Mechanisms of [3H]glycine release from mouse spinal cord synaptosomes selectively labeled through GLYT2 transporters

Journal of Neurochemistry

Volumn 103, Issue 6, 2007, Pages 2439-2448

  Luccini, Elisa ^a   Raiteri, Luca ^a  

^a UNIVERSITY OF GENOA   (Italy)

Author keywords

Carrier mediated release; Exocytosis; Glycine release; Glycine transporters; Mouse spinal cord synaptosomes

Indexed keywords

3 ([1,1' BIPHENYL] 4 YLOXY) 3 (4 FLUOROPHENYL)PROPYL N METHYLGLYCINE; 4 AMINOPYRIDINE; 4 BENZYLOXY 3,5 DIMETHOXY N [1 (DIMETHYLAMINOCICLOPHENYL) METHYL] BENZAMIDE; 7 CHLORO 5 (2 CHLOROPHENYL) 1,5 DIHYDRO 4,1 BENZOTHIAZEPIN 2(3H) ONE; BAFILOMYCIN; GLYCINE; GLYCINE H 3; GLYCINE RECEPTOR ANTAGONIST; GLYCINE TRANSPORTER 2; O [(2 BENZYLOXYPHENYL 3 FLUROPHENYL)METHYL] LEVO SERINE; UNCLASSIFIED DRUG; VERATRIDINE;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; EVOKED RESPONSE; EXOCYTOSIS; IMMUNOCYTOCHEMISTRY; MOUSE; NERVE ENDING; NEUROTRANSMITTER RELEASE; NONHUMAN; PRIORITY JOURNAL; SPINAL CORD; SYNAPTOSOME;

ANIMALS; CALCIUM; CALCIUM SIGNALING; EXOCYTOSIS; GLYCINE; GLYCINE PLASMA MEMBRANE TRANSPORT PROTEINS; MICE; MITOCHONDRIA; NEURAL INHIBITION; POTASSIUM; PRESYNAPTIC TERMINALS; SODIUM-CALCIUM EXCHANGER; SPINAL CORD; SYNAPTIC TRANSMISSION; SYNAPTOSOMES; TRITIUM; VERATRIDINE;

EID: 36448954300     PISSN: 00223042     EISSN: 14714159     Source Type: Journal    
DOI: 10.1111/j.1471-4159.2007.04967.x     Document Type: Article

References (53)

1. 2+ exchanger: from molecular biology to therapeutic perspectives. Pharmacol. Rev. 56, 633 654.
2. Aragón C. López-Corcuera B. (2005) Glycine transporters: crucial roles of pharmacological interest revealed by gene deletion. Trends Pharmacol. Sci. 26, 283 286.
3. Atkinson B. N., Bell S. C., De Vivo M. et al. (2001) ALX 5407: a potent, selective inhibitor of the hGlyT1 glycine transporter. Mol. Pharmacol. 60, 1414 1420.
4. Bakker M. J., van Dijk J. G., van den Maagdenberg A. M. Tijssen M. A. (2006) Startle syndromes. Lancet Neurol. 5, 513 524.
5. Barker G. A. Ellory J. C. (1990) The identification of neutral amino acid transport system. Exp. Physiol. 75, 3 26.
6. 2+ homeostasis in cultured rat dorsal root ganglion neurons. Eur. J. Pharmacol. 340, 295 300.
7. Bernath S. (1992) Calcium-independent release of amino acid neurotransmitters: fact or artifact? Prog. Neurobiol. 38, 57 91.
8. Betz H. (1992) Structure and function of inhibitory glycine receptors. Q. Rev. Biophys. 25, 381 394.
9. Blaustein M. P. (1975) Effects of potassium, veratridine and scorpion venom on calcium accumulation and transmitter release by nerve terminals in vitro. J. Physiol. Lond. 247, 617 655.
10. Bradford M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248 254.
11. Caulfield W. L., Collie I. T., Dickins R. S. et al. (2001) The first potent and selective inhibitors of the glycine transporter type 2. J. Med. Chem. 44, 2679 2682.
12. Chaudhry F. A., Reimer R. J., Bellocchio E. E., Danbolt N. C., Osen K. K., Edwards R. H. Storm-Mathisen J. (1998) The vesicular GABA transporter VGAT, localizes to synaptic vesicles in sets of glycinergic as well as GABAergic neurons. J. Neurosci. 18, 9733 9750.
13. 2+ exchanger in the regulation of oxidative phosphorylation in isolated heart mitochondria. J. Biol. Chem. 268, 938 947.
14. Cubelos N., Gimenéz C. Zafra F. (2005) Localization of the GLYT1 glycine transporter at glutamatergic synapses in the rat brain. Cereb. Cortex 15, 448 459.
15. Dunkley P. R., Heath J. W., Harrison S. M., Jarvie P. E., Glenfield P. J. Rostas J. A. (1988) A rapid Percoll gradient procedure for isolation of synaptosomes directly from an S1 fraction: homogeneity and morphology of subcellular fractions. Brain Res. 441, 59 71.
16. Eulenberg V., Armsen W., Betz H. Gomeza J. (2005) Glycine transporters: essential regulators of neurotransmission. Trends Biochem. Sci. 30, 325 333.
17. +-ATPase of mammalian synaptic vesicles. J. Neurochem. 55, 1663 1670.
18. Foran P., Lawrence G. W., Shone C. C., Foster K. A. Dolly J. O. (1996) Botulinum neurotoxin C1 cleaves both syntaxin and SNAP-25 in intact and permeabilized chromaffin cells: correlation with its blockade of catecholamine release. Biochemistry 25, 2630 2636.
19. Gadea A. Lopez-Colome A. M. (2001) Glial transporters for glutamate, glycine and GABA. III. Glycine transporters. J. Neurosci. Res. 64, 218 222.
20. Galli A., Mori F., Bargellini M. Coppini L. (1993) Sodium-dependent release of exogenous glycine from preloaded rat hippocampal synaptosomes. J. Neural Transm. 93, 167 179.
21. Geerlings A., Núñez E., López-Corcuera B. Aragón C. (2001) Calcium- and syntaxin 1-mediated trafficking of the neuronal glycine transporter GLYT2. J. Biol. Chem. 276, 17584 17590.
22. Gomeza J., Ohno K., Hülsmann S., Armsen W., Eulenburg V., Richter D. W., Laube B. Betz H. (2003) Deletion of the mouse glycine transporter 2 results in a hyperekplexia phenotype and postnatal lethality. Neuron 40, 797 806.
23. Gunter T. E., Gunter T. K., Sheu S.-S. Gavin C. E. (1994) Mitochondrial calcium transport: physiological and pathological relevance. Am. J. Physiol. 267, C313 C339.
24. Herdon H. J., Godfrey F. M., Brown A. M., Coulton S., Evans J. R. Cairns W. J. (2001) Pharmacological assessment of the role of the glycine transporter GlyT-1 in mediating high-affinity glycine uptake by rat cerebral cortex and cerebellum synaptosomes. Neuropharmacology 41, 88 96.
25. Johnson J. W. Ascher P. (1987) Glycine potentiates the NMDA response in cultured mouse brain neurons. Nature 325, 529 531.
26. Jonas P., Bischofberger J. Sandkühler J. (1998) Corelease of two fast neurotransmitters at a central synapse. Science 281, 419 424.
27. Laube B., Maksay G., Schemm R. Betz H. (2002) Modulation of glycine receptor function: a novel approach for therapeutic intervention at inhibitory synapses? Trends Pharmacol. Sci. 23, 519 527.
28. Legendre P. (2001) The glycinergic inhibitory synapse. Cell. Mol. Life Sci. 58, 760 793.
29. Levi G. Raiteri M. (1974) Exchange of neurotransmitter amino acid at nerve endings can simulate high affinity uptake. Nature 250, 735 737.
30. Levi G. Raiteri M. (1993) Carrier-mediated release of neurotransmitters. Trends Neurosci. 16, 415 419.
31. Levi G., Bernardi G., Cherubini E., Gallo V., Marciani M. G. Stanzione P. (1982) Evidence in favor of a neurotransmitter role of glycine in the rat cerebral cortex. Brain Res. 236, 121 131.
32. López-Corcuera B., Geerlings A. Aragón C. (2001) Glycine neurotransmitter transporters: an update. Mol. Membr. Biol. 18, 13 20.
33. Lynch J. W. Callister R. A. (2006) Glycine receptors: a new therapeutic target in pain pathways. Curr. Opin. Invest. Drugs 7, 48 53.
34. Mahendrasingam S., Wallam C. A. Hackney C. M. (2000) An immunogold investigation of the relationship between the amino acids GABA and glycine and their transporters in terminals in the guinea-pig anteroventral cochlear nucleus. Brain Res. 887, 47 481.
35. Nakamura Y., Iga K., Shibata T., Shudo M. Kataoka K. (1993) Glial plasmalemmal vesicles: a subcellular fraction from rat hippocampal homogenate distinct from synaptosomes. Glia 9, 48 56.
36. Örnung G., Shupliakow O., Ottersen O. P., Storm-Mathisen J. Cullheim S. (1994) Immunohistochemical evidence for coexistence of glycine and GABA in nerve terminals on cat spinal motorneurones: an ultrastructural study. NeuroReport 5, 889 892.
37. Poyatos I., Ponce J., Aragón C., Giménez C. Zafra F. (1997) The glycine transporter GLYT2 is a reliable marker for glycine-immunoreactive neurons. Mol. Brain Res. 49, 63 70.
38. Raiteri L. Raiteri M. (2000) Synaptosomes still viable after 25 years of superfusion. Neurochem. Res. 25, 1265 1274.
39. +]: involvement of transporter reversal and mitochondrial calcium. J. Neurochem. 80, 706 714.
40. Raiteri L., Stigliani S., Siri A., Passalacqua M., Melloni E., Raiteri M. Bonanno G. (2005) Glycine taken up through GLYT1 and GLYT2 heterotransporters into glutamatergic axon terminals of mouse spinal cord elicits release of glutamate by homotransporter reversal and through anion channels. Biochem. Pharmacol. 69, 159 168.
41. Rizzuto R., Bernardi P. Pozzan T. (2000) Mitochondria as all-round players of the calcium game. J. Physiol. 529, 37 47.
42. Roseth S., Fykse E. M. Fonnum F. (1995) Uptake of l-glutamate into rat brain synaptic vesicles: effect of inhibitors that bind specifically to the glutamate transporter. J. Neurochem. 65, 96 103.
43. Roux M. J. Supplisson S. (2000) Neuronal and glial glycine transporters have different stoichiometries. Neuron 25, 373 383.
44. Schiavo G., Shone C. C., Bennett M. K., Scheller R. H. Montecucco C. (1995) Botulinum neurotoxin type C cleaves a single Lys-Ala bond within the carboxyl-terminal region of syntaxin. J. Biol. Chem. 270, 10566 10570.
45. Spike R. C., Watt C., Zafra F. Todd A. J. (1997) An ultrastructural study of the glycine transporter GLYT2 and its association with glycine in the superficial laminae of the rat spinal dorsal horn. Neuroscience 77, 543 551.
46. Stigliani S., Zappettini S., Raiteri L. et al. (2006) Glia re-sealed particles freshly prepared from adult rat brain are competent for exocytotic release of glutamate. J. Neurochem. 96, 656 668.
47. 2+ exchange inhibitor CGP-37157 in rat atrial myocytes. Eur. J. Pharmacol. 552, 15 19.
48. Todd A. J. Spike R. C. (1993) The localization of classical transmitters and neuropeptides within neurons in laminae I-III of the mammalian spinal dorsal horn. Prog. Neurobiol. 41, 609 645.
49. Todd A. J., Watt C., Spike R. C. Sieghart W. (1996) Colocalization of GABA, glycine, and their receptors at synapses in the rat spinal cord. J. Neurosci. 16, 974 982.
50. Williamson L. C., Halpern J. L., Montecucco C., Brown J. E. Neale E. A. (1996) Clostridial neurotoxins and substrate proteolysis in intact neurons. J. Biol. Chem. 271, 7694 7699.
51. Zafra F., Aragón C., Olivares L., Danbolt N. C., Giménez C. Storm-Mathisen J. (1995a) Glycine transporters are differentially expressed among CNS cells. J. Neurosci. 15, 3952 3969.
52. Zafra F., Gomeza J., Olivares L., Aragón C. Giménez C. (1995b) Regional distribution and developmental variation of the glycine transporters GLYT1 and GLYT2 in the rat CNS. Eur. J. Neurosci. 7, 1342 1352.
53. Zeilhofer H. U. (2005) The glycinergic control of spinal pain processing. Cell. Mol. Life Sci. 62, 2027 2035.