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35848965533
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The velocity field of the species F can be essentially considered as constant along 2 in the whole device (vide infra). As the F concentration at the injection point is also independent on z, the problem is rigorously bidimensional.
-
The velocity field of the species F can be essentially considered as constant along 2 in the whole device (vide infra). As the F concentration at the injection point is also independent on z, the problem is rigorously bidimensional.
-
-
-
-
25
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35848964429
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2t).
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2t).
-
-
-
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26
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13244269742
-
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For an example of synthesis protocol, see
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For an example of synthesis protocol, see: Cadix, A; Chassenieux, C.; Lafuma, F.; Lequeux, F. Macromolecules 2005, 38, 527-536.
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31
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0036111913
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The front position was calculated by correlating each intensity profile with its symetric. See, for example
-
The front position was calculated by correlating each intensity profile with its symetric. See, for example: Gosse, C.; Croquette, V. Biophys. J. 2002, 82, 3314-3329.
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Gosse, C.1
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32
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35848957521
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j,n extraction.
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j,n extraction.
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33
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35848936990
-
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The whole pool of normalized Fourier modes was appropriate for mixture analysis. All the normalized Fourier modes are fitted in parallel with a monoexponential and a biexponential function. All the modes giving satisfactory residuals with the simpler fit were discarded, the parameters extracted from their biexponential analysis being considered unreliable (Figure S-9).
-
The whole pool of normalized Fourier modes was appropriate for mixture analysis. All the normalized Fourier modes are fitted in parallel with a monoexponential and a biexponential function. All the modes giving satisfactory residuals with the simpler fit were discarded, the parameters extracted from their biexponential analysis being considered unreliable (Figure S-9).
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-
-
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34
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35848961953
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The Gaussian shape reflects the channel one broadened by molecular diffusion and migration due to out-of-axis electric fields between the injection nozzle and the zone of homogeneous velocity see Supporting Information
-
The Gaussian shape reflects the channel one broadened by molecular diffusion and migration due to out-of-axis electric fields between the injection nozzle and the zone of homogeneous velocity (see Supporting Information).
-
-
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35
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2642510833
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Gosse, C.; Boutorine, A.; Aujard, I.; Chami, M.; Kononov, A.; Cogné-Laage, E.; Alemand, J.-F.; Li, J.; Jullien, L. J. Phys. Chem. B 2004, 108, 6485-6497.
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Kononov, A.5
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Jullien, L.9
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0035716502
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Cox, E.C.6
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39
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35848943943
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The bowed channels located on the injection side of the device were shortened to compensate for width reduction, thus keeping their overall resistance constant
-
The bowed channels located on the injection side of the device were shortened to compensate for width reduction, thus keeping their overall resistance constant.
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-
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40
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35848931938
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Electric potential profiles were calculated by finite element method simulations and found in good agreement with this estimate. The electric field is constant and directed along the x-axis everywhere beyond a l0, 40 μm limit inside the chamber boundaries Figure S-7, We checked on chip these predictions by recording the migration of long and thin bands of YOYO-1-stained 1257-bp dsDNA When prepared along y and eluted in the x direction, a stripe of this weakly diffusing fluorophore moves straight and stays normal to u→x, thus confirming the field homogeneity and direction
-
x, thus confirming the field homogeneity and direction.
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-
-
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41
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35848933301
-
-
The pillars that sustain the PDMS chamber ceiling also induce a perturbation of the electric potential. However, it is of the order of their diameter (i.e, 30 μm) and exerts no significant influence on the analyte migration
-
The pillars that sustain the PDMS chamber ceiling also induce a perturbation of the electric potential. However, it is of the order of their diameter (i.e., 30 μm) and exerts no significant influence on the analyte migration.
-
-
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43
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0036672775
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Doherty, E. A S.; Berglund, K D.; Buchholz, B. A; Kourkine, I. V.; Przybycien; T. M.; Tilton, R D.; Barron, A. E. Electrophoresis 2002, 23, 2766-2776.
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0002441714
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1242328691
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By comparing the fluorescein electrophoretic velocity in the two media, the remaining electroosmotic mobility μEO in 0.1, PDMA was evaluated to be less than 10% of the apparent electrophoretic mobility for all the considered analytes; see ref 43 and: Zhang, P, Ren, J. Anal Chim. Acta 2004, 507, 179-184
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EO in 0.1 % PDMA was evaluated to be less than 10% of the apparent electrophoretic mobility for all the considered analytes; see ref 43 and: Zhang, P.; Ren, J. Anal Chim. Acta 2004, 507, 179-184.
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-
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48
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35848953250
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To quantify the electroosmotic dispersive contribution that could superimpose to Brownian diffusion, we designed a series of control time-based experiments where the apparent diffusion coefficients are extracted from the time evolution along the y-axis of an x-invariant concentration profile. Measurements were performed either in the absence or in the presence of a transverse alternative electric field Ey (see Supporting Information).7,45 For the two filling media, the good agreement between the apparent diffusion coefficients extracted from time-based as well as continuous experiments strongly supports the absence of any significant electric field-induced dispersion in the present systems
-
7,45 For the two filling media, the good agreement between the apparent diffusion coefficients extracted from time-based as well as continuous experiments strongly supports the absence of any significant electric field-induced dispersion in the present systems.
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49
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35848932138
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As evaluated from Mark-Houwink parameters (Heller, C. Electrophoresis 1999, 20, 1962-1977, 0.1, w/w) PDMA approximately corresponds to the entanglement threshold for the 3-MDa polymer. Thus, slight viscosity or steric effects might be present
-
As evaluated from Mark-Houwink parameters (Heller, C. Electrophoresis 1999, 20, 1962-1977), 0.1 % (w/w) PDMA approximately corresponds to the entanglement threshold for the 3-MDa polymer. Thus, slight viscosity or steric effects might be present.
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0141885303
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0034671973
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In the present approach, the differentiation between two species Fj relies on their respective Dj/ν xj ratio. Using the Einstein equation Dj, kT/ξj, the expressions of the electrophoretic mobility μj, zj/ξj and of the velocity ν→j, νxju→ x, μj, μEO Exu→x, this discriminating factor develops as Dj/νxj, kT/[(zj, μEO ξj)Ex, where ξj and zj are the respective Fj solute molecular friction coefficient and charge. In principle, it should consequently always be possible to discriminate the two components in a binary mixture. Indeed, even when
-
j.
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58
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35848949136
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A sum of two Gaussian functions failed to fit the stationary migration, diffusion pattern in the direct space, thus emphasizing the strength of Fourier data analysis
-
A sum of two Gaussian functions failed to fit the stationary migration - diffusion pattern in the direct space, thus emphasizing the strength of Fourier data analysis.
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59
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35848931283
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From independent bulk measurements, we estimated the chemical relaxation time for all the injected duplexes to be at least 1 order of magnitude longer than the analysis duration. Consequently, the present technique provides information on the composition of the reactive mixture, which can be considered as frozen at equilibrium.
-
From independent bulk measurements, we estimated the chemical relaxation time for all the injected duplexes to be at least 1 order of magnitude longer than the analysis duration. Consequently, the present technique provides information on the composition of the reactive mixture, which can be considered as frozen at equilibrium.
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-
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60
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0022423486
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Aboul-ela, F.; Koh, D.; Tinoco, I., Jr.; Martin, F. H. Nucleic Acids Res. 1985, 13, 4811-4825.
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