A solubility optimization protocol for two-dimensional gel electrophoresis of cardiac tissue

Methods in Molecular Biology

Volumn 357, Issue , 2007, Pages 59-65

  Stanley, Brian A ^a   Van Eyk, Jennifer E ^a  

^a NONE

Author keywords

2DE; Heart muscle; IEF; Optimization; Solubilization

Indexed keywords

PROTEOME;

ARTICLE; CHEMICAL STRUCTURE; HEART MUSCLE; HUMAN; ISOELECTRIC FOCUSING; METABOLISM; METHODOLOGY; REPRODUCIBILITY; SOLUBILITY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; ISOELECTRIC FOCUSING; MOLECULAR STRUCTURE; MYOCARDIUM; PROTEOME; REPRODUCIBILITY OF RESULTS; SOLUBILITY;

EID: 36549081865     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-214-9:59     Document Type: Article

References (6)

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3. Herbert, B. (1999) Advances in protein solubilization for two-dimensional electrophoresis. Electrophoresis 20, 660-663.
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5. Ames, G. F. and Nikaido, K. (1976) Two-dimensional electrophoresis of membrane proteins. Biochemistry 15, 616-623.
6. Stanley, B. A., Neverova, I., Brown, H. A, and Van Eyk, J. E. (2003) Optimizing protein solubility for two-dimensional analysis of human myocardium. Proteomics 3, 815-820.