Characterization of dominant cultivable lactobacilli and their antibiotic resistance profiles from faecal samples of weaning piglets

Journal of Applied Microbiology

Volumn 103, Issue 6, 2007, Pages 2496-2503

  Korhonen, J M ^a   Sclivagnotis, Y ^a   Wright, A Von ^a  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

Author keywords

Antibiotics; Lactic acid bacteria; Lactobacillus reuteri; Minimal inhibitory concentration; Pigs; Plate dilution method; Randomly amplified polymorphic DNA

Indexed keywords

BACILLI; DNA; LACTIC ACID; MAMMALS; POPULATION DISTRIBUTION;

DILUTION METHOD; FAECAL SAMPLES; L.SALIVARIUS; LACTIC ACID BACTERIA; LACTOBACILLUS REUTERI; LACTOBACILLUS SPECIES; MINIMAL INHIBITORY CONCENTRATION; PIG; PLATE DILUTION METHOD; RANDOMLY AMPLIFIED POLYMORPHIC DNA;

ANTIBIOTICS;

AMPICILLIN; ANTIBIOTIC AGENT; CHLORAMPHENICOL; DNA 16S; GENTAMICIN;

ANTIBIOTIC RESISTANCE; BACTERIUM; DNA; FECES; MORPHOLOGY; PHENOTYPE;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM EXAMINATION; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CONTROLLED STUDY; DNA DETERMINATION; DNA FINGERPRINTING; DNA ISOLATION; FECES; GENETIC SUSCEPTIBILITY; LACTIC ACID BACTERIUM; LACTOBACILLUS; LACTOBACILLUS GALLINARUM; LACTOBACILLUS JOHNSONII; LACTOBACILLUS MUCOSAE; LACTOBACILLUS REUTERI; LACTOBACILLUS SALIVARIUS; LACTOBACILLUS VAGINALIS; MORPHOLOGY; NONHUMAN; PHENOTYPE; PIGLET; RANDOM AMPLIFIED POLYMORPHIC DNA; SPECIES IDENTIFICATION; STRAIN IDENTIFICATION; WEANING;

ANIMALIA; LACTOBACILLUS; LACTOBACILLUS ACIDOPHILUS; LACTOBACILLUS AGILIS; LACTOBACILLUS GALLINARUM; LACTOBACILLUS JOHNSONII; LACTOBACILLUS MUCOSAE; LACTOBACILLUS REUTERI; LACTOBACILLUS SALIVARIUS; LACTOBACILLUS VAGINALIS; SUIDAE;

EID: 36549019564     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2007.03483.x     Document Type: Article

References (31)

1. Abe, F., Ishibashi, N. Shimamura, S. (1995) Effect of administration of bifidobacteria and lactic acid bacteria to newborn calves and piglets. J Dairy Sci 78, 2838 2846.
2. Al Jassim, R.A.M. (2003) Lactobacillus ruminis is a predominant lactic acid producing bacterium in the caecum and rectum of the pig. Lett Appl Microbiol 37, 213 217.
3. Allison, M.J., Robinson, I.M., Bucklin, J.A. Booth, G.D. (1979) Comparison of bacterial populations of the pig cecum and colon based upon enumeration with specific energy sources. Appl Environ Microbiol 37, 1142 1151.
4. Axelsson, L. Lindgren, S. (1987) Characterization and DNA homology of Lactobacillus strain isolated from pig intestine. J Appl Bacteriol 62, 433 440.
5. Baele, M., Vaneechoutte, M., Verhelst, R., Vancanneyt, M., Devriese, L.A. Haesebrouck, F. (2002) Identification of Lactobacillus species using tDNA-PCR. J Microbiol Methods 50, 263 271.
6. Chagnaud, P., Machinis, K., Coutte, L.A., Marecat, A. Mercenier, A. (2001) Rapid PCR-based procedure to identify lactic acid bacteria: application to six common Lactobacillus species. J Microbiol Methods 44, 139 148.
7. Danielsen, M. Wind, A. (2003) Susceptibility of Lactobacillus spp. to antimicrobial agents. Int J Food Microbiol 82, 1 11.
8. De Angelis, M., Corsetti, A., Tosti, N., Rossi, J., Corbo, M.R. Gobbetti, M. (2001) Characterization of non-starter lactic acid bacteria from Italian ewe cheeses based on phenotypic, genotypic, and cell wall analysis Appl Environ Microbiol 67, 2011 2020.
9. Du Toit, M., Dicks, L.M.T. Holzapfel, W.H. (2001) Taxonomy of obligately homofermentative and facultatively heterofermentative lactobacilli in pig faeces. Lett Appl Microbiol 32, 199 204.
10. FEEDAP 2005) Opinion of the scientific panel on additives and products or substances used in animal feed on the updating of the criteria used in the assessment of bacteria for resistance to antibiotics of human or veterinary importance. EFSA J 223, 1 12.
11. Fitzsimons, N.A., Cogan, T.M., Condon, S. Beresford, T. (1999) Phenotypic and genotypic characterization of non-starter lactic acid bacteria in mature cheddar cheese. Appl Environ Microbiol 65, 3418 3426.
12. Hayford, A.E., Petersen, A., Vogensen, F.K. Jakobsen, M. (1999) Use of conserved randomly amplified polymorphic DNA (RAPD) fragments and RAPD pattern for characterization of Lactobacillus fermentum in Ghanaian fermented maize dough. Appl Environ Microbiol 65, 3213 3221.
13. Heilig, H.G.H.J., Zoetendal, E.G., Vaughan, E.E., Marteau, P., Akkermans, A.D.L. de Vos, W.M. (2002) Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA. Appl Environ Microbiol 68, 114 123.
14. Henriksson, A., André, L. Conway, P.L. (1995) Distribution of lactobacilli in the porcine gastrointestinal tract. FEMS Microbiol Ecol 16, 55 60.
15. Johansson, M.-L., Quednau, M., Ahrne, S. Molin, G. (1995) Randomly amplified polymorphic DNA (RAPD) for rapid typing of Lactobacillus plantarum strains. Lett Appl Microbiol 21, 155 159.
16. AL. In Bergey's Manual of Systematic Bacteriology, Vol. 2 ed. Sneath, P.H.A., Mair, N.S., Sharpe, M.E. Holt, J.G. pp. 1209 1234. USA : Williams & Wilkins, Baltimore.
17. Klare, I., Konstabel, C., Müller-Bertling, S., Reissbrodt, R., Huys, G., Vancanneyt, M., Swings, J., Goossens, H. et al. (2005) Evaluation of new broth media for microdilution antibiotic susceptibility testing of lactobacilli, pediococci, lactococci, and bifidobacteria. Appl Environ Microbiol 71, 8982 8986.
18. Leser, T.D., Amenuvor, J.Z., Jensen, T.K., Lindecrona, R.H., Boye, M. Møller, K. (2002) Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited. Appl Environ Microbiol 68, 673 690.
19. Luchansky, J.B., Tennant, M.C. Klaenhammer, T.R. (1991) Molecular cloning and DNA polymorphisms in Lactobacillus acidophilus and Lactobacillus gasseri. J Dairy Sci 74, 3293 3302.
20. McCartney, A.L., Wenzhi, W. Tannock, G.W. (1996) Molecular analysis of the composition of the bifidobacterial and Lactobacillus microflora of humans. Appl Environ Microbiol 62, 4608 4613.
21. Moore, W.E.C., Moore, L.V.H., Cato, E.P., Wilkins, T.D. Kornegay, E.T. (1987) Effect of high-fiber and high-oil diets on the fecal flora of swine. Appl Environ Microbiol 53, 1638 1644.
22. Naito, S., Hayashidani, H., Kaneko, K., Ogawa, M. Benno, Y. (1995) Development of intestinal lactobacilli in normal piglets. J Appl Bact 79, 230 236.
23. Pedersen, K. Tannock, G.W. (1989) Colonization of the porcine gastrointestinal tract by lactobacilli. Appl Environ Microbiol 55, 279 283.
24. Pryde, S.E., Richardson, A.J., Stewart, C.S. Flint, H.J. (1999) Molecular analysis of the microbial diversity present in the colonic wall, colonic lumen and cecal lumen of a pig. Appl Environ Microbiol 65, 5372 5377.
25. Salanitro, J.P., Blake, I.G. Muirhead, P.A. (1977) Isolation and identification of fecal bacteria from adult swine. Appl Environ Microbiol 33, 79 84.
26. Simpson, J.M., McCracken, V.J., Gaskins, H.R. Mackie, R.I. (2000) Denaturing gradient gel electrophoresis analysis of 16S ribosomal DNA amplicons to monitor changes in fecal bacterial populations of weaning pigs after introduction of Lactobacillus reuteri strain MM53. Appl Environ Microbiol 66, 4705 4714.
27. Tajima, K., Aminov, R.I., Nagamine, T., Ogata, K., Nakamura, M., Matsui, H. Benno, Y. (1999) Rumen bacterial diversity as determined by sequence analysis of 16S rDNA libraries. FEMS Microbiol Lett 29, 159 169.
28. Tannock, G.W. (1992) The lactic microflora of pigs, mice and rats. In The Lactic Acid Bacteria, Vol. 1 ed. Wood, B.J.B. pp. 21 48 London, U.K : Elsevier Applied Science.
29. Tannock, G.W., Fuller, R. Pedersen, K. (1990) Lactobacillus succession in the piglet digestive tract demonstrated by plasmid profiling. Appl Environ Microbiol 56, 1310 1316.
30. Tilsala-Timisjärvi, A. Alatossava, T. (1997) Development of oligonucleotide primers from the 16S-23S rRNA intergenic sequences for identifying different dairy and probiotic lactic acid bacteria by PCR. Int J Food Microbiol 35, 49 56.
31. Von Aulock, M.H. Holzapfel, W.H. (1987) Frequency and occurrence of Lactobacillus acidophilus in the gut of the pig, as indicated by its presence in the faeces. J Vet Res 54, 581 583.