Extraction-free, filter-based template preparation for rapid and sensitive PCR detection of pathogenic parasitic protozoa

Journal of Clinical Microbiology

Volumn 38, Issue 6, 2000, Pages 2271-2277

  Orlandi, Palmer A ^a   Lampel, Keith A ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CRYPTOSPORIDIUM PARVUM; CYCLOSPORA CAYETANENSIS; DNA TEMPLATE; ENCEPHALITOZOON INTESTINALIS; FOOD CONTAMINATION; MICROSPORIDA; NONHUMAN; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTOZOON; WATER CONTAMINATION;

BACTERIA (MICROORGANISMS); CRYPTOSPORIDIUM PARVUM; CYCLOSPORA CAYETANENSIS; ENCEPHALITOZOON INTESTINALIS; MICROSPORIDIA; PROTOZOA;

EID: 0034122543     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.38.6.2271-2277.2000     Document Type: Article

References (41)

1. Belgrader, P., S. A. Del Rio, K. A. Turner, M. A. Marino, K. R. Weaver, and P. E. Williams. 1995. Automated DNA purification and amplification from blood-stained cards using a robotic workstation. Biotechniques 19:426-432.
2. Bukhari, Z., and H. V. Smith. 1995. Effects of three concentration techniques on viability of Cryptosporidium parvum oocysts recovered from bovine feces. J. Clin. Microbiol. 33:2592-2595.
3. Burgoyne, L. A. March 1996. Solid medium and method for DNA storage. U.S. patent 5,496,562.
4. Centers for Disease Control. 1991. Outbreaks of diarrheal illness associated with cyanobacteria (blue-green algae)-like bodies - Chicago and Nepal, 1989 and 1990. Morbid. Mortal. Wkly. Rep. 40:325-327.
5. Centers for Disease Control. 1997. Outbreaks of Escherichia coli O175:H7 infection and cryptosporidiosis associated with drinking unpasteurized apple cider - Connecticut and New York, October 1996. Morbid. Mortal. Wkly. Rep. 46:4-8.
6. Centers for Disease Control. 1997. Update: outbreaks of cyclosporiasis - United States and Canada. Morbid. Mortal. Wkly. Rep. 46:521-523.
7. Centers for Disease Control. 1997. Outbreak of cyclosporiasis - Northern Virginia-Washington, D.C.-Baltimore, Maryland, metropolitan area, 1997. Morbid. Mortal. Wkly. Rep. 46:689-691.
8. Centers for Disease Control. 1998. Update: outbreak of cyclosporiasis - Ontario, Canada, May 1998. Morbid. Mortal. Wkly. Rep. 47:806-809.
9. Curry, A., and H. V. Smith. 1998. Emerging pathogens: Isospora, Cyclospora, and Microsporidia. Parasitology 117(Suppl):S143-S159.
10. da Silva, A. J., D. A. Schwartz, G. S. Visvesvara, H. De Moura, S. B. Slemenda, and N. J. Pieniazek. 1996. Sensitive PCR diagnosis of infections by Enterocytozoon bieneusi (Microsporidia) using primers based on the region coding for small-subunit rRNA. J. Clin. Microbiol. 34:986-987.
11. da Silva, A. J., S. B. Slemenda, G. S. Visvesvara, D. A. Schwartz, C. M. Wilcox, S. Wallace, and N. J. Pieniazek. 1997. Detection of Septata intestinalis (microsporidia) Cali et al. 1993 using polymerase chain reaction primers targeting the small subunit ribosomal RNA coding region. Mol. Diagn. 2:47-52.
12. da Silva, A. J., F. J. Bornay-Llinares, I. N. S. Moura, S. B. Slemenda, J. L. Tuttle, and N. J. Pieniazek. 1999. Fast and reliable extraction of protozoan parasite DNA from fecal specimens. Mol. Diagn. 4:57-64.
13. De Boer, S. H., L. J. Ward, X. Li, and S. Chittaranjan. 1995. Attenuation of PCR inhibition in the presence of plant compounds by addition of BLOTTO. Nucleic Acids Res. 23:2567-2568.
14. Del Aguila, C., R. Lopez-Velez, S. Fenoy, C. Turrientes, J. Cobo, R. Navajas, G. S. Visvesvara, G. P. Croppo, A. J. da Silva, and N. J. Pieniazek. 1997. Identification of Enterocytozoon bieneusi spores in respiratory samples from an AIDS patient with a 2-year history of intestinal microsporidiosis. J. Clin. Microbiol. 35:1862-1866.
15. Dowd, S. E., C. P. Gerba, and I. L. Pepper. 1998. Confirmation of the human-pathogenic microsporidia Enterocytozoon bieneusi, Encephalitozoon intestinalis, and Vittaforma comeae in water. Appl. Environ. Microbiol. 64: 3332-3335.
16. Fedorko, D. P., and Y. M. Hijazi. 1996. Application of molecular techniques to the diagnosis of microsporidial infection. Emerg. Infect. Dis. 2:183-190.
17. Flynn, P. M. 1996. Emerging diarrheal pathogens: Cryptosporidium parvum, Isospora belli, Cyclospora species, and Microsporidia. Pediatr. Ann. 25:480-481, 485-487.
18. Goodgame, R W. 1996. Understanding intestinal spore-forming protozoa: Cryptosporidia, Microsporidia, Isospora, and Cyclospora. Ann. Intern. Med. 124:429-441.
19. Hayes, E. B., T. D. Matte, T. R. O'Brien, T. W. McKinley, G. S. Logsdon, J. B. Rose, B. L. P. Ungar, D. M. Word, P. F. Pinskey, M. L. Cummings, M. A. Wilson, E. G. Long, E. S. Hurwitz, and D. D. Juranek. 1989. Large community outbreak of cryptosporidiosis due to contamination of a filtered public water supply. N. Engl. J. Med. 320:1372-1376.
20. Herwaldt, B. L., M.-L. Ackers, and the Cyclospora Working Group. 1997. An outbreak in 1996 of cyclosporiasis associated with imported raspberries. N. Engl. J. Med. 336:1548-1556.
21. Hoge, C. W., D. R. Shlim, M. Ghimire, et al. 1995. Placebo controlled trial of co-trimoxazole for Cyclospora infection among travelers and foreign residents in Nepal. Lancet 345:6991-6993.
22. Jinneman, K. C., J. H. Wetherington, W. E. Hill, A. M. Adams, J. M. Johnson, B. J. Tenge, N.-L. Dang, R. L. Manger, and M. M. Wekell. 1998. Template preparation for PCR and RFLP of amplification products for the detection and identification of Cyclospora sp. and Eimeria spp. oocysts directly from raspberries. J. Food Prot. 61:1497-1503.
23. Johnson, D. W., N. J. Pieniazek, D. W. Griffin, L. Misener, and J. B. Rose. 1995. Development of a PCR protocol for the sensitive detection of Cryptosporidium oocysts in water samples. Appl. Environ. Microbiol. 61:3849-3855.
24. Jones, C. S., P. P. M. Iannetta, M. Woodhead, H. V. Davies, R. J. McNicol, and M. A. Taylor. 1997. The isolation of RNA from raspberry (Rubus idaeus) fruit. Mol. Biotech. 8:219-221.
25. Laberge, I., M. W. Griffths, and M. W. Griffiths. 1996. Prevalence, detection, and control of Cryptosporidium parvum in food. Int. J. Food Microbiol. 32:1-26.
26. Lorenz, H., C. Jager, H. Willems, and G. Baljer. 1998. PCR detection of Coxiella burnetti from different clinical specimens, especially bovine milk, on the basis of DNA preparation with a silica matrix. Appl. Environ. Microbiol. 64:4234-4237.
27. MacKenzie, W. R., N. J. Hoxie, M. E. Proctor, M. S. Gradus, K. A. Blair, D. E. Peterson, J. J. Kazmierczak, D. S. Adiss, K. R. Fox, J. B. Rose, and J. P. Davis. 1994. A massive outbreak in Milwaukee of Cryptosporidium infection through the public water supply. N. Engl. J. Med. 331:161-167.
28. Manning, K. 1995. Isolation of nucleic acids from plants by differential solvent precipitation. Anal. Biochem. 195:45-50.
29. Millard, P. S., K. F. Gensheimer, D. G. Addiss, D. M. Sosin, G. A. Beckett, A. Houck-Jankoski, and A. Hudson. 1994. An outbreak of cryptosporidiosis from fresh-pressed apple cider. J. Am. Med. Assoc. 272:1592-1596.
30. Ortega, Y. R., C. R. Sterling, R. H. Gilman, V. A. Cama, and F. Diaz. 1993. Cyclospora species - a new protozoan pathogen of humans. N. Engl. J. Med. 328:1308-1312.
31. Ortega, Y. R., C. Roxas, R. Gilman, N. Miller, L. Cabera, C. Taquiri, and C. Sterling. 1997. Isolation of Cryptosporidium parvum and Cyclospora cayetanensis from vegetables collected in markets of an endemic region of Peru. Am. J. Trop. Med. Hyg. 57:683-686.
32. Rabold, J. G., C. W. Hoge, D. R. Shlim, C. Kefford, R. Rajah, and P. Echeverria. 1994. Cyclospora outbreak associated with chlorinated drinking water. Lancet 344:1360-1361.
33. Relman, D. A., T. M. Schmidt, A. Gajadhar, M. Sogin, J. Cross, K. Yoder, O. Sethabutr, and P. Echeverria. 1996. Molecular phylogenetic analysis of Cyclospora, the human intestinal pathogen, suggests that it is closely related to Eimeria species. J. Infect. Dis. 173:440-445.
34. Rinder, H., K. Janitschke, H. Aspock, A. J. da Silva, P. Deplazes, D. P. Fedorko, C. Franzen, U. Futh, F. Hunger, A. Lehmacher, C. G. Meyer, J. M. Molina, J. Sandfort, R. Weber, and T. Loscher. 1998. Blinded, externally controlled multicenter evaluation of light microscopy and PCR for the detection of microsporidia in stool specimens: the Diagnostic Multicenter Study Group on Microsporidia. J. Clin. Microbiol. 36:1814-1818.
35. Rogers, C., and L. Burgoyne. 1997. Bacterial typing: storage and processing of stabilized reference bacteria for polymerase chain reaction without preparing DNA - an example of an automatable procedure. Anal. Biochem. 247:223-227.
36. Sluter, S. D., S. Tzipori, and G. Widmer. 1997. Parameters affecting polymerase chain reaction detection of waterborne Cryptosporidium parvum oocysts. Appl. Microbiol. Biotechnol. 48:325-330.
37. Sturbaum, G. D., Y. R. Ortega, R. H. Gilman, C. R. Sterling, L. Cabrera, and D. A. Klein. 1998. Detection of Cyclospora cayetanensis in waste water. Appl. Environ. Microbiol. 64:2284-2286.
38. Visvesvara, G. S., G. J. Leitch, A. J. da Silva, G. P. Croppo, H. de Moura, S. Wallace, S. B. Slemenda, D. A. Schwartz, D. Moss, R. T. Bryan, and N. J. Pieniazek. 1994. Polyclonal and monoclonal antibody and PCR-amplified small-subunit rRNA identification of a microsporidian. Encephalitozoon hellem, isolated from an AIDS patient with disseminated infection. J. Clin. Microbiol. 32:2760-2768.
39. Visvesvara, G. S., A. J. da Silva, G. P. Croppo, N. J. Pieniazek, G. J. Leitch, D. Ferguson, H. de Moura, S. Wallace, S. B. Slemenda, I. Tyrrell, D. F. Moore, and J. Meador. 1995. In vitro culture and serologic and molecular identification of Septata intestinalis isolated from urine of a patient with AIDS. J. Clin. Microbiol. 33:930-936.
40. Wurtz, R. 1994. Cyclospora: a newly identified intestinal pathogen of humans. Clin. Infect. Dis. 18:620-623.
41. Yoder, K. E., O. Sethabutr, and D. A. Relman. 1996. PCR-based detection of the intestinal pathogen Cyclospora, p. 169-176. In D. H. Persing (ed.), PCR protocols for emerging infectious diseases, a supplement to diagnostic molecular microbiology: principles and applications. ASM Press, Washington, D.C.