Quantitative and qualitative comparisons of Cryptosporidium faecal purification procedures for the isolation of oocysts suitable for proteomic analysis

International Journal for Parasitology

Volumn 36, Issue 7, 2006, Pages 811-819

  Truong, Q ^a   Ferrari, B C ^a  

^a MACQUARIE UNIVERSITY   (Australia)

Author keywords

Cryptosporidium; Flow Cytometry; Oocysts; Proteomics; Purification; Sucrose Percoll

Indexed keywords

SUCROSE;

COMPARATIVE STUDY; FECES; FLOW CYTOMETRY; GENETIC ANALYSIS; NEONATE; PARASITE; PROTEIN; PROTOZOAN;

ANIMAL CELL; ARTICLE; BACTERIUM CONTAMINATION; CATTLE; CRYPTOSPORIDIUM; EPIFLUORESCENCE MICROSCOPY; FECES ANALYSIS; FLOW CYTOMETRY; GENOME; INTERMETHOD COMPARISON; ISOLATION PROCEDURE; NEWBORN; NONHUMAN; OOCYST; PROTEOMICS; PURIFICATION; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; SUCROSE GRADIENT; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANIMALS; CATTLE; CENTRIFUGATION, DENSITY GRADIENT; CRYPTOSPORIDIUM PARVUM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FECES; FLOW CYTOMETRY; MICROSCOPY, FLUORESCENCE; OOCYSTS; PROTEOMICS;

BACTERIA (MICROORGANISMS); CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM;

EID: 33744993639     PISSN: 00207519     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpara.2006.02.023     Document Type: Article

References (26)

1. Adam D.A. The biology of Giardia spp. Microbiological Reviews 55 (1991) 706-732
2. Arrowood M., and Sterling C. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. Journal of Parasitology 73 2 (1987) 314-319
3. Carucci D.J., Yates J.R., and Florens L. Exploring the proteome of Plasmodium. International Journal for Parasitology 32 (2002) 1539-1542
4. Chesnot T., and Schwartzbrod J. Quantitative and qualitative comparison of density based-based purification methods for detection of Cryptosporidium oocysts in turbid environmental matrices. Journal of Microbiological Methods 58 (2004) 375-386
5. Cohen A.M., Rumpel K., Coombs G., and Wastling J.M. Characterisation of global protein expression by two-dimensional electrophoresis and mass spectrometry: proteomics of Toxoplasma gondii. International Journal for Parasitology 32 (2002) 39-51
6. Current W.L. Techniques and Laboratory maintenance of Cryptosporidium. In: Dubay J.P., Speer C.A., and Fayer R. (Eds). Cryptosporidiosis of Man and Animals (1990), CRC Press, Boca Raton, FL
7. Davey H.M. Flow cytometric techniques for the detection of microorganisms. Methods in Cell Science 21 1-3 (2002) 91-97
8. Elliott D.A., and Clark D. Cryptosporidium parvum induces host cell actin accumulation at the host-parasite interface. Infection and Immunity 68 4 (2000) 2315-2322
9. Ferrari B.C., Vesey G., Weir C., Williams K.L., and Veal D.A. Comparison of Cryptosporidium-specific and Giardia-specific monoclonal antibodies for monitoring water samples. Water Research 33 7 (1999) 1611-1617
10. Gasser R.B., and O'Donoghue P. Isolation, propogation and characterisation of Cryptosporidium. International Journal for Parasitology 29 (1999) 1379-1413
11. Hijjawi N.S., Meloni B.P., Ng'anzo M., Ryan U.M., Olson M.E., Cox P.T., Monis P., and Thompson R.C.A. Complete development of Cryptosporidium parvum in host cell-free culture. International Journal for Parasitology 34 (2004) 769-777
12. Hunter P., and Gordon N. Epidemiology and clinical features of Cryptosporidium infection in immunocompromised patients. Clinical Microbiology Reviews 15 1 (2002) 145-154
13. Johnson J.R., Florens L., Carucci D.J., and Yates J.R. Proteomics in Malaria. Journal of Proteome Research 3 (2004) 296-306
14. Jungblut P. Proteome analysis of bacterial pathogens. Microbes and Infection 3 (2001) 831-840
15. Mackenzie W.R., Hoxie N.J., Proctor M.E., Gradus S., Blair K.A., Peterson D.E., Kazmierczak J.J., Addiss D.G., Fox K.R., Rose J.B., and Davis J.P. A massive outbreak in Milwaukee of Cryptosporidium infection transmitted through the public water supply. The New England Journal of Medicine 331 3 (1994) 161-167
16. Meisel J.L., Perera D.R., Meligro C., and Rubin C.E. Overwhelming watery diarrhoea associated with Cryptosporidium in an immunosuppressed patient. Gastroenterology 70 (1976) 1156-1160
17. Meloni B.P., and Thompson R.C.A. Simplified methods for obtaining purified oocysts from mice and for growing Cryptosporidium parvum in vitro. Journal of Parasitology 82 5 (1996) 757-762
18. Moss D.M., and Arrowood M.J. Quantification of Cryptosporidium parvum oocysts in mouse fecal specimens using immunomagnetic particles and two colour flow cytometry. Journal of Parasitology 87 2 (2001) 406-412
19. Pain A., Crossman L., Sebaihia M., Cerdeno-Tarraga A., and Parkhill J. Strength in diversity. Nature Reviews (2004) 358-359
20. Rossingnol J.F.A., Ayoub A., and Ayers M.S. Treatment of Diarrhea aused by Cryptosporidium parvum: a prospective randomised, double-blind, placebo-controlled study of Nitazoxanide. Journal of Infectious Diseases 184 (2001) 103-106
21. Sturbaum G.D., Jost H.B., and Sterling C. Nucleotide changes within three Cryptosporidium parvum surface protein encoding genes differentiate genotype I from genotype II isolates. Molecular and Biochemical Parasitology 128 (2003) 87-90
22. Tagh-Kilani R., and Sekla L. Purification of Cryptosporidium oocysts and sporozoites by caesium chloride and Percoll gradients. American Journal of Tropical Medicine and Hygiene 36 (1987) 505
23. Thompson R.C.A., and Chalmers R.M. Cryptosporidium: from molecules to disease. Trends in Parasitology 18 3 (2002) 98-100
24. Upton S.J. In vitro cultivation. In: Fayer R. (Ed). Cryptosporidium and Cryptosporidiosis (1997), CRC Press, Boca Raton, FL 181-207 (Chapter 8)
25. Winter G., Gooley A., Williams K., and Slade M. Characterisation of a major sporozoite surface glycoprotein of Cryptosporidium parvum. Functional and Integrative Genomics 1 3 (2000) 207-217
26. Xiao L., and Ryan U.M. Cryptosporidiosis: an update in molecular epidemiology. Current Opinion in Infectious Diseases 17 (2004) 483