Cell-specific effects of human cytomegalovirus unique region on recombinant protein expression

Biotechnology Letters

Volumn 29, Issue 12, 2007, Pages 1797-1802

  Lee, Jialing ^a   Lau, Joanne ^a   Chong, Grace ^a   Chao, Sheng Hao ^a  

^a AGENCY FOR SCIENCE   (Singapore)

Author keywords

CCAAT displacement protein; Cytomegalovirus major immediate early promoter; Pancreatic duodenal homeobox factor 1; Special AT rich sequence binding protein 1

Indexed keywords

CYTOMEGALOVIRUS; MAMMALIAN CELLS; TRANSGENE EXPRESSION;

BINDING SITES; CELLS; GENE EXPRESSION; GENETIC ENGINEERING; PROTEINS;

VIRUSES;

CELL EXTRACT; HOMEODOMAIN PROTEIN; INSULIN PROMOTER FACTOR 1; MATRIX ATTACHMENT REGION BINDING PROTEIN; RECOMBINANT PROTEIN; SATB1 PROTEIN, HUMAN; TRANSACTIVATOR PROTEIN; UNCLASSIFIED DRUG;

ANIMAL; ANTIBODY SPECIFICITY; ARTICLE; BIOLOGICAL MODEL; CELL NUCLEUS; CHO CELL; CRICETULUS; CYTOMEGALOVIRUS; GENETIC TRANSFECTION; GENETICS; HAMSTER; HELA CELL; HUMAN; METABOLISM; PLASMID; PROMOTER REGION;

ANIMALS; CELL EXTRACTS; CELL NUCLEUS; CHO CELLS; CRICETINAE; CRICETULUS; CYTOMEGALOVIRUS; HELA CELLS; HOMEODOMAIN PROTEINS; HUMANS; MATRIX ATTACHMENT REGION BINDING PROTEINS; MODELS, BIOLOGICAL; ORGAN SPECIFICITY; PLASMIDS; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; TRANS-ACTIVATORS; TRANSFECTION;

CYTOMEGALOVIRUS; HUMAN HERPESVIRUS 5; MAMMALIA;

EID: 35748974497     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9490-7     Document Type: Article

References (13)

1. Angulo A, Kerry D, Huang H, Borst EM, Razinsky A, Wu J, Hobom U, Messerle M, Ghazal P (2000) Identification of a boundary domain adjacent to the potent human cytomegalovirus enhancer that represses transcription of the divergent UL127 promoter. J Virol 74:2826-2839
2. Antes TJ, Namciu SJ, Fournier RE, Levy-Wilson B (2001) The 5′ boundary of the human apolipoprotein B chromatin domain in intestinal cells. Biochemistry 40:6731-6742
3. Banan M, Rojas IC, Lee WH, King HL, Harriss JV, Kobayashi R, Webb CF, Gottlieb PD (1997) Interaction of the nuclear matrix-associated region (MAR)-binding proteins, SATB1 and CDP/Cux, with a MAR element (L2a) in an upstream regulatory region of the mouse CD8a gene. J Biol Chem 272:18440-18452
4. Bode J, Benham C, Knopp A, Mielke C (2000) Transcriptional augmentation: modulation of gene expression by scaffold/matrix-attached regions (S/MAR elements). Crit Rev Eukaryot Gene Expr 10:73-90
5. Chao SH, Harada JN, Hyndman F, Gao X, Nelson CG, Chanda SK, Caldwell JS (2004) PDX1, a cellular homeoprotein, binds to and regulates the activity of human cytomegalovirus immediate early promoter. J Biol Chem 279:16111-16120
6. Girod PA, Zahn-Zabal M, Mermod N (2005) Use of the chicken lysozyme 5′ matrix attachment region to generate high producer CHO cell lines. Biotechnol Bioeng 91:1-11
7. Kohwi-Shigematsu T, Maass K, Bode J (1997) A thymocyte factor SATB1 suppresses transcription of stably integrated matrix-attachment region-linked reporter genes. Biochemistry 36:12005-12010
8. Lashmit PE, Lundquist CA, Meier JL, Stinski MF (2004) Cellular repressor inhibits human cytomegalovirus transcription from the UL127 promoter. J Virol 78:5113-5123
9. Lee J, Klase Z, Gao X, Caldwell JS, Stinski MF, Kashanchi F, Chao SH (2007) Cellular homeoproteins, SATB1 and CDP, bind to the unique region between the human cytomegalovirus UL127 and major immediate-early genes. Virology (in press)
10. Liu J, Bramblett D, Zhu Q, Lozano M, Kobayashi R, Ross SR, Dudley JP (1997) The matrix attachment region-binding protein SATB1 participates in negative regulation of tissue-specific gene expression. Mol Cell Biol 17:5275-5287
11. Lundquist CA, Meier JL, Stinski MF (1999) A strong negative transcriptional regulatory region between the human cytomegalovirus UL127 gene and the major immediate-early enhancer. J Virol 73:9039-9052
12. Meier JL, Stinski MF (1996) Regulation of human cytomegalovirus immediate-early gene expression. Intervirology 39:331-342
13. Namciu SJ, Blochlinger KB, Fournier RE (1998) Human matrix attachment regions insulate transgene expression from chromosomal position effects in Drosophila melanogaster. Mol Cell Biol 18:2382-2391