TIM crystals grown by capillary counterdiffusion: Statistical evidence of quality improvement in microgravity

Crystal Growth and Design

Volumn 7, Issue 11, 2007, Pages 2161-2166

  Evrard, Christine ^a,c   Maes, Dominique ^b   Zegers, Ingrid ^b,d   Declercq, Jean Paul ^c   Vanhee, Celine ^b   Martial, Joseph ^a   Wyns, Lode ^b   Van De Weerdt, Cécile ^b  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

^c UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

^d JOINT RESEARCH CENTRE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 36549039601     PISSN: 15287483     EISSN: None     Source Type: Journal    
DOI: 10.1021/cg700687t     Document Type: Conference Paper

References (26)

1. Beaucamp, N.; Hofmann, A.; Kellerer, B.; Jaenicke, R. Dissection of the gene of the bifunctional PGK-TIM fusion protein from the hyperthermophilic bacterium Thermotoga maritima: design and characterization of the separate triosephosphate isomerase. Protein Sci. 1997, 6, 2159-2165.
2. Boggon, T. J.; Chayen, N. E.; Snell, E. H.; Dong, J.; Lautenschlager, P.; Potthast, L.; Siddons, D. P.; Stojanoff, V.; Gordon, E.; Thompson, A. W.; Zagalsky, P. F.; Bi, R. C.; Helliwell, J. R. Protein crystal movements and fluid flows during microgravity growth. Philos. Trans. R. Soc. London, Ser. A 1998, 356, 1045-1061.
3. Chayen, N. E.; Saridakis, E. Protein crystallization for genomics: towards high-throughput optimization techniques. Acta Crystallogr., Sect. D 2002, 58, 921-927.
4. Chayen, N. E. Turning protein crystallisation from an art into a science. Curr. Opin. Struct. Biol. 2004, 14, 577-583.
5. Garcia-Ruiz, J. M.; Moreno, A.; Viedma, C.; Coll, M. Crystal quality of lysozyme single crystals grown by the gel acupuncture method. Mater. Res. Bull. 1993, 28, 541-546.
6. Garcia-Ruiz, J. M.; Moreno, A. Investigations on protein crystal growth by the gel acupuncture method. Acta Crystallogr., Sect. D 1994, 50, 484-490.
7. García-Ruiz, J. M.; Otálora, F. Crystal growth studies in microgravity with the APCF. II. Image analysis studies. J. Cryst. Growth 1997, 182, 155-167.
8. Garcia-Ruiz, J. M.; Gavira, J. A.; Otalora, F.; Guasch, A.; Coll, M. Reinforced protein crystals. Mater. Res. Bull. 1998, 33, 1593-1598.
9. Garcia-Ruiz, J. M.; Otalora, F.; Novella, M. L.; Gavira, J. A.; Sauter, C.; Vidal, O. A supersaturation wave of protein crystallization. J. Cryst. Growth 2001, 232, 149-155.
10. García-Ruiz, J. M.; Drenth, J.; Ries-Kautt, M.; Tardieu, A. A World without Gravity: Research in Space for Health and Industrial Processes; Seibert, G., Ed.; European Space Agency: Paris, 2001; Vol. 1251, pp 150-171.
11. Garcia-Ruiz, J. M.; Gonzalez-Ramirez, L. A.; Gavira, J. A.; Otalora, F. Granada Crystallisation Box: a new device for protein crystallisation by counter-diffusion techniques. Acta Crystallogr., Sect. D 2002, 58, 1638-1642.
12. Gavira, J. A.; Garcia-Ruiz, J. M. Agarose as crystallisation media for proteins II: trapping of gel fibres into the crystals. Acta Crystallogr., Sect. D 2002, 58, 1653-1656.
13. Judge, R. A.; Snell, E. H.; van der Woerd, M. J. Extracting trends from two decades of microgravity macromolecular crystallization history. Acta Crystallogr., Sect. D 2005, 61, 763-771.
14. Kabsch, W. J. Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants. J. Appl. Crystallogr. 1993, 26, 795-800.
15. Kundrot, C. E.; Judge, R. A.; Pusey, M. L.; Snell, E. H. Microgravity and macromolecular crystallography. Cryst. Growth Des. 2001, 1, 87-99.
16. Maes, D.; Zeelen, J. P.; Thanki, N.; Beaucamp, N.; Alvarez, M.; Thi, M. H.; Backmann, J.; Martial, J. A.; Wyns, L.; Jaenicke, R.; Wierenga, R. K. The crystal structure of triosephosphate isomerase (TIM) from Thermotoga maritima: a comparative thermostability structural analysis of ten different TIM structures. Proteins 1999, 37, 441-453.
17. Matsumoto, S.; Yoda, S. J. Numerical study of diffusion coefficient measurements with sinusoidal varying accelerations. J. Appl. Phys. 1999, 85, 8131-8136.
18. Mcpherson, A. Macromolecular crystal growth in microgravity. Crystallogr. Rev. 1999, 6, 157-305.
19. Otálora, F.; Garcia-Ruiz, J. M. Crystal growth studies in microgravity with the APCF I. Computer simulation of transport dynamics. J. Cryst. Growth 1997, 182, 141-154.
20. Otwinowski, Z.; Minor, W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 1997, 276, 307-326.
21. Pusey, M.; Witherow, W. K.; Nauman, R. Preliminary investigations into solutal flow about growing tetragonal lysozyme crystals. J. Cryst. Growth 1988, 90, 105-111.
22. Snell, E. H.; Boggon, T. J.; Helliwell, J. R.; Moskowitz, M. E.; Nadarajah, A. CCD video observation of microgravity crystallization of lysozyme and correlation with accelerometer data. Acta Crystallogr., Sect. D 1997, 53, 747-755.
23. Snell, E. H.; Helliwell, J. R. Macromolecular crystallization in microgravity. Rep. Prog. Phys. 2005, 68, 799-853.
24. Vergara, A.; Lorber, B.; Zagari, A.; Giege, R. Physical aspects of protein crystal growth investigated with the Advanced Protein Crystallization Facility in reduced-gravity environments. Acta Crystallogr, Sect. D 2003, 59, 2-15.
25. Zegers, I.; Carotenuto, L.; Evrard, C.; Garcia-Ruiz, J. M.; De Gieter, P.; Gonzalez-Ramirez, L. A.; Istasse, E.; Legros, J.; Martial, J. A.; Minetti, C.; Otalora, F.; Queeckers, P.; Schockaert, C.; Van De Weerdt, C.; Willaert, R.; Wyns, L.; Yourrassowsky, C.; Dubois, F. Counter-diffusion protein crystallisation in microgravity and its observation with PromlSS (Protein Microscope for the International Space Station). Microgravity Sci. Technol. 2006, 23, 165-169.
26. Zegers, I.; Van De Weerdt, C.; Evrard, C.; Vanhee, C.; Sleutel, M.; Willaert, R.; Declercq, J. P.; Maes, D.; Otalora, F.; Garcia-Ruiz, J. M. 2007, unpublished work.