Intein-mediated peptide arrays for epitope mapping and kinase/phosphatase assays

Methods in Molecular Biology

Volumn 381, Issue , 2007, Pages 313-338

  Xu, Ming Qun ^a   Ghosh, Inca ^a   Kochinyan, Samvel ^a   Sun, Luo ^a  

^a NONE

Author keywords

Intein mediated protein ligation; Kinase; Nitrocellulose; Peptide array; Phosphatase; Protein substrate

Indexed keywords

ALANINE; CARRIER PROTEIN; INTEIN; PEPTIDE; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHOSPECIFIC ANTIBODY; PHOSPHOTRANSFERASE;

AMINO ACID SEQUENCE; ANTIBODY SPECIFICITY; ARTICLE; CHEMISTRY; DRUG EFFECT; ENZYME SPECIFICITY; EPITOPE MAPPING; HUMAN; IMMUNOBLOTTING; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN MICROARRAY;

ALANINE; AMINO ACID SEQUENCE; ANTIBODIES, PHOSPHO-SPECIFIC; ANTIBODY SPECIFICITY; CARRIER PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPITOPE MAPPING; HUMANS; IMMUNOBLOTTING; INTEINS; MOLECULAR SEQUENCE DATA; PEPTIDES; PHOSPHOPROTEIN PHOSPHATASES; PHOSPHOTRANSFERASES; PROTEIN ARRAY ANALYSIS; SUBSTRATE SPECIFICITY;

EID: 36549064620     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1385/1-59745-303-X:313     Document Type: Article

References (21)

1. Houseman, B. T., Huh, J. H., Kron, S. J., and Mrksich, M. (2002) Peptide chips for the quantitative evaluation of protein kinase activity. Nat. Biotechnol. 20, 270-274.
2. Lesaicherre, M. L., Uttamchandani, M., Chen, G. Y., and Yao, S. Q. (2002) Antibody-based fluorescence detection of kinase activity on a peptide array. Bioorg. Med. Chem, Lett. 12, 2085-2088.
3. Songyang, Z., Carraway, K. L., Eck, M. J., et al. (1995) Catalytic specificity of protein-tyrosine kinases is critical for selective signaling. Nature 373, 536-539.
4. Falsey, J. R., Renil, M., Park, S., Li, S., and Lam, K. S. (2001) Peptide and small molecule microarray for high throughput cell adhesion and functional assays. Bioconjug. Chem. 12, 346-353.
5. Harlow, E. and Lane, D. (1988) Antibodies, in Immunoassays, (Harlow, E. and Lane, D., eds.). Cold Spring Harbor Laboratory, New York. pp. 553-612.
6. Martens, W., Greiser-Wilke, I., Harder, T. C., et al. (1995) Spot synthesis of over-lapping peptides on paper membrane supports enables the identification of linear monoclonal antibody binding determinants on morbillivirus phosphoproteins. Vet. Microbiol. 44, 289-298.
7. Reineke, U., Ivascu, C., Schlief, M., et al. (2002) Identification of distinct antibody epitopes and mimotopes from a peptide array of 5520 randomly generated sequences. J. Immunol. Methods 267, 37-51.
8. Frank, R. and Overwin, H. (1996) SPOT synthesis. Epitope analysis with arrays of synthetic peptides prepared on cellulose membranes. Methods Mol. Biol. 66, 149-169.
9. Reimer, U., Reineke, U., and Schneider-Mergener, J. (2002) Peptide arrays: from macro to micro. Curr. Opin. Biotechnol. 13, 315-320.
10. Frank, R. (2002) The SPOT-synthesis technique. Synthetic peptide arrays on membrane supports-principles and applications. J. Immunol. Methods 267, 13-26.
11. Reineke, U., Volkmer-Engert, R., and Schneider-Mergener, J. (2001) Applications of peptide anays prepared by the SPOT-technology. Curr. Opin. Biotechnol. 12, 59-64.
12. Sun, L., Rush, J., Ghosh, I., Maunus, J. R., and Xu, M. -Q. (2004) Producing peptide arrays for epitope mapping by intein-mediated protein ligation. Biotechniques 37,430-443.
13. Ghosh, I., Sun, L., Evans, T. C., Jr., and Xu, M. -Q. (2004) An improved method for utilization of peptide substrates for antibody characterization and enzymatic assays. J. Immunol. Methods 293, 85-95.
14. Xu, J., Sun, L., Ghosh, I., and Xu, M. Q. (2004) Western blot analysis of Src kinase assays using peptide substrates ligated to a carrier protein. Biotechniques 36, 976-981.
15. Evans, T. C., Jr., Benner, J., and Xu, M. Q. (1998) Semisynthesis of cytotoxic proteins using a modified protein splicing element. Protein Sci. 7, 2256-2264.
16. Evans, T. C., Jr. and Xu, M. Q. (1999) Intein-mediated protein ligation: harnessing nature's escape artists. Biopolymers 51, 333-342.
17. Muir, T. W., Sondhi, D., and Cole, P. A (1998) Expressed protein ligation: a general method for protein engineering. Proc. Natl. Acad. Sci. USA 95, 6705-6710.
18. Chong, S., Mersha, F. B., Comb, D. G., et al. (1997) Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192, 271-281.
19. Lesaicherre, M. L., Lue, R. Y., Chen, G. Y., Zhu, Q., and Yao, S. Q. (2002) Inteinmediated biotinylation of proteins and its application in a protein microarray. J. Am. Chem. Soc. 124, 8768-8769.
20. Tan, L. P., Lue, R. Y., Chen, G. Y., and Yao, S. Q. (2004) Improving the inteinmediated, site-specific protein biotinylation strategies both in vitro and in vivo. Bioorg. Med. Chem. Lett. 14, 6067-6070.
21. Sun, L., Ghosh, I., and Xu, M. Q. (2003) Generation of an affinity column for antibody purification by intein-mediated protein ligation. J. Immunol. Methods 282, 45-52.