A method to determine 18O kinetic isotope effects in the hydrolysis of nucleotide triphosphates

Analytical Biochemistry

Volumn 372, Issue 2, 2008, Pages 213-221

  Du, Xinlin ^a   Ferguson, Kurt ^b   Gregory, Robert ^b   Sprang, Stephen R ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b SOUTHERN METHODIST UNIVERSITY   (United States)

Author keywords

Chemical reaction interface (CRI); Enzyme mechanism; GTP hydrolysis; GTPase activating protein (GAP); Isotope ratio mass spectrometer (IRMS); Kinetic isotope effect (KIE); Ras; Transition state

Indexed keywords

CARBON; HYDROLYSIS; INTERFACE STATES; KINETICS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETERS; NUCLEOTIDES; RADIUM; SUBSTRATES;

ENZYME MECHANISM; GTP HYDROLYSIS; GTPASE-ACTIVATING PROTEIN; ISOTOPE RATIO MASS SPECTROMETER; KINETIC ISOTOPE EFFECTS; TRANSITION STATE;

ISOTOPES;

CARBON; CARBON 13; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; GUANOSINE TRIPHOSPHATE; NUCLEOTIDE DERIVATIVE; OXYGEN 18; RAS PROTEIN;

ACCURACY; ARTICLE; CATALYSIS; ENZYME MECHANISM; HYDROLYSIS; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PRIORITY JOURNAL; REACTION ANALYSIS;

EID: 36148951744     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.09.013     Document Type: Article

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