Feasibility of a molecular screening method for detection of Salmonella enterica and Campylobacter jejuni in a routine community-based clinical microbiology laboratory

Journal of Clinical Microbiology

Volumn 45, Issue 11, 2007, Pages 3692-3700

  Schuurman, T ^a,c   De Boer, R F ^a   Van Zanten, E ^a   Van Slochteren, K R ^a   Scheper, H R ^b   Dijk Alberts, B G ^b   Moller A V M ^b   Kooistra Smid, A M D ^a  

^a Laboratory for Infectious Diseases ^*  (Netherlands)

^b Laboratory for Infectious Diseases   (Netherlands)

^c UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; CAMPYLOBACTER JEJUNI; CLINICAL LABORATORY; CONTROLLED STUDY; DIAGNOSTIC PROCEDURE; FEASIBILITY STUDY; FECES; GENE TARGETING; NONHUMAN; PRIORITY JOURNAL; PROSPECTIVE STUDY; REAL TIME POLYMERASE CHAIN REACTION; SALMONELLA ENTERICA; SCREENING TEST; SENSITIVITY AND SPECIFICITY;

CAMPYLOBACTER JEJUNI; DNA, BACTERIAL; FECES; HUMANS; LABORATORIES; POLYMERASE CHAIN REACTION; SALMONELLA ENTERICA; TIME FACTORS;

BACTERIA (MICROORGANISMS); CAMPYLOBACTER JEJUNI; SALMONELLA ENTERICA;

EID: 36348940547     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00896-07     Document Type: Article

References (38)

1. Abu Al-Soud, W., and P. Rådström. 1998. Capacity of nine thermostable DNA polymerases to mediate amplification in the presence of PCR-inhibiting samples. Appl. Environ. Microbiol. 64:3748-3753.
2. Abu Al-Soud, W., and P. Rådström. 2000. Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat. J. Clin. Microbiol. 38:4463-4470.
3. Allos, B. M. 1997. Association between Campylobacter infection and Guillain-Barré syndrome. J. Infect. Dis. 176:S125-S128.
4. Beld, M., R. Minnaar, J. Weel, C. Sol, M. Damen, H. van der Avoort, P. Wertheim-van Dillen, A. van Breda, and R. Boom. 2004. Highly sensitive assay for detection of enterovirus in clinical specimens by reverse transcription-PCR with an armored RNA internal control. J. Clin. Microbiol. 42: 3059-3064.
5. Best, E. L., E. J. Powell, C. Swift, K. A. Grant, and J. A. Frost. 2003. Applicability of a rapid duplex real-time PCR assay for speciation of Campylobacter jejuni and Campylobacter coli directly from culture plates. FEMS Microbiol. Lett. 229:237-241.
6. Boom, R., C. Sol, J. Weel, K. Lettinga, Y. Gerrits, A. van Breda, and P. Wertheim-Van Dillen. 2000. Detection and quantitation of human cytomegalovirus DNA in faeces. J. Virol. Methods 84:1-14.
7. Chiu, C.-H., and J. T. Ou. 1996. Rapid identification of Salmonella serovars in feces by specific detection of virulence genes, invA and invC, by an enrichment broth culture-multiplex PCR combination assay. J. Clin. Microbiol. 34:2619-2622.
8. Denis, M., C. Soumet, K. Rivoal, G. Ermel, D. Blivet, G. Salvat, and P. Colin. 1999. Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli. Lett. Appl. Microbiol. 29:406-410.
9. De Wit, M. A. S., M. P. G. Koopmans, L. M. Kortbeek, N. J. van Leeuwen, A. I. M. Bartelds, and Y. T. H. P. van Duynhoven. 2001. Gastroenteritis in sentinel general practices, The Netherlands. Emerg. Infect. Dis. 7:82-89.
10. De Wit, M. A. S., M. P. G. Koopmans, L. M. Kortbeek, N. J. van Leeuwen, J. Vinjé, and Y. T. H. P. van Duynhoven. 2001. Etiology of gastroenteritis in sentinel general practices in The Netherlands. Clin. Infect. Dis. 33:280-288.
11. Dusch, H., and M. Altwegg. 1995. Evaluation of five new plating media for isolation of Salmonella species. J. Clin. Microbiol. 33:802-804.
12. Englen, M. D., and P. J. Fedorka-Cray. 2002. Evaluation of a commercial diagnostic PCR for the identification of Campylobacter jejuni and Campylobacter coli. Lett. Appl. Microbiol. 35:353-356.
13. Farrell, J. J., L. J. Doyle, R. M. Addison, L. B. Reller, G. S. Hall, and G. W. Procop. 2005. Broad-range (pan) Salmonella and Salmonella serotype Typhi-specific real-time PCR assays. Am. J. Clin. Pathol. 123:339-345.
14. Guarrant, R. L., J. M. Hughes, N. L. Lima, and J. Crane. 1990. Diarrhea in developed and developing countries: magnitude, special settings, and etiologies. Rev. Infect. Dis. 12:S41-S50.
15. Holland, J. J., L. Louie, A. E. Simor, and M. Louie. 2000. PCR detection of Escherichia coli O157:H7 directly from stools: evaluation of commercial extraction methods for purifying fecal DNA. J. Clin. Microbiol. 38:4108-4113.
16. Hoorfar, J., P. Ahrens, and P. Rådström. 2000. Automated 5′ nuclease PCR assay for identification of Salmonella enterica. J. Clin. Microbiol. 38:3429-3435.
17. Iijima, Y., N. T. Asako, M. Aihara, and K. Hayashi. 2004. Improvement in the detection rate of diarrhoeagenic bacteria in human stool specimens by a rapid real-time PCR assay. J. Med. Microbiol. 53:617-622.
18. Jensen, A. N., M. T. Andersen, A. Dalsgaard, D. L. Baggesen, and E. M. Nielsen. 2005. Development of real-time PCR and hybridization methods for detection and identification of thermophilic Campylobacter spp. in pig faecal samples. J. Appl. Microbiol. 99:292-300.
19. Kolackova, I., and R. Karpiskova. 2005. Species level identification of thermotolerant campylobacters. Vet. Med. Czech. 50:543-547.
20. Kramer, J. M., J. A. Frost, D. R. A. Wareing, and F. J. Bolton. 2000. Campylobacter contamination of raw meat and poultry at retail sale: identification of multiple types and comparison with isolates from human infection. J. Food Prot. 63:1654-1659.
21. Kreader, C. A. 1996. Relief of amplification inhibition in PCR with bovine serum albumin or T4 gene 32 protein. J. Clin. Microbiol. 34:1102-1106.
22. Kulkarni, S. P., S. Lever, J. M. J. Logan, A. J. Lawson, J. Stanley, and M. S. Shafi. 2002. Detection of Campylobacter species: a comparison of culture and polymerase chain reaction based methods. J. Clin. Pathol. 55:749-753.
23. LaGier, M. J., L. A. Joseph, T. V. Passaretti, K. A. Musser, and N. M. Cirino. 2004. A real-time multiplexed PCR assay for rapid detection and differentiation of Campylobacter jejuni and Campylobacter coli. Mol. Cell. Probes 18:275-282.
24. Lawson, A. J., J. M. J. Logan, G. L. O'Neill, M. Desal, and J. Stanley. 1999. Large-scale survey of Campylobacter species in human gastroenteritis by PCR and PCR-enzyme-linked immunosorbent assay. J. Clin. Microbiol. 37:3860-3864.
25. Logan, J. M. J., K. J. Edwards, N. A. Saunders, and J. Stanley. 2001. Rapid identification of Campylobacter spp. by melting peak analysis of biprobes in real-tme PCR. J. Clin. Microbiol. 39:2227-2232.
26. Maher, M., C. Finnegan, E. Collins, B. Ward, C. Carroll, and M. Cormican. 2003. Evaluation of culture methods and a DNA probe-based PCR assay for detection of Campylobacter species in clinical specimens of feces. J. Clin. Microbiol. 41:2980-2986.
27. Malorny, B., E. Paccassoni, P. Fach, C. Bunge, A. Martin, and R. Helmuth. 2004. Diagnostic real-time PCR for the detection of Salmonella in food. Appl. Environ. Microbiol. 70:7046-7052.
28. Monteiro, L., D. Bonnemaison, A. Vekris, K. G. Petry, J. Bonnet, R. Vidal, J. Cabrita, and F. Megraud. 1997. Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model. J. Clin. Microbiol. 35:995-998.
29. Nogva, H. K., A. Bergh, A. Holck, and K. Rudi. 2000. Application of the 5′-nuclease PCR assay in evaluation and development of methods for quantitative detection of Campylobacter jejuni. Appl. Environ. Microbiol. 66: 4029-4036.
30. Rudi, K., H. K. Høidal, T. Katla, B. K. Johansen, J. Nordal, and K. S. Jakobsen. 2004. Direct real-time PCR quantification of Campylobacter jejuni in chicken fecal and cecal samples by integrated cell concentration and DNA purification. Appl. Environ. Microbiol. 70:790-797.
31. Ruiz, J., M. L. Nunez, J. Diaz, I. Lorente, J. Perez, and J. Gomez. 1996. Comparison of five plating media for isolation of Salmonella species from human stools. J. Clin. Microbiol. 34:686-688.
32. Schuurman, T., A. Roovers, W. K. van der Zwaluw, A. A. van Zwet, L. J. M. Sabbe, A. M. D. Kooistra-Smid, and Y. T. H. P. van Duynhoven. 2007. Evaluation of 5′-nuclease and hybridization probe assays for the detection of Shiga toxin-producing Escherichia coli in human stools. J. Microbiol. Methods doi:10.1016/j.mimet. 2007.05.016.
33. Van Belkum, A., N. van den Braak, P. Godschalk, W. Ang, B. Jacobs, and M. Gilbert. 2001. A Campylobacter jejuni gene associated with immune-mediated neuropathy. Nat. Med. 7:752-753.
34. van den Brandhof, W. E., G. A de Wit, M. A. S. de Wit, and Y. T. H. P. van Duynhoven. 2004. Costs of gastroenteritis in The Netherlands. Epidemiol. Infect. 132:211-221.
35. Van Hees, B. C., M. J. Veldman-Ariesen, B. M. de Jongh, M. Tersmette, and W. van Pelt. 2007. Regional and seasonal differences in incidence and antibiotic resistance of Campylobacter from a nationwide surveillance study in The Netherlands: an overview of 2000-2004. Clin. Microbiol. Infect. 13:305-310.
36. Van Pelt, W., M. A. de Wit, W. J. Wannet, E. J. Ligtvoet, M. A. Widdowson, and Y. T. van Duynhoven. 2003. Laboratory surveillance of bacterial gastroenteric pathogens in The Netherlands, 1991-2001. Epidemiol. Infect. 130: 431-441.
37. Widjojoatmodjo, M. N., A. C. Fluit, R. Torensma, G. P. H. Verdonk, and J. Verhoef. 1992. The magnetic immuno polymerase chain reaction assay for direct detection of Salmonellae in fecal samples. J. Clin. Microbiol. 30:3195-3199.
38. Yuki, N. 2001. Infectious origins of, and molecular mimicry in, Guillain-Barré and Fisher syndromes. Lancet Infect. Dis. 1:29-37.