Biomed Pharmacother 2004; 58:487-493.
92. Fini M, Nicoli Aldini N, Gandolfi MG, Mattioli Belmonte M, Giavaresi G, Zucchini C, De Benedittis A, Amati S, Ravaglioli A, Krayewski A, Rocca M, Guzzardella GA, Biagini G, Giardino R. Biomaterials for orthopedic surgery in osteoporotic bone: a comparative study in osteopenic rats. Int J Artif Organs 1997; 20:291-297.
93. Fini M, Giavaresi G, Torricelli P, Krajewski A, Ravaglioli A, Belmonte MM, Biagini G, Giardino R. Biocompatibility and osseointegration in osteoporotic bone. J Bone Joint Surg Br 2001; 83:139-143.
94. Hayashi K, Uenoyama K, Mashima T, Sugioka Y. Remodelling of bone around hydroxyapatite and titanium in experimental osteoporosis. Biomaterials 1994; 15:11-16.
95. Rocca M, Fini M, Giavaresi G, Nicoli Aldini N, Giardin R. Tibial implants: biomechanical and histomorphometric studies of hydroxyapatite-coated and uncoated stainless steel and titanium screws in long-term ovariectomized sheep. Int J Artif Organs 2001; 24:649-654.
96. Bassett CA, Pawluk RJ, Pilla AA. Augmentation of bone repair by inductively coupled electromagnetic fields. Science 1974; 184:575-577.
97. Greenough CG. The effects of pulsed electromagnetic fields on blood vessel growth in the rabbit ear chamber. J Orthop Res 1992; 10:256-262.
98. Roland D, Ferder M, Kothuru R, Faierman T, Strauch B. Effects of pulsed magnetic energy on a microsurgically transferred vessel. Plast Reconstr Surg 2000; 105: 1371-1374.
99. Smith TL, Wong-Gibbons D, Maultsby J. Microcirculatory effects of pulsed electromagnetic fields. J Orthop Res 2004; 22:80-84.
100. Fredericks DC, Piehl DJ, Baker JT, Abbott J, Nepola JV. Effects of pulsed electromagnetic field stimulation on distraction osteogenesis in the rabbit tibial leg lengthening model. J Pediatr Orthop 2003; 23:478-483.
101. Taylor KF, Inoue N, Rafiee B, Tis JE, McHale KA, Chao EY. Effect of pulsed electromagnetic fields on maturation of regenerate bone in a rabbit limb lengthening model. J Orthop Res 2006; 24:2-10.
102. Inoue N, Ohnishi I, Chen D, Deitz LW, Schwardt JD, Chao EY. Effect of pulsed electromagnetic fields (PEMF) on late-phase osteotomy gap healing in a canine tibial model. J Orthop Res 2002; 20:1106-1114.
103. Midura RJ, Ibiwoye MO, Powell KA, Sakai Y, Doehring T, Grabiner MD, Patterson TE, Zborowski M, Wolfman A. Pulsed electromagnetic field treatments enhance the healing of fibular osteotomies. J Orthop Res 2005; 23:1035-1046.
104. Sharrard WJ, Sutcliffe ML, Robson MJ, Maceachern AG. The treatment of fibrous non-union of fractures by pulsing electromagnetic stimulation. J Bone Joint Surg Br 1982; 64:189-193.
105. Ito H, Shirai Y. The efficacy of ununited tibial fracture treatment using pulsing electromagnetic fields: relation to biological activity on nonunion bone ends. J Nippon Med Sch 2001; 68:149-153.
106. Saltzman C, Lightfoot A, Amendola A. PEMF as treatment for delayed healing of foot and ankle arthrodesis. Foot Ankle Int 2004; 25:771-773.
107. Bose B. Outcomes after posterolateral lumbar fusion with instrumentation in patients treated with adjunctive pulsed electromagnetic field stimulation. Adv Ther 2001; 18:12-20.
108. Linovitz RJ, Pathria M, Bernhardt M, Green D, Law MD, McGuire RA, Montesano PX, Rechtine G, Salib RM, Ryaby JT, Faden JS, Ponder R, Muenz LR, Magee FP, Garfin SA. Combined magnetic fields accelerate and increase spine fusion: a double-blind, randomized, placebo controlled study. Spine 2002; 27:1383-1389.
109. Mackenzie D, Veninga FD. Reversal of delayed union of anterior cervical fusion treated with pulsed electromagnetic field stimulation: case report. South Med J 2004; 97:519-524.
110. Luna Gonzalez F, Lopez Arevalo R, Meschian Coretti S, Urbano Labajos V, Delgado Rufino B. Pulsed electromagnetic stimulation of regenerate bone in lengthening procedures. Acta Orthop Belg 2005; 71:571-576.
111. Seber S, Omeroglu H, Cetinkanat H, Kose N. [The efficacy of pulsed electromagnetic fields used alone in the treatment of femoral head osteonecrosis: a report of two cases]. Acta Orthop Traumatol Turc 2003; 37:410-413. [Article in Turkish].
112. Varani K, Gessi S, Merighi S, Iannotta V, Cattabriga E, Spisani S, Cadossi R, Borea PA. Effect of low frequency electromagnetic fields on A2A adenosine receptors in human neutrophils. Br J Pharmacol 2002; 136:57-66.
113. Sollazzo V, Traina GC, DeMattei M, Pellati A, Pezzetti F, Caruso A. Responses of human MG-63 osteosarcoma cell line and human osteoblast-like cells to pulsed electromagnetic fields. Bioelectromagnetics 1997; 18:541-547.
114. Diniz P, Shomura K, Soejima K, Ito G. Effects of pulsed electromagnetic field (PEMF) stimulation on bone tissue like formation are dependent on the maturation stages of the osteoblasts. Bioelectromagnetics 2002; 23:398-405.
115. Lohmann CH, Schwartz Z, Liu Y, Guerkov H, Dean DD, Simon B, Boyan BD. Pulsed electromagnetic field stimulation of MG63 osteoblast-like cells affects differentiation and local factor production. J Orthop Res 2000; 18:637-646.
116. Lohmann CH, Schwartz Z, Liu Y, Li Z, Simon BJ, Sylvia VL, Dean DD, Bonewald LF, Donahue HJ, Boyan BD. Pulsed electromagnetic fields affect phenotype and connexin 43 protein expression in MLO-Y4 osteocyte-like cells and ROS 17/2.8 osteoblast-like cells. J Orthop Res 2003; 21:326-334.
117. Bodamyali T, Bhatt B, Hughes FJ, Winrow VR, Kanczler JM, Simon B, Abbott J, Blake DR, Stevens CR. Pulsed electromagnetic fields simultaneously induce osteogenesis and upregulate transcription of bone morphogenetic proteins 2 and 4 in rat osteoblasts in vitro. Biochem Biophys Res Commun 1998; 250:458-461.
118. Reher P, Doan N, Bradnock B, Meghji S, Harris M. Effect of ultrasound on the production of IL-8, basic FGF and VEGF. Cytokine 1999; 11:416-423.
119. Chang K, Chang WH, Tsai MT, Shih C. Pulsed electromagnetic fields accelerate apoptotic rate in osteoclasts. Connect Tissue Res 2006; 47:222-228.
120. Chang K, Chang WH, Huang S, Huang S, Shih C. Pulsed electromagnetic fields stimulation affects osteoclast formation by modulation of osteoprotegerin, RANK ligand and macrophage colony-stimulating factor. J Orthop Res 2005; 23:1308-1314.
121. Li JK, Lin JC, Liu HC, Sun JS, Ruaan RC, Shih C, Chang WH. Comparison of ultrasound and electromagnetic field effects on osteoblast growth. Ultrasound Med Biol 2006; 32:769-775.
122. Shimizu T, Zerwekh JE, Videman T, Gill K, Mooney V, Holmes RE, Hagler HK. Bone ingrowth into porous calcium phosphate ceramics: influence of pulsing electromagnetic field. J Orthop Res 1988; 6:248-258.
123. Spadaro JA, Albanese SA, Chase SE. Electromagnetic effects on bone formation at implants in the medullary canal in rabbits. J Orthop Res 1990; 8:685-693.
124. Ijiri K, Matsunaga S, Fukuyama K, Maeda S, Sakou T, Kitano M, Senba I. The effect of pulsing electromagnetic field on bone ingrowth into a porous coated implant. Anticancer Res 1996; 16:2853-2856. 125 Matsumoto H, Ochi M, Abiko Y, Hirose Y, Kaku T, Sakaguchi K. Pulsed electromagnetic fields promote bone formation around dental implants inserted into the femur of rabbits. Clin Oral Implants Res 2000; 11:354-360.
125. Fini M, Cadossi R, Cane V, Cavani F, Giavaresi G, Krajewski A, Martini L, Aldini NN, Ravaglioli A, Rimondini L, Torricelli P, Giardino R. The effect of pulsed electromagnetic fields on the osteointegration of hydroxyapatite implants in cancellous bone: a morphologic and microstructural in vivo study. J Orthop Res 2002; 20:756-763.
126. Fini M, Giavaresi G, Giardino R, Cavani F, Cadossi R. Histomorphometric and mechanical analysis of the hydroxyapatite-bone interface after electromagnetic stimulation: an experimental study in rabbits. J Bone Joint Surg Br 2006; 88:123-128.
127. Ottani V, Raspanti M, Martini D, Tretola G, Ruggeri A Jr, Franchi M, Giuliani Piccari G, Ruggeri A. Electromagnetic stimulation on the bone growth using backscattered electron imaging. Micron 2002; 33:121-125.
128. Buzza EP, Shibli JA, Barbeiro RH, Barbosa JR. Effects of electromagnetic field on bone healing around commercially pure titanium surface: histologic and mechanical study in rabbits. Implant Dent 2003; 12:182-187.
129. Steinberg GG. Reversible osteolysis. J Arthroplasty 1995; 10:556-559.
130. Konrad K, Sevcic K, Foldes K, Piroska E, Molnar E. Therapy with pulsed electromagnetic fields in aseptic loosening of total hip protheses: a prospective study. Clin Rheumatol 1996; 15:325-328.
131. Kennedy WF, Roberts CG, Zuege RC, Dicus WT. Use of pulsed electromagnetic fields in treatment of loosened cemented hip prostheses. A double-blind trial. Clin Orthop Relat Res 1993; 286:198-205.
132. Claes L, Willie B. The enhancement of bone regeneration by ultrasound. Prog Biophys Mol Biol 2007; 93:384-398.
133. Doan N, Reher P, Meghji S, Harris M. In vitro effects of therapeutic ultrasound on cell proliferation, protein synthesis, and cytokine production by human fibroblasts, osteoblasts, and monocytes. J Oral Maxillofac Surg 1999; 57:409-419.
134. Reher P, Doan N, Bradnock B, Meghji S, Harris M. Effect of ultrasound on the production of IL-8, basic FGF and VEGF. Cytokine 1999; 11:416-423.
135. Reher P, Harris M, Whiteman M, Hai HK, Meghji S. Ultrasound stimulates nitric oxide and prostaglandin E2 production by human osteoblasts. Bone 2002; 31:236-241.
136. Li JK, Chang WH, Lin JC, Ruaan RC, Liu HC, Sun JS. Cytokine release from osteoblasts in response to ultrasound stimulation. Biomaterials 2003; 24:2379-2385.
137. Takayama T, Suzuki N, Ikeda K, Shimada T, Suzuki A, Maeno M, Otsuka K, Ito K. Low-intensity pulsed ultrasound stimulates osteogenic differentiation in ROS 17/2.8 cells. Life Sci 2007; 80:965-971.
138. Schumann D, Kujat R, Zellner J, Angele MK, Nerlich M, Mayr E, Angele P. Treatment of human mesenchymal stem cells with pulsed low intensity ultrasound enhances the chondrogenic phenotype in vitro. Biorheology 2006; 43:431-443.
139. Duarte LR. The stimulation of bone growth by ultrasound. Arch Orthop Trauma Surg 1983; 101:153-159.
140. Dyson M, Brookes M. Stimulation of bone repair by ultrasound. Ultrasound Med Biol 1983; (Suppl.2):61-66.
141. Yang KH, Parvizi J, Wang SJ, Lewallen DG, Kinnick RR, Greenleaf JF, Bolander ME. Exposure to low-intensity ultrasound increases aggrecan gene expression in a rat femur fracture model. J Orthop Res 1996; 14:802-809.
142. Rawool NM, Goldberg BB, Forsberg F, Winder AA, Hume E. Power Doppler assessment of vascular changes during fracture treatment with low-intensity ultrasound. J Ultrasound Med 2003; 22:145-153.
143. Eberson CP, Hogan KA, Moore DC, Ehrlich MG. Effect of low-intensity ultrasound stimulation on consolidation of the regenerate zone in a rat model of distraction osteogenesis. J Pediatr Orthop 2003; 23:46-51.
144. Claes L, Ruter A, Mayr E. Low-intensity ultrasound enhances maturation of callus after segmental transport. Clin Orthop Relat Res 2005; 430:189-194.
145. Sakurakichi K, Tsuchiya H, Uehara K, Yamashiro T, Tomita K, Azuma Y. Effects of timing of low-intensity pulsed ultrasound on distraction osteogenesis. J Orthop Res 2004; 22:395-403.
146. Chan CW, Qin L, Lee KM, Cheung WH, Cheng JC, Leung KS. Dose-dependent effect of low-intensity pulsed ultrasound on callus formation during rapid distraction osteogenesis. J Orthop Res 2006; 24:2072-2079.
147. Ter Haar G. Therapeutic applications of ultrasound. Prog Biophys Mol Biol 2007; 93:111-129.
148. Wang CJ, Chen HS, Chen CE, Yang KD. Treatment of nonunions of long bone fractures with shock waves. Clin Orthop Relat Res 2001; 387:95-101.
149. Heckman JD, Ryaby JP, McCabe J, Frey JJ, Kilcoyne RF. Acceleration of tibial fracture-healing by non-invasive, low-intensity pulsed ultrasound. J Bone Joint Surg Am 1994; 76:26-34.
150. Kristiansen TK, Ryaby JP, McCabe J, Frey JJ, Roe LR. Accelerated healing of distal radial fractures with the use of specific, low-intensity ultrasound. A multicenter, prospective, randomized, double-blind, placebo-controlled study. J Bone Joint Surg Am 1997; 79:961-973.
151. Mayr E, Rudzki MM, Rudzki M, Borchardt B, Hausser H, Ruter A. [Does low intensity, pulsed ultrasound speed healing of scaphoid fractures?] Handchir Mikrochir Plast Chir 2000; 32:115-122. [Article in German].
152. Emami A, Petren-Mallmin M, Larsson S. No effect of low-intensity ultrasound on healing time of intramedullary fixed tibial fractures. J Orthop Trauma 1999; 13:252-257.
153. Mayr E, Wagner S, Ecker M, Ruter A. [Ultrasound therapy for nonunions. Three case reports.] Unfallchirurg 1999; 102:191-196. [Article in German].
154. Nolte PA, van der Krans A, Patka P, Janssen IM, Ryaby JP, Albers GH. Low-intensity pulsed ultrasound in the treatment of nonunions. J Trauma 2001; 51:693-702.
155. Gebauer D, Correll J. Pulsed low-intensity ultrasound: a new salvage procedure for delayed unions and nonunions after leg lengthening in children. J Pediatr Orthop 2005; 25:750-754.
156. Gebauer D, Mayr E, Orthner E, Ryaby JP. Low-intensity pulsed ultrasound: effects on nonunions. Ultrasound Med Biol 2005; 31:1391-1402.
157. Scott G, King JB. A prospective, double-blind trial of electrical capacitive coupling in the treatment of non-union of long bones. J Bone Joint Surg Am 1994; 76:820-826.
158. Wang CJ, Chen HS, Chen CE, Yang KD. Treatment of nonunions of long bone fractures with shock waves. Clin Orthop Relat Res 2001; 387:95-101.
159. Okada K, Miyakoshi N, Takahashi S, Ishigaki S, Nishida J, Itoi E. Congenital pseudoarthrosis of the tibia treated with low-intensity pulsed ultrasound stimulation (LIPUS). Ultrasound Med Biol 2003; 29:1061-1064.
160. Qin L, Lu H, Fok P, Cheung W, Zheng Y, Lee K, Leung K. Low-intensity pulsed ultrasound accelerates osteogenesis at bone-tendon healing junction. Ultrasound Med Biol 2006; 32:1905-1911.
161. Welgus HG, Jeffrey JJ, Eisen AZ, Roswit WT, Stricklin GP. Human skin fibroblast collagenase: interaction with substrate and inhibitor. Coll Relat Res 1985; 5:167-179.
162. Lin FH, Lin CC, Lu CM, Liu HC, Wang CY. The effects of ultrasonic stimulation on DP-bioglass bone substitute. Med Eng Phys 1995; 17:20-26.
163. Tanzer M, Harvey E, Kay A, Morton P, Bobyn JD. Effect of noninvasive low intensity ultrasound on bone growth into porous-coated implants. J Orthop Res 1996; 14:901-906.
164. Tanzer M, Kantor S, Bobyn JD. Enhancement of bone growth into porous intramedullary implants using non-invasive low intensity ultrasound. J Orthop Res 2001; 19:195-199.