High-yield expression and purification of isotopically labeled cytochrome P450 monooxygenases for solid-state NMR spectroscopy

Biochimica et Biophysica Acta - Biomembranes

Volumn 1768, Issue 12, 2007, Pages 3061-3070

  Rupasinghe, Sanjeewa G ^a   Duan, Hui ^a   Frericks Schmidt, Heather L ^b   Berthold, Deborah A ^b   Rienstra, Chad M ^b   Schuler, Mary A ^a,b  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Cytochrome P450 monooxygenases (P450s); Heterologous expression; Membrane protein; Solid state NMR analysis

Indexed keywords

CARBON 13; CHAPERONIN; CYP98A3 ENZYME; CYTOCHROME P450; HISTONE; NITROGEN 15; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

ARABIDOPSIS; ARTICLE; ENZYME ANALYSIS; ENZYME MODIFICATION; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; EUKARYOTE; ISOTOPE LABELING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; X RAY CRYSTALLOGRAPHY;

CARBON ISOTOPES; CYTOCHROME P-450 ENZYME SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; MAGNETIC RESONANCE SPECTROSCOPY; NITROGEN ISOTOPES; RECOMBINANT PROTEINS;

ARABIDOPSIS; ESCHERICHIA COLI; EUKARYOTA; HEXAPODA;

EID: 36849003927     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbamem.2007.09.009     Document Type: Article

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