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Volumn 318, Issue 5853, 2007, Pages 1136-1140

Widespread monoallelic expression on human autosomes

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKINE; IMMUNOGLOBULIN; NATURAL KILLER CELL RECEPTOR; T LYMPHOCYTE RECEPTOR;

EID: 36249017308     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1148910     Document Type: Article
Times cited : (469)

References (30)
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    • Materials and methods are available as supporting material on Science Online.
    • Materials and methods are available as supporting material on Science Online.
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    • We limited our analyses to SNPs that were heterozygous in genomic DNA (from a given individual) and that were consistent with Mendelian inheritance of the parental genotypes. As an additional control, we verified heterozygosity in genomic DNA from each clone. When analyzing cDNA genotypes, we eliminated all SNPs for which we consistently detected only one of the two alleles or for which only one allele was detected in the nonclonal cell line, A further filter was to consider only SNPs that gave a cDNA genotype call for at least two distinct clones
    • We limited our analyses to SNPs that were heterozygous in genomic DNA (from a given individual) and that were consistent with Mendelian inheritance of the parental genotypes. As an additional control, we verified heterozygosity in genomic DNA from each clone. When analyzing cDNA genotypes, we eliminated all SNPs for which we consistently detected only one of the two alleles (or for which only one allele was detected in the nonclonal cell line). A further filter was to consider only SNPs that gave a cDNA genotype call for at least two distinct clones.
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    • Although the data we present are from the 260,000 SNPs present on the Affymetrix Nsp 250 K mapping array, we have also obtained qualitatively similar data from the Sty 250 K mapping array and from the earlier generation 100 K mapping array set
    • Although the data we present are from the 260,000 SNPs present on the Affymetrix Nsp 250 K mapping array, we have also obtained qualitatively similar data from the Sty 250 K mapping array and from the earlier generation 100 K mapping array set.
  • 17
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    • Several SNPs, particularly at the distal portion of the short arm, showed biallelic expression. The locations of these SNPs fell within areas known to contain genes that escape X-inactivation
    • Several SNPs, particularly at the distal portion of the short arm, showed biallelic expression. The locations of these SNPs fell within areas known to contain genes that escape X-inactivation.
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    • For help with microarray experiments, we thank S. Levy at the Vanderbilt Microarray Shared Resource, V. Morgan at the Harvard Medical School-Partners Healthcare Center for Genetics and Genomics Microarray Facility, and B. Blumenstiel and S. Gabriel at the Broad Institute Center for Genotyping and Analysis. We thank A. Ensminger, M. Tackett, and M. Tipping for help in subcloning cell lines; D. Lucente for help with in vivo experiments; and D. Bartel, A. Bortvin, R. Medzhitov, V. Mootha, P. Sklar, and members of the lab for comments on the manuscript. This work was supported by NIH grants to A.C.
    • For help with microarray experiments, we thank S. Levy at the Vanderbilt Microarray Shared Resource, V. Morgan at the Harvard Medical School-Partners Healthcare Center for Genetics and Genomics Microarray Facility, and B. Blumenstiel and S. Gabriel at the Broad Institute Center for Genotyping and Analysis. We thank A. Ensminger, M. Tackett, and M. Tipping for help in subcloning cell lines; D. Lucente for help with in vivo experiments; and D. Bartel, A. Bortvin, R. Medzhitov, V. Mootha, P. Sklar, and members of the lab for comments on the manuscript. This work was supported by NIH grants to A.C.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.