Closed-tube DNA extraction using a thermostable proteinase is highly sensitive, capable of single parasite detection

Biotechnology Letters

Volumn 29, Issue 12, 2007, Pages 1831-1837

  Ferrari, B C ^a   Power, M L ^a   Bergquist, P L ^a,b  

^a MACQUARIE UNIVERSITY   (Australia)

^b UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

Cryptosporidium; DNA extraction; Giardia; Haemonchus; Nematoda; Proteinase; Protozoa

Indexed keywords

CONTAMINATION; ENZYMES; PROTOZOA; SENSITIVITY ANALYSIS;

CRYPTOSPORIDIUM; DNA EXTRACTION; NEMATODA; THERMOSTABLE PROTEINASE;

DNA;

PEPTIDE HYDROLASE; PROTOZOAL DNA;

AGAR GEL ELECTROPHORESIS; ANIMAL; ARTICLE; CATTLE; ENZYME STABILITY; FECES; FLOW CYTOMETRY; GENETIC PROCEDURES; GENETICS; GERBIL; HUMAN; IMMUNOMAGNETIC SEPARATION; ISOLATION AND PURIFICATION; METABOLISM; PARASITE; PARASITOLOGY; TEMPERATURE;

ANIMALS; CATTLE; DNA, PROTOZOAN; ELECTROPHORESIS, AGAR GEL; ENZYME STABILITY; FECES; FLOW CYTOMETRY; GENETIC TECHNIQUES; GERBILLINAE; HUMANS; IMMUNOMAGNETIC SEPARATION; PARASITES; PEPTIDE HYDROLASES; TEMPERATURE;

APICOMPLEXA; CRYPTOSPORIDIUM; GIARDIA; HAEMONCHUS; NEMATODA; PROTOZOA;

EID: 35748977394     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9487-2     Document Type: Article

References (24)

1. Anonymous (1999) Method 1623: Cryptosporidium and Giardia in water by filtration/IMS/FA. US Environment Protection Agency
2. Bennett JW, Gauci MR, Le Moenic S et al (1999) A comparison of enumeration techniques for Cryptosporidium parvum oocysts. J Parasitol 85:1165-1168
3. Ferrari BC, Vesey G, Davis KA et al (2000) A novel two-color flow cytometric assay for the detection of Cryptosporidium in environmental water samples. Cytometry 41:216-222
4. Hopkins RM, Meloni BP, Groth DM et al (1997) Ribosomal RNA sequencing reveals differences between genotypes of Giardia isolates recovered from humans and dogs living in the same locality. J Parasitol 83:44-51
5. Jiang J, Alderisio KA, Singh A et al (2005) Development of procedures for direct extraction of Cryptosporidium DNA from water concentrates and for relief of PCR inhibitors. Appl Environ Microbiol 71:1135-1141
6. Keister DB (1983) Axenic culture of Giardia lamblia in TYI-S-33 supplemented with bile. Trans Royal Soc Trop Med Hyg 77:487-488
7. Leoni F, Amar C, Nichols G et al (2006) Genetic analysis of Cryptosporidium from 2414 humans with diarrhoea in England between 1985 and 2000. J Med Microbiol 55:703-707
8. Limor JR, Lal AA, Xiao L (2002) Detection and differentiation of Cryptosporidium parasites that are pathogenic for humans by real-time PCR. J Clin Microbiol 40:2335-2338
9. MAFF (1971) Manual of veterinary parasitological techniques. Tech Bull 18:36-42
10. Moss D, Harbison SA, Saul DJ (2003) An easily automated, closed-tube forensic DNA extraction procedure using a thermostable proteinase. Int J Legal Med 117:340-349
11. Nantavisai K, Mungthin M, Tanariya P et al (2006) Evaluation of sensitivities of DNA extraction and PCR methods for detection of Giardia duodenalis in stool specimens. J Clin Microbiol 45:581-583
12. Ng CT, Gilchrist CA, Lane A et al (2005) Multiplex real-time PCR assay using scorpion probes and DNA capture for genotype-specific detection of Giardia lamblia on fecal samples. J Clin Microbiol 43:1256-1260
13. Nichols RAB, Moore JE, Smith HV (2006) A rapid method for extracting oocyst DNA from Cryptosporidium-positive human faeces for outbreak investigations. J Microbiol Meth 65:512-524
14. Orlandi PA, Lampel KA (2000) Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa. J Clin Microbiol 38:2271-2277
15. Power ML, Slade MB, Sangster NC et al (2004) Genetic characterisation of Cryptosporidium from a wild population of eastern grey kangaroos Macropus giganteus inhabiting a water catchment. Infect Gen Evol 4:59-67
16. Read CM, Monis PT, Thompson RC (2004) Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR-RFLP. Infect Gen Evol 4:125-130
17. Ryan U, Hawkins P, Swanson P et al (2005) Genotypes of Cryptosporidium from Sydney water catchment areas. J Appl Microbiol 98:1221-1229
18. Silvestre A, Humbert JF (2000) A molecular tool for species identification and benzimidazole resistance diagnosis in larval communities of small ruminant parasites. Exp Parasitol 95:271-276
19. Sluter SD, Tzipori S, Widmer G (1997) Parameters affecting polymerase chain reaction detection of waterborne Cryptosporidium parvum oocysts. Appl Environ Microbiol 48:325-330
20. Tiwari J, Kumar S, Kolte AP et al (2006) Detection of benzimidazole resistance in Haemonchus contortus using RFLP-PCR technique. Vet Parasitol 138:301-307
21. Truong Q, Ferrari BC (2006) Quantitative and qualitative comparisons of Cryptosporidium faecal purification procedures for the isolation of oocysts suitable for proteomic analysis. Int J Parasitol 36:811-819
22. Wilson IG (1997) Inhibition and facilitation of nucleic acid amplification. Appl Environ Microbiol 63:3741-3751
23. Xiao LH, Escalante L, Yang CF et al (1999) Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Appl Environ Microbiol 65:1578-1583
24. Zhu G, Marchewka MJ, Ennis JG et al (1998) Direct isolation of DNA from patient stools for polymerase chain reaction detection of Cryptosporidium parvum. J Infect Dis 177:1443-1446