Modulation of the amplitude of NAD(P)H fluorescence transients after synaptic stimulation

Journal of Neuroscience Research

Volumn 85, Issue 15, 2007, Pages 3233-3243

  Brennan, A M ^a   Connor, J A ^a   Shuttleworth, C W ^a  

^a UNIVERSITY OF NEW MEXICO SCHOOL OF MEDICINE   (United States)

Author keywords

Brain slice; Hippocampus; Imaging; Mitochondria; NADH fluorescence

Indexed keywords

REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ANIMAL TISSUE; BRAIN REGION; BRAIN SLICE; CONFERENCE PAPER; CONTROLLED STUDY; ELECTROSTIMULATION; FLUORESCENCE; MALE; MITOCHONDRIAL RESPIRATION; MOUSE; NONHUMAN; PRIORITY JOURNAL; STIMULUS RESPONSE; SYNAPTIC POTENTIAL;

ANIMALS; BRAIN; ELECTRIC STIMULATION; FLAVOPROTEINS; FLUORESCENCE; MALE; MICE; MICE, INBRED C57BL; MITOCHONDRIA; NADP; ORGAN CULTURE TECHNIQUES; SYNAPSES;

EID: 35848954074     PISSN: 03604012     EISSN: 10974547     Source Type: Journal    
DOI: 10.1002/jnr.21288     Document Type: Conference Paper

References (27)

1. Anderson RE, Meyer FB. 2002. In vivo fluorescent imaging of NADH redox state in brain. Methods Enzymol 352:482-494.
2. Bachelard HS, Cox DW, Drower J. 1984. Sensitivity of guinea-pig hippocampal granule cell field potentials to hexoses in vitro: an effect on cell excitability? J Physiol 352:91-102.
3. Borowsky IW, Collins RC. 1989a. Metabolic anatomy of brain: a comparison of regional capillary density, glucose metabolism, and enzyme activities. J Comp Neurol 288:401-413.
4. Borowsky IW, Collins RC. 1989b. Histochemical changes in enzymes of energy metabolism in the dentate gyrus accompany deafferentation and synaptic reorganization. Neuroscience 33:253-262.
5. Brennan AM, Connor JA, Shutdeworth CW. 2006. NAD(P)H fluorescence transients after synaptic activity in brain slices: predominant role of mitochondrial function. J Cereb Blood Flow Metab 26:1389-1406.
6. Chance B. 2004. Mitochondrial NADH redox state, monitoring discovery and deployment in tissue. Methods Enzymol 385:361-370.
7. Chance B, Cohen P, Jobsis F, Schoener B. 1962. Intracellular oxidation-reduction states in vivo. Science 137:499-508.
8. Dora E, Gyulai L, Kovach AG. 1984. Determinants of brain activation-induced cortical NAD/NADH responses in vivo. Brain Res 299:61-72.
9. Duchen MR. 1992. Ca(2+)-dependent changes in the mitochondrial energetics in single dissociated mouse sensory neurons. Biochem J 283:41-50.
10. Estabrook RW. 1962. Fluorometric measurement of reduced pyridine nucleotide in cellular and subcellular particles. Anal Biochem 4:231-245.
11. Foster KA, Beaver CJ, Turner DA. 2005. Interaction between tissue oxygen tension and NADH imaging during synaptic stimulation and hypoxia in rat hippocampal slices. Neuroscience 132:645-657.
12. Kageyama GH, Wong-Rjley MT. 1982. Histochemical localization of cytochrome oxidase in the hippocampus: correlation with specific neuronal types and afferent pathways. Neuroscience 7:2337-2361.
13. Kann O, Schuchmann S, Buchheim K, Heinemann U. 2003. Coupling of neuronal activity and mitochondrial metabolism as revealed by NAD(P)H fluorescence signals in organotypic hippocampal slice cultures of the rat. Neuroscience 119:87-100.
14. Kasischke KA, Vishwasrao HD, Fisher PJ, Zipfel WR, Webb WW. 2004. Neural activity triggers neuronal oxidative metabolism followed by astrocytic glycolysis. Science 305:99-103.
15. Kovacs R, Schuchmann S, Gabriel S, Kann O, Kardos J, Heinemann U. 2002. Free radical-mediated cell damage after experimental status epilepticus in hippocampal slice cultures. J Neurophysiol 88:2909-2918.
16. Lipton P. 1973. Effects of membrane depolarization on nicotinamide nucleotide fluorescence in brain slices. Biochem J 136:999-1009.
17. Mayevsky A. 1984. Brain NADH redox state monitored in vivo by fiber optic surface fluorometry. Brain Res 319:49-68.
18. Mironov SL, Richter DW. 2001. Oscillations and hypoxic changes of mitochondrial variables in neurons of the brainstem respiratory centre of mice. J Physiol 533:227-236.
19. Nicholls DG. 2005. Mitochondria and calcium signaling. Cell Calcium 38:311-317.
20. Reinert KC, Dunbar RL, Gao W, Chen G, Ebner TJ. 2004. Flavoprotein autofluorescence imaging of neuronal activation in the cerebellar cortex in vivo. J Neurophysiol 92:199-211.
21. Schuchmann S, Buchheim K, Meierkord H, Heinemann U. 1999. A relative energy failure is associated with low-Mg2+ but not with 4-aminopyridine induced seizure-like events in entorhinal cortex. J Neurophysiol 81:399-403.
22. Schuchmann S, Kovacs R, Kann O, Heinemann U, Buchheim K. 2001. Monitoring NAD(P)H autofluorescence to assess mitochondrial metabolic functions in rat hippocampal-entorhinal cortex slices. Brain Res Brain Res Protoc 7:267-276.
23. Shibuki K, Hishida R, Murakami H, Kudoh M, Kawaguchi T, Watanabe M, Watanabe S, Kouuchi T, Tanaka R. 2003. Dynamic imaging of somatosensory cortical activity in the rat visualized by flavoprotein autofluorescence. J Physiol 549:919-927.
24. Shuttleworth CW, Brennan AM. 2005. Modification of NAD(P)H dynamics by ongoing activity in brain slices. Soc Neuroscience Abstracts 188.4.
25. Shuttleworth CW, Brennan AM, Connor JA. 2003. NAD(P)H fluorescence imaging of postsynaptic neuronal activation in murine hippocampal slices. J Neurosci 23:3196-3208.
26. Sick TJ, Perez-Pinzon MA. 1999. Optical methods for probing mitochondrial function in brain slices. Methods 18:104-108.
27. Zhao YT, Tekkok S, Krnjevic K. 1997. 2-Deoxy-D-glucose-induced changes in membrane potential, input resistance, and excitatory postsynaptic potentials of CA1 hippocampal neurons. Can J Physiol Pharmacol 75:368-374.