Anthocyanin expression in marker free transgenic wheat and triticale embryos

In Vitro Cellular and Developmental Biology - Plant

Volumn 43, Issue 5, 2007, Pages 429-435

  Doshi, K M ^a   Eudes, F ^a   Laroche, A ^a   Gaudet, D ^a  

^a LETHBRIDGE RESEARCH CENTRE   (Canada)

Author keywords

Embryo specific promoter; Identity preservation; qRT PCR; Stable transformation; SYBR green method

Indexed keywords

TRITICOSECALE; TRITICUM AESTIVUM;

EID: 36649037367     PISSN: 10545476     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11627-007-9089-7     Document Type: Article

References (41)

1. Altpeter F, Baisakh N, Beachy R, Bock R, Capell T, Christou P, Daniell H, Datta K, Datta S, Dix PJ, Fauquet C, Huang N, Kohli A, Mooibroek H, Nicholson L, Nguyen TT, Nugent G, Raemakers K, Romano A, Somers DA, Stoger E, Taylor N, Visser R (2005) Particle bombardment and the genetic enhancement of crops: myths and realities. Mol Breed 15:305-327
2. Alvarez ML, Guelman NG, Halford NG, Lustig S, Reggiardo MI, Ryabushkina N, Shewry P, Stein J, Vallejos RH (2000) Silencing of HMW glutenins in transgenic wheat expressing extra HMW subunits. Theor Appl Genet 100:319-327
3. Applied Biosystems (2003). Creating standard curves with genomic DNA or plasmid DNA templates for use in quantitative PCR. Also available online, http://www.appliedbiosystems.com/support/tutorials/pdf/quant_pcr.pdf, pp. 1-9
4. Becker D, Brettschneider R, Lorz H (1994) Fertile transgenic wheat from microprojectile bombardment of scutellar tissue. Plant J 5:299-307
5. Bennett MD, Leitch IJ (2004) Plant DNA C-values database (release 3.0, Dec. 2004) http://www.rbgkew.org.uk/cval/homepage.html
6. Blechl AE, Anderson OD (1996) Expression of a novel high-molecular-weight glutenin subunit gene in transgenic wheat. Nat Biotechnol 14:875-879
7. Boodley JW, Sheldrake R. (1973) Cornell peat-lite mixes for commercial plant growing. Cornell Univ. Inf. Bull. 43, Ithaca, New York
8. Callaway AS, Abranches R, Scroggs J, Allen GC, Thompson WF (2002) High throughput transgene copy number estimation by competitive PCR. Plant Mol Biol Rep 20:265-277
9. Chandrasekharan MB, Kenneth BJ, Timothy HC (2003) Module-specific regulation of the β-phaseolin promoter during embryogenesis. Plant J 33:853-866
10. Chawla HS, Cass LA, Simmonds JA (1999) Expression of anthocyanin pigmentation in wheat tissues transformed with anthocyanin regulatory genes. Curr Sci 76:1365-1370
11. Chen WP, Gu X, Liang GH, Muthukrishnan S, Chen PD, Liu DJ, Gill BS (1998) Introduction and constitutive expression of a rice chitinase gene in bread wheat using biolistic bombardment and the bar gene as a selectable marker. Theor Appl Genet 97:1269-1306
12. Chiang PW, Song WJ, Wu KY, Korenberg JR, Fogel EJ, Van Keuren ML, Lashkari D, Kurnit DM (1996) Use of fluorescent-PCR reaction to detect genomic sequence copy number and transcriptional abundance. Genome Res 6:1013-1026
13. 2DHN, is located on chromosome 6H and has embryo-specific expression. Theor Appl Genet 100:1274-1278
14. Christou P (1996) Transformation technology. Trends Plant Sci 1:423-431
15. de Vetten N, Wolters A-M, Raemakers K, van der Meer I, ter Stege R, Heeres E, Heeres P, Visser R (2003) A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop. Nat Biotechnol 21:439-442
16. Diaz I, Royo J, O'Connor A, Carbonero P (1995) The promoter of the gene Itr1 from barley confers a different tissue specificity in transgenic tobacco. Mol Gen Genet 248:592-598
17. Doshi KM, Eudes F, Laroche A, Gaudet D (2006) Transient embryo-specific expression of anthocyanin in wheat. In Vitro Cell Dev Biol-Plant 42:432-438
18. Eudes F, Acharya S, Laroche A, Selinger LB, Cheng KJ (2003) A novel method to induce direct somatic embryogenesis, secondary embryogenesis and regeneration of fertile green cereal plants. Plant Cell Tissue Organ Cult 73:147-157
19. Gautier MF, Cosson P, Guirao A, Alary R, Joudrier P (2000) Puroindoline genes are highly conserved in diploid ancestor wheat and related species but absent in tetraploid Triticum species. Plant Sci 153:81-91
20. Hansen G, Wright MS (1999) Recent advances in the transformation of plants. Trends Plant Sci 4:226-231
21. Hwang Y-S, Nichol S, Nandi S, Jernstedt JA, Huang N (2001) Aleurone-and embryo-specific expression of the β-glucuronidase gene controlled by the barley Chi26 and Ltp1 promoters in transgenic rice. Plant Cell Rep 20:647-654
22. Ingham DJ, Beer S, Money S, Hansen G (2001) Quantitative real-time PCR assay for determining transgene copy number in transformed plants. Biotechniques 31:132-140
23. Iyer LM, Kumpatla SP, Chandrasekharan MB, Hall TC (2000) Transgene silencing in monocots. Plant Mol Biol 43:323-346
24. Kohli A, Leech M, Vain P, Laurie DA, Christou P (1998) Transgene organization in rice engineered through direct DNA transfer supports a two-phase integration mechanism mediated by the establishment of integration hot spots. Proc Natl Acad Sci U S A 95:7203-7208
25. Li B, Leung N, Caswell K, Chibbar RN (2003) Recovery and characterization of transgenic plants from two spring wheat cultivars with low embryogenesis efficiencies by the bombardment of isolated scutella. In Vitro Cell Dev Biol-Plant 39:12-19
26. Li Z, Hansen JL, Liu Y, Zemetra RS, Berger PH (2004) Using real-time PCR to determine transgene copy number in wheat. Plant Mol Biol Rep 22:179-188
27. ΔΔCt method. Methods 25:402-408
28. Livak KJ, Flood SJ, Marmaro J, Giusti W, Deetz K (1995) Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization. PCR Methods Appl 4:357-362
29. Nehra SH, Chibbar RN, Leung N, Caswell K, Mallard C, Steinhauer L, Baga M, Kartha KK (1994) Self-fertile transgenic wheat plants regenerated from isolated scutellar tissues following microprojectile bombardment with two distinct gene constructs. Plant J 5:285-297
30. Pastori GM, Wilkinson MD, Steele SH, Sparks CA, Jones HD, Parry MAJ (2001) Age-dependent transformation frequency in elite wheat varieties. J Exp Bot 52:857-863
31. Rasco-Gaunt S, Riley A, Cannell M, Barcelo P, Lazzeri PA (2001) Procedures allowing the transformation of a range of European elite wheat (Triticum aestivum L.) varieties via particle bombardment. J Exp Bot 52:865-874
32. Sambrook J, Russel DW (2001) Detection of DNA in agarose gels. In: Molecular Cloning, A laboratory Manual, 3rd edn. New York: Cold Spring Harbor Laboratory Press: 5.14-5.17
33. Schmittgen TD, Zakrajsek BA, Mills AG, Gorn V, Singer MJ, Reed MW (2000) Quantitative reverse transcription-polymerase chain reaction to study mRNA decay, comparison of endpoint and real-time methods. Anal Biochem 285:194-204
34. Song P, Cai CQ, Skokut M, Kosegi BD, Petolino JF (2002) Quantitative real-time PCR as a screening tools for estimating transgene copy number in WHISKERS™ derived transgenic maize. Plant Cell Rep 20:948-954
35. Srivastava V, Anderson OD, Ow DW (1999) Single-copy transgenic wheat generated through the resolution of complex integration patterns. Proc Natl Acad Sci U S A 96:11117-11121
36. Stam M, Mol JNM, Kooter JM (1997) The silence of genes in transgenic plants. Ann Bot 79:3-12
37. Terzi V, Pastori G, Shewry PR, Di Fonzo N, Stanca AM, Faccioli P (2005) Real-time PCR assisted selection of wheat plants transformed with HMW glutenin subunit genes. J Cereal Sci 41:133-136
38. Tranquilli G, Lijavetzky D, Muzzi G, Dubcovsky J (1999) Genetic and physical characterization of grain texture-related loci in diploid wheat. Mol Gen Genet 262:846-850
39. Vasil V, Castillo AM, Fromm MF, Vasil IK (1992) Herbicide resistant fertile transgenic wheat plants obtained by microprojectile bombardment of regenerable embryogenic callus. Bio/technology 10:667-674
40. Vaucheret H, Beclin C, Elmayan T, Feuerbach F, Godon C, Morel JB, Mourrain P, Palauqui JC, Vernhetters S (1998) Transgene-induced gene silencing in plants. Plant J 16:651-659
41. Winer J, Jung CK, Shackel I, Williams PM (1999) Development and validation of real-time quantitative reverse transcription polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro. Anal Biochem 270:41-49