Optimizing protein solubility for two-dimensional gel electrophoresis analysis of human myocardium

Proteomics

Volumn 3, Issue 6, 2003, Pages 815-820

  Stanley, Brian A ^a   Neverova, Irina ^a   Brown, Heather A ^a   Van Eyk, Jennifer E ^a  

^a QUEEN S UNIVERSITY   (Canada)

Author keywords

Cardiac; Detergent; Optimization Two dimensional gel electrophoresis

Indexed keywords

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; AMIDOSULFOBETAINE 14; BETAINE DERIVATIVE; DETERGENT; N DECYL N,N' DIMETHYL 3 AMMONIO 1 PROPANESULFONATE; PROTEIN; PROTEOME; SULFONIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

CONFERENCE PAPER; EXTRACTION; GEL ELECTROPHORESIS; HEART LEFT VENTRICLE; HEART MUSCLE; HUMAN; HUMAN TISSUE; ISOELECTRIC FOCUSING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; SOLUBILITY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

BUFFERS; DETERGENTS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HEART VENTRICLES; HUMANS; ISOELECTRIC FOCUSING; MUSCLE PROTEINS; MYOCARDIUM; PROTEOME; SOLUBILITY; TISSUE EXTRACTS;

EID: 0037709388     PISSN: 16159853     EISSN: None     Source Type: Journal    
DOI: 10.1002/pmic.200300388     Document Type: Conference Paper

References (11)

1. Chevallet, M., Santoni, V., Polnas, A., Rouquie, D. et al. Electrophoresis 1998, 19, 1901-1909.
2. Rabilloud, T., Electrophoresis 1996, 17, 813-829.
3. Rabilloud, T., Methods Mol. Biol. 1999, 112, 9-19.
4. Ames, G. F.-L, Nikaido, K., Biochemistry 1976, 15, 616-623.
5. Harder, A., Wildgruber, R., Nawrocki, A., Fey, S. J. et al., Electrophoresis 1999, 20, 826-829.
6. Herbert, B., Electrophoresis 1999, 20, 660-663.
7. Rabilloud, T., Adessi, C., Giraudel, A., Lunardi, J., Electrophoresis 1997, 19, 307-316.
8. Molloy, M. P., Electrophoresis 1998, 19, 837-844.
9. Rabilloud, T., Adessi, C., Giraudel, A., Lunardi, J., Electrophoresis 1997, 18, 307-316.
10. Laemmli, U. K., Nature 1970, 227, 680-685.
11. Schevchenko, A., Wilm, M., Vorm, O., Mann, M., Anal. Chem. 1996, 68, 850-858.