Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters

Biophysical Journal

Volumn 93, Issue 9, 2007, Pages 3285-3290

  Egner, Alexander ^a   Geisler, Claudia ^a   Von Middendorff, Claas ^a   Bock, Hannes ^a   Wenzel, Dirk ^a   Medda, Rebecca ^a   Andresen, Martin ^a   Stiel, Andre C ^a   Jakobs, Stefan ^a   Eggeling, Christian ^a   Schönle, Andreas ^a   Hell, Stefan W ^a  

^a MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; FLUORESCENCE MICROSCOPY; MAMMAL CELL; MICROTUBULE; NANOTECHNOLOGY; NONHUMAN; PHOTON;

MAMMALIA;

EID: 35948950088     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.107.112201     Document Type: Article

References (24)

1. Hell, S. W. 1994. Improvement of lateral resolution in far-field light microscopy using two-photon excitation with offset beams. Opt. Commun. 106:19-22.
2. Hell, S. W., and J. Wichmann. 1994. Breaking the diffraction resolution limit by stimulated emission: stimulated emission depletion microscopy. Opt. Lett. 19:780-782.
3. Hell, S. 2003. Toward fluorescence nanoscopy. Nat. Biotechnol. 21:1347-1355.
4. Bobroff, N. 1986. Position measurement with a resolution and noise-limited instrument. Rev. Sci. Instrum. 57:1152-1157.
5. Betzig, E. 1995. Proposed method for molecular optical imaging. Opt. Lett. 20:237-239.
6. Hell, S. W., J. Soukka, and P. E. Hänninen. 1995. Two- and multiphoton detection as an imaging mode and means of increasing the resolution in far-field light microscopy. Bioimaging. 3:65-69.
7. Schmidt, T., G. J. Schutz, W. Baumgartner, H. J. Gruber, and H. Schindler. 1996. Imaging of single molecule diffusion. Proc. Natl. Acad. Sci. USA. 93:2926-2929.
8. Lacoste, T., X. Michalet, F. Pinaud, D. Chemla, A. Alivisatos, and S. Weiss. 2000. Ultrahigh-resolution multicolor colocalization of single fluorescent probes. Proc. Natl. Acad. Sci. USA. 97:9461-9466.
9. Michalet, X., T. D. Lacoste, and S. Weiss. 2001. Ultrahigh-resolution colocalization of spectrally separable point-like fluorescent probes. Methods. 25:87-102.
10. Betzig, E., G. Patterson, R. Sougrat, O. Lindwasser, S. Olenych, J. Bonifacino, M. Davidson, J. Lippincott-Schwartz, and H. Hess. 2006. Imaging intracellular fluorescent proteins at nanometer resolution. Science. 313:1642-1645.
11. Hess, S. T., T. P. K. Girirajan, and M. D. Mason. 2006. Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys. J. 91:4258-4272.
12. Rust, M. J., M. Bates, and X. Zhuang. 2006. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat. Methods. 3:793-796.
13. Hell, S. W. 2007. Far-field optical nanoscopy. Science. 316:1153-1158.
14. Moerner, W. E. 2006. Single-molecule mountains yield nanoscale cell images. Nat. Methods. 3:781-782.
15. Stiel, A. C., S. Trowitzsch, G. Weber, M. Andresen, C. Eggeling, S. W. Hell, S. Jakobs, and M. C. Wahl. 2007. 1.8 Å bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants. Biochem. J. 402:35-42.
16. Ando, R., H. Mizuno, and A. Miyawaki. 2004. Regulated fast nucleo-cytoplasmic shuttling observed by reversible protein highlighting. Science. 306:1370-1373.
17. Geisler, C., A. Schönle, C. von Middendorff, H. Bock, C. Eggeling, A. Egner, and S. W. Hell. 2007. Resolution of λ/10 in fluorescence microscopy using fast single molecule photo-switching. Appl. Phys. A. 88:223-226.
18. Hogbom, J. 1974. Aperture synthesis with a non-regular distribution of interferometer baselines. Astrophys. J. Suppl. Ser. 15:417-426.
19. Thompson, R., D. Larson, and W. Webb. 2002. Precise nanometer localization analysis for individual fluorescent probes. Biophys. J. 82: 2775-2783.
20. Drew, D. E., G. von Heijne, P. Nordlund, and J. W. de Gier. 2001. Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli. FEBS Lett. 507:220-224.
21. Tokuyasu, K. T. 1973. A technique for ultracryotomy of cell suspensions and tissues. J. Cell Biol. 57:551-565.
22. Tokuyasu, K. T. 1980. Immunochemistry on ultrathin frozen sections. Histochem. J. 12:381-403.
23. Liou, W., H. J. Geuze, and J. W. Slot. 1996. Improving structural integrity of cryosections for immunogold labeling. Histochem. Cell Biol. 106:41-58.
24. Osborn, M., W. W. Franke, and K. Weber. 1977. Visualization of a system of filaments 7-10 nm thick in cultured cells of an epithelioid line (Pt K2) by immunofluorescence microscopy. Proc. Natl. Acad. Sci. USA. 74:2490-2494.