-
1
-
-
1542285371
-
Design of bacterial vector systems for the production of recombinant proteins in Escherichia coli
-
Mergulhão, F. J. M., Monteiro, G. A., Cabrai, J. M. S., and Taipa, M. A. (2004) Design of bacterial vector systems for the production of recombinant proteins in Escherichia coli. J. Microb. Biotechnol. 14, 1-14.
-
(2004)
J. Microb. Biotechnol
, vol.14
, pp. 1-14
-
-
Mergulhão, F.J.M.1
Monteiro, G.A.2
Cabrai, J.M.S.3
Taipa, M.A.4
-
2
-
-
8344256552
-
Recombinant protein folding and misfolding in Escherichia coli
-
Baneyx, F. and Mujacic, M. (2004) Recombinant protein folding and misfolding in Escherichia coli. Nat. Biotechnol. 22, 1399-1408.
-
(2004)
Nat. Biotechnol
, vol.22
, pp. 1399-1408
-
-
Baneyx, F.1
Mujacic, M.2
-
3
-
-
14844283810
-
Recombinant protein secretion in Escherichia coli
-
Mergulhão, F. J. M. and Monteiro, G. A. (2005) Recombinant protein secretion in Escherichia coli. Biotechnol. Adv. 23, 177-202.
-
(2005)
Biotechnol. Adv
, vol.23
, pp. 177-202
-
-
Mergulhão, F.J.M.1
Monteiro, G.A.2
-
4
-
-
0030271490
-
Secretion of recombinant proteins by Gram-negative bacteria
-
Sandkvist, M., and Bagdasarian, M. (1996) Secretion of recombinant proteins by Gram-negative bacteria. Curr. Opin. Biotechnol. 7, 505-511.
-
(1996)
Curr. Opin. Biotechnol
, vol.7
, pp. 505-511
-
-
Sandkvist, M.1
Bagdasarian, M.2
-
5
-
-
0037294121
-
Cell and process design for targeting of recombinant protein into the culture medium of Escherichia coli
-
Shokri, A., Sandén, A. M., and Larsson, G. (2003) Cell and process design for targeting of recombinant protein into the culture medium of Escherichia coli. Appl. Microbiol. Biotechnol. 60, 654-664.
-
(2003)
Appl. Microbiol. Biotechnol
, vol.60
, pp. 654-664
-
-
Shokri, A.1
Sandén, A.M.2
Larsson, G.3
-
6
-
-
0442292087
-
Minimizing inclusion body formation during recombinant protein production in Escherichia coli at bench and pilot plant scale
-
Hoffmann, F., van den Heuvel, J., Zidek, N., and Rinas, U. (2004) Minimizing inclusion body formation during recombinant protein production in Escherichia coli at bench and pilot plant scale. Enzyme Microb. Tech. 34, 235-241.
-
(2004)
Enzyme Microb. Tech
, vol.34
, pp. 235-241
-
-
Hoffmann, F.1
van den Heuvel, J.2
Zidek, N.3
Rinas, U.4
-
7
-
-
0037071906
-
Construction and deconstruction of bacterial inclusion bodies
-
Carrio, M. M., and Villaverde, A. (2002) Construction and deconstruction of bacterial inclusion bodies. J. Biotechnol. 96, 3-12.
-
(2002)
J. Biotechnol
, vol.96
, pp. 3-12
-
-
Carrio, M.M.1
Villaverde, A.2
-
8
-
-
0029828233
-
Strategies for achieving high-level expression of genes in Escherichia coli
-
Makrides, S. C. (1996) Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 60, 512-538.
-
(1996)
Microbiol. Rev
, vol.60
, pp. 512-538
-
-
Makrides, S.C.1
-
9
-
-
0033757725
-
Recombinant human proinsulin: A new approach in gene assembly and protein expression
-
Mergulhão, F., Monteiro, G., Kelly, A., Taipa, M., and Cabrai, J. (2000) Recombinant human proinsulin: A new approach in gene assembly and protein expression. J. Microbiol. Biotechnol. 10, 690-693.
-
(2000)
J. Microbiol. Biotechnol
, vol.10
, pp. 690-693
-
-
Mergulhão, F.1
Monteiro, G.2
Kelly, A.3
Taipa, M.4
Cabrai, J.5
-
10
-
-
3042672710
-
Removal of N-terminal methionine from recombinant proteins by engineered E. coli methionine aminopeptidase
-
Liao, Y. D., Jeng, J. C., Wang, C. F., Wang, S. C., and Chang, S.T. (2004) Removal of N-terminal methionine from recombinant proteins by engineered E. coli methionine aminopeptidase. Protein Sci. 13, 1802-1810.
-
(2004)
Protein Sci
, vol.13
, pp. 1802-1810
-
-
Liao, Y.D.1
Jeng, J.C.2
Wang, C.F.3
Wang, S.C.4
Chang, S.T.5
-
11
-
-
0030444320
-
Proteases and their targets in Escherichia coli
-
Gottesman, S. (1996) Proteases and their targets in Escherichia coli. Annu. Rev. Genet. 30, 465-506.
-
(1996)
Annu. Rev. Genet
, vol.30
, pp. 465-506
-
-
Gottesman, S.1
-
12
-
-
25844514728
-
Preparative expression of secreted proteins in bacteria: Status report and future prospects
-
Georgiou, G. and Segatori, L. (2005) Preparative expression of secreted proteins in bacteria: status report and future prospects. Curr. Opin. Biotechnol. 16, 538-545.
-
(2005)
Curr. Opin. Biotechnol
, vol.16
, pp. 538-545
-
-
Georgiou, G.1
Segatori, L.2
-
13
-
-
0023664821
-
Expression of human insulinlike growth factor I in bacteria: Use of optimized gene fusion vectors to facilitate protein purification
-
Moks, T., Abrahmsen, L., Holmgren, E., et al. (1987) Expression of human insulinlike growth factor I in bacteria: use of optimized gene fusion vectors to facilitate protein purification. Biochemistry 26, 5239-5244.
-
(1987)
Biochemistry
, vol.26
, pp. 5239-5244
-
-
Moks, T.1
Abrahmsen, L.2
Holmgren, E.3
-
14
-
-
0031045390
-
The use of gene fusions to protein A and protein G in immunology and biotechnology
-
Stahl, S. and Nygren, P.A. (1997) The use of gene fusions to protein A and protein G in immunology and biotechnology. Pathol. Biol. (Paris) 45, 66-76.
-
(1997)
Pathol. Biol. (Paris)
, vol.45
, pp. 66-76
-
-
Stahl, S.1
Nygren, P.A.2
-
15
-
-
0002737940
-
Affinity fusions, gene expression
-
Flickinger, M, and Drew, S, eds, John Wiley & Sons, New York, pp
-
Stahl, S., Nilsson, J., Hober, S., Uhlen, M., and Nygren, P. (1999) Affinity fusions, gene expression. In Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation (Flickinger, M., and Drew, S., eds.), John Wiley & Sons, New York, pp. 49-63.
-
(1999)
Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis and Bioseparation
, pp. 49-63
-
-
Stahl, S.1
Nilsson, J.2
Hober, S.3
Uhlen, M.4
Nygren, P.5
-
16
-
-
0033428016
-
Troubleshooting in gene splicing by overlap extension: A step-wise method
-
Mergulhão, F. J., Kelly, A. G., Monteiro, G. A., Taipa, M. A., and Cabral, J. M. (1999) Troubleshooting in gene splicing by overlap extension: a step-wise method. Mol. Biotechnol. 12, 285-287.
-
(1999)
Mol. Biotechnol
, vol.12
, pp. 285-287
-
-
Mergulhão, F.J.1
Kelly, A.G.2
Monteiro, G.A.3
Taipa, M.A.4
Cabral, J.M.5
-
17
-
-
0042591085
-
Evaluation of inducible promoters on the secretion of a ZZ-Proinsulin fusion protein
-
Mergulhão, F. J. M., Monteiro, G. A., Larsson, G., et al. (2003) Evaluation of inducible promoters on the secretion of a ZZ-Proinsulin fusion protein. Biotechnol. Appl. Biochem. 38, 87-93.
-
(2003)
Biotechnol. Appl. Biochem
, vol.38
, pp. 87-93
-
-
Mergulhão, F.J.M.1
Monteiro, G.A.2
Larsson, G.3
-
18
-
-
34548502969
-
Analysis of factors affecting the periplasmic production of recombinant proteins in Escherichia coli
-
Mergulhão, F. J. M., and Monteiro, G. A. (2007) Analysis of factors affecting the periplasmic production of recombinant proteins in Escherichia coli. J. Microbiol. Biotechnol. 17.
-
(2007)
J. Microbiol. Biotechnol
, vol.17
-
-
Mergulhão, F.J.M.1
Monteiro, G.A.2
-
19
-
-
0023056981
-
Secretion of heterologous gene products to the culture medium of Escherichia coli
-
Abrahmsen, L., Moks, T., Nilsson, B., and Uhlen, M. (1986) Secretion of heterologous gene products to the culture medium of Escherichia coli. Nucleic Acids Res. 14, 7487-7500.
-
(1986)
Nucleic Acids Res
, vol.14
, pp. 7487-7500
-
-
Abrahmsen, L.1
Moks, T.2
Nilsson, B.3
Uhlen, M.4
-
20
-
-
1842425886
-
Evaluation of bottlenecks in proinsulin secretion by Escherichia coli
-
Mergulhão, F. J., Taipa, M. A., Cabral, J. M., and Monteiro, G. A. (2004) Evaluation of bottlenecks in proinsulin secretion by Escherichia coli. J. Biotechnol. 109, 31-43.
-
(2004)
J. Biotechnol
, vol.109
, pp. 31-43
-
-
Mergulhão, F.J.1
Taipa, M.A.2
Cabral, J.M.3
Monteiro, G.A.4
-
21
-
-
0024835779
-
Thiol-directed immobilization of recombinant IgG-binding receptors
-
Ljungquist, C., Jansson, B., Moks, T., and Uhlen, M. (1989) Thiol-directed immobilization of recombinant IgG-binding receptors. Eur. J. Biochem. 186, 557-561.
-
(1989)
Eur. J. Biochem
, vol.186
, pp. 557-561
-
-
Ljungquist, C.1
Jansson, B.2
Moks, T.3
Uhlen, M.4
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