Preparation of Fab' from murine IgG2a for thiol reactive conjugation

Journal of Drug Targeting

Volumn 9, Issue 4, 2001, Pages 281-294

  Fowers, K D ^a   Callahan, J ^a   Byron, P ^a   Kopecek J I ^a  

^a Department of Electrical and Computer Engineering   (United States)

Author keywords

Fab' fragment; HPMA copolymer; Lysyl endopeptidase; Pepsin; Protein G; Targeting

Indexed keywords

AMIDE; BUTYLOXYCARBONYLVALYLLEUCYLLYSYL 7 AMIDO 4 METHYLCOUMARIN; COUMARIN DERIVATIVE; GLYCOPROTEIN P; IMMUNOGLOBULIN F(AB')2 FRAGMENT; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G2A; LYSINE; LYSYL PROTEINASE; MALEIMIDE; MESO CHLORIN E6 MONO (N2 AMINOETHYLAMIDE); MONOCLONAL ANTIBODY; N (2 HYDROXYPROPYL)METHACRYLAMIDE COPOLYMER; PEPSIN A; PROTEIN ANTIBODY; PROTEIN G; PROTEINASE; THIOL; UNCLASSIFIED DRUG;

ACHROMOBACTER; ACHROMOBACTER LYTICUS; ARTICLE; CARCINOMA CELL; CELL SURVIVAL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; DRUG CONJUGATION; DRUG TARGETING; ENZYME ACTIVITY; HUMAN; HUMAN CELL; HYBRIDOMA; IMMUNOAFFINITY CHROMATOGRAPHY; IONIC STRENGTH; MONITORING; MOUSE; NONHUMAN; OVARY CARCINOMA; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PURIFICATION;

EID: 0034887156     PISSN: 1061186X     EISSN: None     Source Type: Journal    
DOI: 10.3109/10611860108997936     Document Type: Article

References (48)

1. 6 photodynamic activity in vitro with peptide-based intracellular vehicles
2. Preparation, phototoxicity and biodistribution studies of anti-carcinoembryonic antigen monoclonal antibody-phthalocyanine conjugates
3. 2 fragments
4. Solid phase synthesis of bifunctional antibodies
5. Structural and functional analysis of the human IgG-Fab receptor activity of streptococcal protein G
6. Photoreduction of monoclonal antibodies for conjugation and fragmentation
7. 2-mediated immunoglobulin binding to group C and G streptococci
8. 2 binding to group C and G streptococci is mediated by structures on gamma chains
9. Streptococcal protein G has affinity for both Fab- and Fc-fragments of human IgG
10. Definition of Type I and Type II photosensitized oxidation
11. 2b-producing mouse myeloma cell line. Structural studies on the Fc region of parent and variant heavy chains
12. 2 fragment of a murine monoclonal antibody using capillary isoelectric focusing and electrospray ionization mass spectrometry
13. Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill
14. Immunocytochemistry by electron spectroscopic imaging using well defined boronated monovalent antibody fragments
15. O-glycosylation in hinge region of mouse immunoglobulin G2b
16. Dynamical structure of the hinge region of immunoglobulin G as studied by 13C nuclear magnetic resonance spectroscopy
17. HPMA copolymer - Anticancer drug conjugates: Design, activity, and mechanism of action
18. Mechanisms of ligand binding by monoclonal anti-fluorescyl antibodies
19. A polymeric drug delivery system for the simultaneous delivery of drugs activatable by enzymes and/or light
20. Radiation survival parameters of antineoplastic drug-sensitive and -resistant human ovarian cancer cell lines and their modification by buthionine sulfoximine
21. Polymerizable Fab' antibody fragments for targeting of anticancer drugs
22. 2 suitable for clinical use from mouse IgG1
23. HPMA copolymer-anticancer drug-OV-TL16 antibody conjugates. 1. Influence of the method of synthesis on the binding affinity to OVCAR-3 ovarian carcinoma cells in vitro
24. HPMA copolymer-anticancer drug-OV-TL16 antibody conjugates. II. Processing in epithelial ovarian carcinoma cells in vitro
25. On the fragmentation of monoclonal IgG1, IgG2a, and IgG2b from BALB/c mice
26. Automated assay for HER-2/neu in serum
27. Combination chemotherapy and photodynamic therapy with N-(2-hydroxypropyl)methacrylamide copolymer-bound anticancer drugs inhibit human ovarian carcinoma heterotransplanted in nude mice
28. Structure determination of a monoclonal Fab fragment specific for histidine-containing protein of the phosphoenolpyruvate: Sugar phosphotransferase system of Escherichia coli
29. 2 using streptococcal protein G
30. Polymer Conjugates with Anti-cancer Activity
31. A novel method for controlling the pepsin digestion of antibodies
32. Aminolyses of monomeric and polymeric 4-nitrophenyl esters of N-methacryloylamino acids
33. Antibody directed affinity therapy applied to the immune system: In vivo effectiveness and limited toxicity of daunomycin conjugates to HPMA copolymers and targeting antibody
34. 2 - Targeted conjugates and combined therapy with immunomodulators
35. Lysyl endopeptidase of Achrornobacter lyticus
36. 6 and adriamycin in nude mice bearing human ovarian carcinoma OVCAR-3 xenografts
37. 6 and adriamycin conjugates in combination treatments
38. New trends in photobiology. Chlorins as photosensitizers in biology and medicine
39. The effect of acid treatment upon the susceptibility of rabbit IgG to proteolytic cleavage with various enzymes
40. Binding parameters and idiotypic profile of the whole immunoglobulin and Fab' fragments of murine monoclonal antibody to distinct determinants of the human high molecular weight-melanoma associated antigen
41. 6 does not induce P-glycoprotein-mediated multidrug resistance
42. The primary structure and structural characteristics of Achromobacter lyticus protease I, a lysine-specific serine protease
43. Tumor imaging with technetium-99m-labeled hydrazinonicotinamide-Fab' conjugates
44. A versatile method for the conjugation of proteins and peptides to poly[2-(dimethylamino)ethyl methacrylate]
45. Proteolytic fragmentation with high specificity of mouse immunoglobulin G. Mapping of proteolytic cleavage sites in the hinge region
46. A new fluorogenic substrate for chymotrypsin