Development of a new antibody to the human inhibin/activin βB subunit and its application to improved inhibin B ELISAs

Journal of Immunological Methods

Volumn 329, Issue 1-2, 2008, Pages 102-111

  Ludlow, Helen ^a   Muttukrishna, Shanthi ^b   Hyvönen, Marko ^c   Groome, Nigel P ^a  

^a OXFORD BROOKES UNIVERSITY   (United Kingdom)

^b ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Activin B; ELISA; Immunoassay; Inhibin B; Monoclonal antibodies; RIMMS

Indexed keywords

ACTIVIN; EPITOPE; HYDROGEN PEROXIDE; INHIBIN; METHIONINE; MONOCLONAL ANTIBODY; MYELOPID; RECOMBINANT PROTEIN; SULFOXIDE;

ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ARTICLE; CROSS REACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNIZATION; IMMUNOASSAY; IMMUNOREACTIVITY; MOUSE; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTEIN FUNCTION; REPRODUCTION; XENOPUS LAEVIS;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL LINE; CROSS REACTIONS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMANS; INHIBIN-BETA SUBUNITS; INHIBINS; MALE; MICE; MIDDLE AGED; POSTMENOPAUSE; REAGENT KITS, DIAGNOSTIC; RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; XENOPUS PROTEINS;

MUS; XENOPUS LAEVIS;

EID: 36849034860     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.09.013     Document Type: Article

References (43)

1. Andersen D.C., Koch C., Jensen C.H., Skjodt K., Brandt J., and Teisner B. High prevalence of human anti-bovine IgG antibodies as the major cause of false positive reactions in two-site immunoassays based on monoclonal antibodies. J. Immunoass. Immunochem. 25 (2004) 17
2. Attardi B., Vaughan J., and Vale W. Regulation of FSH beta messenger ribonucleic acid levels in the rat by endogenous inhibin. Endocrinology 130 (1992) 557
3. Burger H.G., and Igarashi M. Inhibin: definition and nomenclature, including related substances. J. Clin. Endocrinol. Metab. 66 (1988) 885
4. Burger H.G., Groome N.P., and Robertson D.M. Both inhibin A and B respond to exogenous follicle-stimulating hormone in the follicular phase of the human menstrual cycle. J. Clin. Endocrinol. Metab. 83 (1998) 4167
5. Caterson B., Christner J.E., and Baker J.R. Identification of a monoclonal antibody that specifically recognizes corneal and skeletal keratan sulfate. Monoclonal antibodies to cartilage proteoglycan. J. Biol. Chem. 258 (1983) 8848
6. Evans L.W., and Groome N.P. Development of immunoassays for inhibin, activin and follistatin. In: Muttukrishna S., and Ledger W. (Eds). Inhibin, Activin and Follistatin in Human Reproductive Physiology (2001), Imperial College Press, London 11
7. Fowers K.D., Callahan J., Byron P., and Kopecek J.I. Preparation of Fab' from murine IgG2a for thiol reactive conjugation. J. Drug Target 9 (2001) 281
8. Groome N., Hancock J., Betteridge A., Lawrence M., and Craven R. Monoclonal and polyclonal antibodies reactive with the 1-32 amino terminal sequence of the alpha subunit of human 32K inhibin. Hybridoma 9 (1990) 31
9. Groome N.P., Illingworth P.J., O'Brien M., Pai R., Rodger F.E., Mather J.P., and McNeilly A.S. Measurement of dimeric inhibin B throughout the human menstrual cycle. J. Clin. Endocrinol. Metab. 81 (1996) 1401
10. Harlow E., and Lane D. Immunoaffinity purification. Antibodies: a laboratory manual (1988), Cold Spring Harbour Laboratory Press, New York 511
11. Illingworth P.J., Groome N.P., Byrd W., Rainey W.E., McNeilly A.S., Mather J.P., and Bremner W.J. Inhibin-B: a likely candidate for the physiologically important form of inhibin in men. J. Clin. Endocrinol. Metab. 81 (1996) 1321
12. Ismail A.A., Walker P.L., Barth J.H., Lewandowski K.C., Jones R., and Burr W.A. Wrong biochemistry results: two case reports and observational study in 5310 patients on potentially misleading thyroid-stimulating hormone and gonadotropin immunoassay results. Clin. Chem. 48 (2002) 2023
13. Kishiro Y., Kagawa M., Naito I., and Sado Y. A novel method of preparing rat-monoclonal antibody-producing hybridomas by using rat medial iliac lymph node cells. Cell Struct. Funct. 20 (1995) 151
14. Knight P.G., and Glister C. Potential local regulatory functions of inhibins, activins and follistatin in the ovary. Reproduction 121 (2001) 503
15. Knight P.G., and Muttukrishna S. Measurement of dimeric inhibin using a modified two-site immunoradiometric assay specific for oxidized (Met O) inhibin. J. Endocrinol. 141 (1994) 417
16. Knight P.G., Muttukrishna S., and Groome N.P. Development and application of a two-site enzyme immunoassay for the determination of 'total' activin-A concentrations in serum and follicular fluid. J. Endocrinol. 148 (1996) 267
17. Kricka L.J. Human anti-animal antibody interferences in immunological assays. Clin. Chem. 45 (1999) 942
18. Lambert-Messerlian G., Bandera C., Eklund E., Neuhauser A., and Canick J. Very high inhibin A concentration attributed to heterophilic antibody interference. Clin. Chem. 53 (2007) 800
19. Lamoyi E. Preparation of F(ab')2 fragments from mouse IgG of various subclasses. Methods Enzymol. 121 (1986) 652
20. Ling N., Ying S.Y., Ueno N., Shimasaki S., Esch F., Hotta M., and Guillemin R. A homodimer of the beta-subunits of inhibin A stimulates the secretion of pituitary follicle stimulating hormone. Biochem. Biophys. Res. Commun. 138 (1986) 1129
21. Marks V. False-positive immunoassay results: a multicenter survey of erroneous immunoassay results from assays of 74 analytes in 10 donors from 66 laboratories in seven countries. Clin. Chem. 48 (2002) 2008
22. Mason A.J., Niall H.D., and Seeburg P.H. Structure of two human ovarian inhibins. Biochem. Biophys. Res. Commun. 135 (1986) 957
23. Milenic D.E., Esteban J.M., and Colcher D. Comparison of methods for the generation of immunoreactive fragments of a monoclonal antibody (B72.3) reactive with human carcinomas. J. Immunol. Methods 120 (1989) 71
24. Mirza I.H., Wilkin T.J., Cantarini M., and Moore K. A comparison of spleen and lymph node cells as fusion partners for the raising of monoclonal antibodies after different routes of immunisation. J. Immunol. Methods 105 (1987) 235
25. Miyamoto K., Hasegawa Y., Fukuda M., Nomura M., Igarashi M., Kangawa K., and Matsuo H. Isolation of porcine follicular fluid inhibin of 32K Daltons. Biochem. Biophys. Res. Commun. 129 (1985) 396
26. Morgan B.R., and Tarter T.H. Serum heterophile antibodies interfere with prostate specific antigen test and result in over treatment in a patient with prostate cancer. J. Urol. 166 (2001) 2311
27. Muttukrishna S., Child T., Lockwood G.M., Groome N.P., Barlow D.H., and Ledger W.L. Serum concentrations of dimeric inhibins, activin A, gonadotrophins and ovarian steroids during the menstrual cycle in older women. Hum. Reprod. 15 (2000) 549
28. O'Fegan P. Validation. In: Gosling J.P. (Ed). Immunoassays (2000), Oxford University Press, Oxford 211
29. Pearson W.R., Wood T., Zhang Z., and Miller W. Comparison of DNA sequences with protein sequences. Genomics 46 (1997) 24
30. Robertson D.M., Foulds L.M., Leversha L., Morgan F.J., Hearn M.T., Burger H.G., Wettenhall R.E., and de Kretser D.M. Isolation of inhibin from bovine follicular fluid. Biochem. Biophys. Res. Commun. 126 (1985) 220
31. Robertson D.M., Stephenson T., Pruysers E., McCloud P., Tsigos A., Groome N., Mamers P., and Burger H.G. Characterization of inhibin forms and their measurement by an inhibin alpha-subunit ELISA in serum from postmenopausal women with ovarian cancer. J. Clin. Endocrinol. Metab. 87 (2002) 816
32. Robertson D.M., Burger H.G., and Fuller P.J. Inhibin/activin and ovarian cancer. Endocr. Relat. Cancer 11 (2004) 35
33. Robertson D.M., Pruysers E., Burger H.G., Jobling T., McNeilage J., and Healy D. Inhibins and ovarian cancer. Mol. Cell. Endocrinol. 225 (2004) 65
34. Sado Y., Inoue S., Tomono Y., and Omori H. Lymphocytes from enlarged iliac lymph nodes as fusion partners for the production of monoclonal antibodies after a single tail base immunization attempt. Acta Histochem. Cytochem. 39 (2006) 89
35. Vale W., Rivier J., Vaughan J., McClintock R., Corrigan A., Woo W., Karr D., and Spiess J. Purification and characterization of an FSH releasing protein from porcine ovarian follicular fluid. Nature 321 (1986) 776
36. Vale W., Rivier C., Hsueh A., Campen C., Meunier H., Bicsak T., Vaughan J., Corrigan A., Bardin W., Sawchenko P., and e.al. Chemical and biological characterization of the inhibin family of protein hormones. Recent Prog. Horm. Res. 44 (1988) 1
37. Wald N.J., Huttly W.J., and Hackshaw A.K. Antenatal screening for Down's syndrome with the quadruple test. Lancet 361 (2003) 835
38. Wallace E.M., Crossley J.A., Ritoe S.C., Aitken D.A., Spencer K., and Groome N.P. Evolution of an inhibin A ELISA method: implications for Down's syndrome screening. Ann. Clin. Biochem. 35 (1998) 656
39. Weber T.H., Kapyaho K.I., and Tanner P. Endogenous interference in immunoassays in clinical chemistry. A review. Scand. J. Clin. Lab. Invest. Suppl. 201 (1990) 77
40. Wong W.L., Garg S.J., Woodruff T., Bald L., Fendly B., and Lofgren J.A. Monoclonal antibody based ELISAs for measurement of activins in biological fluids. J. Immunol. Methods 165 (1993) 1
41. Woodruff T.K., Besecke L.M., Groome N., Draper L.B., Schwartz N.B., and Weiss J. Inhibin A and inhibin B are inversely correlated to follicle-stimulating hormone, yet are discordant during the follicular phase of the rat estrous cycle, and inhibin A is expressed in a sexually dimorphic manner. Endocrinology 137 (1996) 5463
42. Wring S.A., Kilpatrick K.E., Hutchins J.T., Witherspoon S.M., Ellis B., Jenner W.N., and Serabjit-Singh C. Shorter development of immunoassay for drugs: application of the novel RIMMS technique enables rapid production of monoclonal antibodies to ranitidine. J. Pharm. Biomed. Anal. 19 (1999) 695
43. Yamaguchi Y., Kim H., Kato K., Masuda K., Shimada I., and Arata Y. Proteolytic fragmentation with high specificity of mouse immunoglobulin G. Mapping of proteolytic cleavage sites in the hinge region. J. Immunol. Methods 181 (1995) 259