Differentiation of hake species by RFLP- and SSCP-analysis of PCR amplified cytochrome b and paralbumin sequences

Deutsche Lebensmittel-Rundschau

Volumn 103, Issue 11, 2007, Pages 511-517

  Rehbein, Hartmut ^a  

^a FEDERAL RESEARCH INSTITUTE OF NUTRITION AND FOOD   (Germany)

Author keywords

Hake; PCR; RFLP; Species identification; SSCP

Indexed keywords

MERLUCCIUS; MERLUCCIUS ALBIDUS; MERLUCCIUS AUSTRALIS; MERLUCCIUS BILINEARIS; MERLUCCIUS CAPENSIS; MERLUCCIUS POLLI; MERLUCCIUS PRODUCTUS; REXEA SOLANDRI;

EID: 36649038739     PISSN: 00120413     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. Albeit, J. and T. J. Pitcher (eds.): Hake-Fisheries, Ecology and Markets. Chapman and Hall, London (1995).
2. Anonymous: Schlemmerfillets. Test (2), 16-22 (2006).
3. Barros, F, M. V. Lareu, A. Salas and A. Carracedo: Rapid and enhanced detection of mitochondrial DNA variation using single-strand conformation analysis of superposed restriction enzyme fragments from polymerase chain reaction amplified products. Electrophoresis 18, 52-54 (1997).
4. Downs, T. R. and W. W. Wilfinger. Fluorometric quantification of DNA in cells and tissues. Anal Biochem 131, 538-547 (1983).
5. Hold, G. L., V. J. Russell, S. E. Pryde, H. Rehbein, J. Quinteiro, R. Vidal, M. Rey-Mendez, C. G. Sotelo, R. I. Perez-Martin, A. T. Santos and C. Rosa: Development of a DNA-based method aimed at identifying the fish species present in food products. J Agr Food Chem 49, 1175-1179 (2001).
6. Lloris, D. and J. Matallanas: Description of a new species of hake: Merluccius patagonicus sp. nov. (Gadiformes: Merluciidae) from the waters of Argentina. Scientia Marina 67, 323-326 (2003).
7. Matallanas, J. and D. Lloris: Description of Merluccius tasmanicus sp. nov. and redescription of Merluccius australis (Pisces: Merluciidae). J Marine Biology Association of the United Kingdom 86, 193-199 (2006).
8. Martins, A., M. R. Bronze, I. Batista and M. L. Nunes: Evaluation of the quality of hake products during frozen storage. In: Rehbein, H., H. Karl, M. Manthey-Karl, J. Oehlenschläger and R Schubring (eds.): Proceedings of the 34th WEFTA Conference, pp. 114-116. Federal Research Centre for Nutrition and Food, Hamburg (2004).
9. Perez, J. and E. Garcia-Vazquez. Genetic identification of nine hake species for detection of commercial fraud. J Food Protect 67, 2792-2796 (2004).
10. Perez, M., J. M. Vieites and P Presa: ITS1-rDNA-based methodology to identify world-wide hake species of the genus Merluccius. J Agr Food Chem 53, 5239-5247 (2005).
11. Quinteiro, J., Vidal, R., Izquierdo, M., Sotelo, C.G., Chapela, M.J., Perez-Martin, R., Rehbein, H., Hold, G.L., Russell, V. J., S. E. Pryde, C. Rosa, A. T. Santos and M. Rey-Mendez: Identification of hake species (Merluccius genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences. J Agr Food Chem 49, 5106-5114 (2001).
12. Rehbein, H.: Identification of the fish species processed to fish meal. J Aquatic Food Prod Technol 11, 45-56 (2002).
13. Rehbein, H. and G. Kreß: Detection of short mRNA sequences in fishery products. Deut Lebensm-Rundsch 101, 333-337 (2005).
14. Sunnucks, P., A. C. C. Wilson, B. Beheregaray and K. Zenger. SSCP is not so difficult: the application and utility of single-stranded conformation polymorphism in evolutionary biology and molecular ecology. Mol Ecol 9, 1699-1710 (2000).
15. Teschauer, W., T Mussack, A. Braun, H. Waldner and E. Fink. Conditions for single strand conformation polymorphism (SSCP) analysis with broad applicability: a study on the effects of acrylamide, buffer and glycerol concentrations in SSCP analysis of exons of the p53 gene. Eur J Clin Chem Clin Biochem 34, 125-131 (1996).
16. Swoboda, I., A. Bugajska-Schretter, P. Verdino, W. Keller, W. R. Sperr, P. Valent, R. Valenta and S. Spitzauer: Recombinant carp parvalbumin, the major cross-reactive fish allergen: a tool for diagnosis and therapy of fish allergy. The Journal of Immunology 168, 4576-4584 (2002).
17. Wolf, C., M. Burgener, P Hübner and J. Lüthy. PCR-RFLP analysis of mitochondrial DNA: differentiation of fish species. Food Sci Technol-Lebensm Wiss 33, 144-150 (2000).