SSCP is not so difficult: The application and utility of single-stranded conformation polymorphism in evolutionary biology and molecular ecology

Molecular Ecology

Volumn 9, Issue 11, 2000, Pages 1699-1710

  Sunnucks, Paul ^a,b   Wilson, A C C ^a,c   Beheregaray, L B ^a   Zenger, K ^a   French, J ^a   Taylor, A C ^a,d  

^a MACQUARIE UNIVERSITY   (Australia)

^b LA TROBE UNIVERSITY   (Australia)

^c AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

^d MONASH UNIVERSITY   (Australia)

Author keywords

Allele genealogies; DNA sequence; PCR; Population genetics; SSCP

Indexed keywords

DNA DETERMINATION; GENETIC VARIANCE; NUCLEOTIDE SEQUENCE; SINGLE STRAND CONFORMATION POLYMORPHISM;

ANIMALS; BASE SEQUENCE; DNA; DNA PRIMERS; DNA, MITOCHONDRIAL; ECOLOGY; EVOLUTION, MOLECULAR; GENETICS, POPULATION; MICROSATELLITE REPEATS; MULTIGENE FAMILY; PHYLOGENY; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; PSEUDOGENES; VARIATION (GENETICS);

EID: 0033730043     PISSN: 09621083     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-294X.2000.01084.x     Document Type: Article

References (70)

1. Intensive linkage mapping in a wasp (Bracon hebetor) and a mosquito (Aedes aegypti) with single-strand conformation polymorphism analysis of Random Amplified Polymorphic DNA markers
2. Double-stranded conformation polymorphism (DSCP) analysis of the mitochondrial control region generates high variable markers for population studies in a social insect
3. Mitochondrial and nuclear sequence variability among populations of rainbow trout (Oncorhynchus mykiss)
4. Polymorphic molecular markers from anonymous nuclear DNA for genetic analysis of populations
5. Microsatellite loci isolated from Odontesthes argentinensis and the O. perugiae species group and their use in other South American silverside fish
6. Molecular markers reveal cryptic sex in the human pathogen Coccidioides immitis
7. DNA sequencing with arbitrary primer pairs
8. Genetic architecture of physiological phenotypes: Empirical evidence for coadapted gene complexes
9. Outgroup heteroduplex analysis using temperature gradient gel electrophoresis: High resolution, large scale, screening of DNA variation in the mitochondrial control region
10. Mechanisms of population differentiation in marbled murrelets: Historical vs contemporary evolutionary processes
11. Preserving the information content of species - Genetic diversity, phylogeny, and conservation worth
12. Molecular anatomy of an onychophoran: Compartmentalized sperm storage and heterogeneous paternity
13. Microsatellite evolution: Inferences from population data
14. Mutation detection by denaturing gradient electrophoresis (DGGE)
15. Intron variation in marbled murrelets detected using analysis of single-stranded conformational polymorphism
16. Mutation scanning methods for the analysis of parasite genes
17. Screening for nucleotide variations in ribosomal DNA arrays of Oesophagostomum bifurcum by polymerase chain reaction-coupled single-strand conformation polymorphism
18. Amplification of anonymous DNA fragments using pairs of long primers generates reproducible DNA fingerprints that are sensitive to genetic variation
19. The use of PCR-based single-stranded conformation polymorphism analysis (SSCP-PCR) in conservation genetics
20. PCR-SSCP: A simple and sensitive method for detection of mutations in the genomic DNA
21. PCR-SSCP: A method for detection of mutations
22. How sensitive is PCR-SSCP?
23. Major histocompatability complex variation in the endangered Przewalski's horse
24. Use of DNA toolbox for the characterization of mutation scanning methods. II: Evaluation of single-strand conformation polymorphism analysis
25. 'Cold SSCP': A simple, rapid and non-radioactive method for optimized single-strand conformation polymorphism analyses
26. Polymerase chain reaction - Single strand conformation polymorphism or how to detect reliably and efficiently each sequence variation in many samples
27. +-transporting ATPase: Determination of altered sites of the structural gene
28. Dynamics of mitochondrial DNA evolution in animals: Amplification and sequencing with conserved primers
29. Screening of Mhc variation in Atlantic salmon (Salmo salar): A comparison of restriction fragment length polymorphism (RFLP), denaturing gradient gel electrophoresis (DGGE) and sequencing
30. Structure and evolution of teleost mitochondrial control region
31. Screening techniques for detecting allelic variation in DNA sequences
32. Detection of virually all mutations-SSCP (DOVAM-S): A rapid method for mutation scanning with virtually 100% sensitivity
33. Comparative phylogeography and the identification of genetically divergent areas for conservation
34. Molecular Ecology: Contributions from molecular genetics to population ecology
35. Allelic and haplotype variation of major histocompatibility complex class II DRB1 and DQB loci in the St. Lawrence beluga (Delphinapterus leucas)
36. Single-strand conformation polymorphism and heteroduplex analysis for gel-based mutation detection
37. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms
38. Rapid and sensitive detection of point mutations and DNA polymorphisms using the polymerase chain reaction
39. Detection and isolation of nuclear haplotypes by PCR-SSCP
40. Phylogenetic assessment of length variation at a microsatellite locus
41. Amplifying ancient DNA
42. Nucleic Acids II: The polymerase chain reaction
43. Review of the Peripatopsidae (Onychophora) in Australia, with comments on peripatopsid relationships
44. Reproductive mode and population genetic structure of the cereal aphid Sitobion avenae studied using phenotypic and microsatellite markers
45. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers
46. Rapid assessment of single-copy nuclear DNA variation in diverse species
47. Species relationships in population structure of Littorina saxatilis Olivi and L. tenebrosa Montagu in Ireland using single-strand conformational polymorphisms (SSCPs) of cytochrome b fragments
48. Major histocompatibility complex (MHC) class II polymorphism and paterntiy in the monogamous Hypogeomys antimena, the endangered, largest endemic Malagasy rodent
49. Heteroplasmy of mitochondrial DNA in the ophiuroid. Astrobranchion Contrictum
50. Efficient genetic markers for population biology
51. Reproductive biology of the onychophoran Euperipatoides Rowelli
52. Microsatellite and chromosome evolution of parthenogenetic Sitobion aphids in Australia
53. Numerous transposed sequences of mitochondrial cytochrome oxidase I-II in aphids of the genus Sitobion (Hemiptera: aphididae)
54. Microsatellite markers for the onychophoran Euperipatoides rowelli
55. A set of tammar wallaby (Macropus eugenii) microsatellites tested for genetic linkage
56. Retention of reproductive barriers and reproductive synchronisation in introduced sympatric Macropus spp. in New Zealand
57. The role of molecular genetics in speciation studies
58. Extreme divergence of mitochondrial DNA within species of pulmonate land snails
59. Lens crystallins of invertebrates - Diversity and recruitment from detoxification enzymes and novel proteins
60. Sequence data suggest that phylogeographic pattern in the alpine scree weta (Orthoptera, Anostostomatidae) results from late Pliocene vicariance
61. Rapid identification of bacteria from positive blood cultures by fluorescence-based PCR-single-strand conformation polymorphism analysis of the 16S rRNA gene
62. Invasion genetics of the Mediterreanean fruit fly: Variation in multiple nuclear introns
63. Microevolution, low clonal diversity and genetic affinities of parthenogenetic Sitobion aphids in New Zealand
64. Improvement of SSCP analysis by use of denaturants
65. Nuclear integrations: Challenges for mitochondrial DNA markers
66. Examination of two years of community dynamics in an anaerobic bioreactor using fluorescence polymerase chain reaction (PCR) single-strand conformation polymorphism analysis