Tertiary structure in 7.9 M guanidinium chloride - The role of Glu53 and Asp287 in Pyrococcus furiosus endo-β-1,3-glucanase

FEBS Journal

Volumn 274, Issue 23, 2007, Pages 6167-6179

  Chiaraluce, Roberta ^a   Florio, Rita ^a   Angelaccio, Sebastiana ^a   Gianese, Giulio ^b   Van Lieshout, Johan F T ^c   Van Der Oost, John ^c   Consalvi, Valerio ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b ENEA C R CASACCIA   (Italy)

^c WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Double mutant; Glycoside hydrolases; Laminarinase; Protein stability; Thermodynamic stability

Indexed keywords

8 ANILINO 1 NAPHTHALENESULFONIC ACID; ACRYLAMIDE; ASPARTIC ACID; CALCIUM; ENDO 1,3 GLUCANASE; GLUTAMIC ACID; GLYCOSIDASE; GUANIDINE; UNCLASSIFIED DRUG;

ARTICLE; CALCIUM BINDING; CONTROLLED STUDY; FLUORESCENCE; GENE MUTATION; NONHUMAN; PHOTOCHEMICAL QUENCHING; PRIORITY JOURNAL; PROTEIN STRUCTURE; PYROCOCCUS FURIOSUS; SPECTROSCOPY; SUBSTITUTION REACTION; THERMODYNAMICS; THERMOSTABILITY;

ANILINO NAPHTHALENESULFONATES; ASPARTIC ACID; BINDING SITES; CALCIUM; CHELATING AGENTS; DOSE-RESPONSE RELATIONSHIP, DRUG; EGTAZIC ACID; ENZYME STABILITY; ESCHERICHIA COLI; FLUORESCENT DYES; GLUCAN ENDO-1,3-BETA-D-GLUCOSIDASE; GLUTAMIC ACID; GUANIDINE; KINETICS; MODELS, BIOLOGICAL; MODELS, MOLECULAR; MUTATION; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN RENATURATION; PROTEIN STRUCTURE, TERTIARY; PYROCOCCUS FURIOSUS; THERMODYNAMICS; TITRIMETRY;

ARCHAEA; PYROCOCCUS FURIOSUS;

EID: 36248968590     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2007.06137.x     Document Type: Article

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