Regulation of human cytomegalovirus immediate-early gene expression

Intervirology

Volumn 39, Issue 5-6, 1996, Pages 331-342

  Meier, Jeffrey L ^a   Stinski, Mark F ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

21 bp repeats; Enhancer; Gfi I repressor; IE2 repressor; IEUS3 promoter; Major immediate early promoter; Modulator; NF 1

Indexed keywords

CELL PROTEIN; CIS ACTING ELEMENT; IMMEDIATE EARLY PROTEIN; MITOGENIC AGENT; PROTEIN KINASE; VIRUS PROTEIN;

ARTICLE; CELL DIFFERENTIATION; CELL TYPE; DNA TRANSFECTION; ENHANCER REGION; ENZYME ACTIVATION; GENE STRUCTURE; HUMAN; HUMAN CYTOMEGALOVIRUS; IMMEDIATE EARLY GENE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; REPRESSOR GENE; TRANSCRIPTION REGULATION; VIRION; VIRUS CELL INTERACTION; VIRUS GENE; VIRUS RECOMBINANT;

ANIMALS; ANTIGENS, VIRAL; CCAAT-ENHANCER-BINDING PROTEINS; CYTOMEGALOVIRUS; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, VIRAL; HUMANS; IMMEDIATE-EARLY PROTEINS; NFI TRANSCRIPTION FACTORS; TRANSCRIPTION FACTORS; Y-BOX-BINDING PROTEIN 1;

HUMAN HERPESVIRUS 5;

EID: 0030425424     PISSN: 03005526     EISSN: 14230100     Source Type: Journal    
DOI: 10.1159/000150504     Document Type: Article

References (114)

1. Zhou YF, Leon MB, Waclawiw MA, Popma JJ, Yu ZX, Finkcl T, Epstein SE: Association between prior cytomegalovirus infection and the risk of restenosis after coronary atherectomy, N Engl J Med 1996;335:624-630.
2. Onorato IM, Morens WJ, Stansfield SK: Epidemiology of cytomegalovirus infections: Recommendations for prevention and control, Rev Infect Dis 1985:7:479-497.
3. Fowler KB, Stagno S, Pass RF, Britt WJ, Boll TJ, Alford CA: The outcome of congenital cytomegalovirus infection in relation to maternal antibody status, N Engl J Med 1992:326:663- 667.
4. Gallant JE, Moore RD, Richman DD, Kcruly J, Chiasson RE, and the Zidovudine Epidemiology Study Group: Incidence and natural history of cytomegalovirus disease in patients with advanced human immunodeficiency virus disease treated with zidovudine, J Infect Dis 1992; 166:1223-1227.
5. Rubin RH: Impact of cytomegalovirus infection on organ transplant recipients, Rev Infect Dis 1990:12:S754-762.
6. Meyers J D, Flournoy N, Thomas ED: Nonbac-tcrial pneumonia after allogeneic marrow transplantation: A review of ten years' experience, Rev Infect Dis 1982:4:1119-1132.
7. Speir F., Modali R, Fluang E, Leon MB, Shawl F, Finkel T, Epstein SE: Potential role of human cytomegalovirus and p53 interaction in coronary restenosis, Science 1994:265:391 - 394.
8. Gratten MT, Moreno-Cabral CE, Starnes VA, Oyer PE, Stinson EB, Shumway NE: Cytomegalovirus infection is associated with cardiac allograft rejection and atherosclerosis, JAMA 1989;261:3561-3566.
9. Rubin RH: The indirect effects of cytomegalovirus infection on the outcome of organ transplantation, JAMA 1989:261:3607-3609.
10. Turtinen LW, Assimacopoulos A, Haase AT: Increased monokines in cytomegalovirus infected myelomonocytic cell cultures, Microb Pathog 1989;7:135-145.
11. Smith PD, Saini SS, Raffcld M, Manischcwitz JF, Wahl SM: Cytomegalovirus induction of tumor necrosis factor-a by human monocytes and mucosal macrophages, J Clin Invest 1992: 90:1642-1648.
12. Geist LJ, Monick MM, Stinski MF, Hunning-hake GW: The immediate early genes of human cytomegalovirus upregulate expression of the intcrleukin-2 and interleukin-2 receptor genes, Am J RespirCcll Mol Biol 1991:5:292-296.
13. Liu R, Baillie J, Sissons JG, Sinclair JH: The transcription factor YY1 binds to negative regulatory elements in the human cytomegalovirus major immediate early enhancer/promoter and mediates repression in non-pcrmissivc cells, Nucleic Acids Res 1994;22:2453-2459.
14. Sinzger C, Grefte A, Plachter B, Gouw ASH, Hauw The T, Jahn G: Fibroblasts, epithelial cells, endothelial cells, and smooth muscle cells are the major targets of human cytomegalovirus infection in lung and gastrointestinal tissues, J Gen Virol 1995;76:741-750.
15. Sinzger C, Plachter B, Grefte A, Hauw The T, Jahn G: Tissue macrophages are infected by human cytomegalovirus, J Infect Dis 1996; 173:240-245.
16. Schmidbauer M, Budka H, Ulrich W, Ambros P: Cytomegalovirus (CM V) disease of the brain in AIDS and connatal infection: Acomparative study by histology, immunocylochemistry, and in situ DNA hybridization, Acta Ncuropathol (Berlin) 1989:79:286-293.
17. Wiley CA, Schrier RD, Denaro FJ, Nelson JA, Lantpert PW, Oldstonc MB: Localization of cytomegalovirus proteins and genome during fulminant central nervous system infection in an AIDS patient, J Ncuropathol Exp Neurol 1986:45:127-139.
18. Gonczol E, Andrews PW, Plotkin SA: Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells, Science 1984;224:159-161.
19. Gonczol E, Andrews PW, Plotkin SA: Cytomegalovirus infection of human tcratocarcino-ma cells, J Gen Virol 1985:66:509-515.
20. LaFcmina R, Hayward GS: Constitutive and rctinoicacid-inducible expression of cytomegalovirus immediate-early genes in human terato-carcinoma cells, J Virol 1986;58:434-440.
21. Nelson JA, Groudine M: Transcriptional regulation of the human cytomegalovirus major immediate-early gene is associated with induction of DNase 1-hypersensitive sites, Mol Cell Biol 1986;6:452-461.
22. Ibanez CE, Schrier R, Ghazal P, Wiley C, Nelson JA: Human cytomegalovirus productively infects primary differentiated macrophages, J Virol 1991;65:6581-6588.
23. Kondo K, Kaneshima H, Mocarski ES: Human cytomegalovirus latent infection of granulo-cytc-macrophage progenitors, Proc Natl Acad Sei 1994:91:11879-11883.
24. Lathey JL, Spector SA: Unrestricted replication of human cytomegalovirus in hydrocortisone-treated macrophages, J Virol 1991;65: 6371-6375.
25. Macicjewski JP, Brucning EE, Donahue RE, Mocarski ES, Young NS, St Jcor JS: Infection of hematopoietic progenitor cells by human cytomegalovirus, Blood 1992:80:170- 178.
26. Minton EJ, Tysoe C, Sinclair JH, Sissons JG: Human cytomegalovirus infection of the monocyte/macrophage lineage in bone marrow, J Virol 1994;68:4017-4021.
27. Soderberg C, Larsson S, Bcrgstcdt-Lindqvist S, Möller E: Definition of a subset of human peripheral blood mononuclear cells that are permissive to human cytomegalovirus infection, J Virol 1993:67:3166-3175.
28. Tavlor-Wiedeman JA, Sissons JGP, Sinclair JH: Induction of endogenous human cytomegalovirus gene expression after differentiation of monocytes from healthy carriers, J Virol 1994: 68:1597-1604.
29. Turtinen LW, Seufzer BJ: Selective permissiveness of TP A differentiated TH P-1 myelomonocytic cells for human cytomegalovirus AD 169 and Towne, Microb Pathog 1994;16:373-378.
30. Weinshenker BG, Wilton S, Rice GPA: Phor-bol ester-induced differentiation permits productive human cytomegalovirus infection in a monocytic line, Immunology 1988; 140:1625- 1631.
31. Poland SD, Bambrick LL, Dckaban GA, Rice GPA: The extent of human cytomegalovirus replication in primary neurons is dependent on host cell differentiation, J Infect Dis 1994:170: 1267-1271.
32. Taylor-Wiedeman J, Sissons JG, Borysiewicz LK, Sinclair JH: Monocytes are a major site of persistence of human cytomegalovirus in peripheral blood mononuclear cells, J Gen Virol 1991;72:2059-2064.
33. Fish KN, Depto AS, Moses AV, Britt W, Nelson JA: Growth kinetics of human cytomegalovirus are altered in monocyte-derived macrophage, J Virol 1995;69:3737-3743.
34. Taylor-Wiedeman J, Hayhurst GP, Sissons JG, Sinclair JH: Polymorphonuclear cells are not sites of persistence of human cytomegalovirus in healthy individuals, J Gen Virol 1993:74: 265-268.
35. Waldman WJ, Knight DA, Huang EH, Scdntak DD: Bidirectional transmission of infectious cytomegalovirus between monocytes and vascular endothelial cells: An in vitro model, J Infect Dis 1995:171:263-272.
36. Wang Y, Stinski MF: Substitution of the human cytomegalovirus exon 5 of the IE2 gene with the bacterial guanine phosphoribosyl-transferasc gene (abstract 64), 21st Herpesvirus Workshop, Northern Illinois University, De-Kalb, July/Aug 1996.
37. Mocarski ES, Kemble G, Lyle J, Greaves RF: A deletion mutant in the human cytomegalovirus gene encoding 1E1 49laa is replication defective due to a failure in autoregulation, Proc Natl Acad Sci USA, in press.
38. Iskenderian AC, Huang L, Reilly A, Stcnbcrg RM, Anders DG: Four of eleven loci required for transient complementation of human cytomegalovirus DNA replication cooperate to activate expression of replication genes, J Virol 1996;70:383-392.
39. Cavanaugh V, Stenberg R, Staley T, Virgin HW, MacDonald MR, Pactzold S, Farrell HE, Rawlinson WD, Campbell AE: Murine cytomegalovirus with a deletion of genes spanning mdlll-J and -1 displays altered cell and tissue tropism, J Virol 1996;70:1365-1374.
40. Nelson J A, Reynolds-Kohler C, Smith B: Negative and positive regulation by a short segment in the 5-fianking region of the human cytomegalovirus major immediate-early gene, Mol Cell Biol 1987;7:4125-4129.
41. Huang TH, Oka T, Assai T, Okada T, Merrills BW, Gerston PN, Whitson RN, Itakura K: Repression by a differentiation-specific factor of the human cytomegalovirus enhancer, Nucleic Acids Res 1996:24:1695-1701.
42. Kothari SK, Baillie J, Sissons JGP, Sinclair JH: The 21 bp repeat element of the human cytomegalovirus major immediate early enhancer is a negative regulator of gene expression in undifferentiated cells, Nucleic Acids Res 1991: 19:1767-1771.
43. Shelbourn SL, Kothari SK, Sissons JGP, Sinclair JH: Repression of human cytomegalovirus gene expression associated with a novel immediate early regulatory region binding factor, Nucleic Acid Res 1989; 17:9165-9171.
44. Stein J, Volk H, Liebenthal C, Kruger DH, Prosch S: Tumor necrosis factor a stimulates the activity of the human cytomegalovirus major immediate early enhancer/promoter in immature monocytic cells, J Gen Virol 1993:74: 2333-2338.
45. Lubon H, Ghazal P, Henninghausen L, Rcy-nolds-Kohler C, Lockshin C, Nelson J: Cell-specific activity of the modulator region in the human cytomegalovirus major immediate-early gene, Mol Cell Biol 1989:9:1342-1345.
46. Thrower AR, Bullock GC, Bissell JE, Stinski MF: Regulation of a human cytomegalovirus immediate early gene (US3) by a silencer/ enhancer combination, J Virol 1996:70:91 - 100.
47. Sinclair JH, Baillie J, Bryant LA, Taylor-Wicd-emann JA, Sissons JG: Repression of human cytomegalovirus major immediate early gene expression in a monocytic cell line, J Gen Virol 1992;73:433-435.
48. Mendelson M, Sissons P, Sinclair J: Transcriptional regulation of the HCMV major immediate early promoter enhancer by Ets and Oct proteins (abstract 60), 21st Herpesvirus Workshop, Northern Illinois University, DeKalb, July/Aug 1996.
49. Meier JL, Stinski MF: Effect of modulator deletion on transcription of the human cytomegalovirus major immediate-early genes in infected undifferentiated and differentiated cells, J Virol, submitted.
50. Alccndor DJ, Gu W, Zong J, Waheed I, Peng RS, Chan YJ, Hayward GS: Conservation and divergence among control elements and binding sites in the major immediate-early enhancer of five primate cytomegaloviruses: Likely antagonistic role of the YYI repressor protein (abstract 70), 21st Herpesvirus Workshop, Northern Illinois University, DeKalb, July/ Aug 1996.
51. Hahn G, Mocarski E: Human cytomegalovirus latency and latently-infected cell types in hematopoietic progenitors and peripheral blood (abstract 303), 21st Herpesvirus Workshop, Northern Illinois University, DeKalb, July/ Aug 1996.
52. Zweidler-McKay PA, Grimes HL, Flubacher MM, Tsichlis PN: Gfi-1 encodes a nuclear zinc finger protein that binds DN A and functions as a transcriptional repressor, Mol Cell Biol 1996: 16:4024-4034.
53. Henninghausen L, Fleckenstein B: Nuclear factor I interacts with five DNA elements in the promoter region of the human cytomegalovirus major immediate early gene, EMBO J 1986:5: 1367-1371.
54. Jeang KT, Rawlins DR, Rosenfeld PJ, Shero JH, Kelly TJ, Hayward GS: Multiple tandcmly repealed binding sites for cellular nuclear factor I that surround the major immediate-early promoters of simian and human cytomegalovirus, J Virol 1987;61:1559-1570.
55. Stinski MF, Roehr TJ: Activation of the major immediate early gene of human cytomegalovirus by eis-acting elements in the promoter-regulatory sequence and by virus-specific transacting components, J Virol 1985;55:431-441.
56. Koedood R, Fichtel A, Meier P, Mitchell PJ: Human cytomegalovirus (HCMV) immediate-early enhancer/promoter specificity during em-bryogenesis defines target tissues of congenital HCMV infection, J Virol 1995:69:2194-2207.
57. Baskar JF, Smith PP, Nilauer G, Jupp RA, Hoffmann S, Pcffer NJ, Tenney DJ, Colbcrg-Poley AM, Ghazal P, Nelson JA: The enhancer domain of the human cytomegalovirus major immediate-early promoter determines cell type-specific expression in transgenic mice, J Virol 1996;70:3207-3214.
58. Baskar JF, Smith PP, Cimcnt GS, Hoffmann S, Tucker C, Tenney DJ, Colbcrg-Poley AM, Nelson JA, Ghazal P: Developmental analysis of the cytomegalovirus enhancer in transgenic animals, J Virol 1996;70:3215-3226.
59. Boshart M, Weber F, Jahn G, Dorsch-Hasler K, Fleckenstein B, Schaffner W: A very strong enhancer is located upstream of an immediate early gene of human cytomegalovirus, Cell 1985;41:521-530.
60. Stinski MF, Malone CL, Hermiston TW, Liu B: Regulation of human cytomegalovirus transcription: in Wagner EK (ed): Herpesvirus Transcription and Its Regulation, Boca Raton, CRC Press, 1991, pp 245-260.
61. Stinski MF, Macias MP, Malone CL, Thrower AR, Huang L: Regulation of transcription from the cytomegalovirus major immediate early promoter by cellular and viral proteins: in Mi-chelson S, Plotkin SA (eds): Multidisciplinary Approach to Understanding Cytomegalovirus Disease, Amsterdam, Elsevier, 1993, pp 3-12.
62. Thomsen DR, Stenberg RM, Goins WF, Stinski MF: Promoter-regulatory region of the major immediate early gene of human cytomegalovirus, Proc Natl Acad Sci USA 1984:81:659-663.
63. Dorsch-Hasler K, Keil GM, Weber F, Schaffner JM, Koszinowski UH: A long and complex enhancer activates transcription of the gene coding for the highly abundant immediate early mRNA in murine cytomegalovirus, Proc Natl Acad Sci USA 1985;82:8325-8329.
64. Chang YN, Crawford S, Stall J, Rawlins DR, Jeang KT, Hayward GS: The palindromic series I repeats in the simian cytomegalovirus major immediate-early promoter behave as both strong basal enhancers and cyclic-AMP response elements, J Virol 1990:64:264-277.
65. Alcendor DJ, Barry PA, Pratt-Lowe E, Luciw PA: Analysis of the rhesus cytomegalovirus immediate-early promoter, Virology 1993:194: 815-821.
66. Sandford GR, Bums WH: Rat cytomegalovirus has a unique immediate early gene enhancer, Virology 1996:222:310-317.
67. Hunninghake GW, Monick MM, Liu B, Stinski MF: The promoter-regulatory region of the major immediate-early gene of human cytomegalovirus responds to T-lymphocyte stimulation and contains functional cyclic-AMP response elements, J Virol 1989;63:3026-3033.
68. Fickcnscher H, Stammingcr T, Ruger R, Fleckenstein B: The role of a repetitive palindromic sequence clement in the human cytomegalovirus major immediate early enhancer, J Gen Virol 1989:70:107-123.
69. Niller HH, Henninghausen L: Phvtohcmagglu-tinin-induced activity of cyclic-AMP (cAMP) response elements from cytomegalovirus is reduced by cyclosporinc and svnergistically enhanced by cAMP, J Virol 1990:64:2388-2391.
70. Boldogh I, AbuBakar S, Albrecht T: Activation of proto-oncogenes: An immediate early event in human cytomegalovirus infection, Science 1990:247:561-564.
71. Cherrington JM, Mocarski ES: Human cytomegalovirus iel transactivates the a promoter enhancer via an 18-base pair repeat clement, J Virol 1989;63:1435-1440.
72. Sambucctti LC, Cherrington JM, Wilkinson GWG, Mocarski ES: NF-kappa B activation of the cytomegalovirus enhancer is mediated by a viral transactivator and bv T cell stimulation, EMBO J 1989;8:4251-4258.
73. Lacgreid A, Medvedev A, Nonstad U, Bombara MP, Ranges G, Sundan A, Espevik T: Tumor necrosis factor receptor p75 mediates cell-specific activation of nuclear factor kB and induction of human cytomegalovirus enhancer, J Biol Chem 1994;269:7785-7791.
74. Moch H, Lang D, Stamminger T: Strong transactivation of the human cytomegalovirus major immediate-early enhancer by p40lax of human T-cell leukemia virus type 1 via two repetitive tax-responsive sequence elements, J Virol 1992;66:7346-7354.
75. Ghazal P, MeDaltei C, Giulictti E, Kliewer SA, Umesono K: Retinoic acid receptors initiate induction of the cytomegalovirus enhancer in embryonal cells, Proc Natl Acad Sci USA 1992: 89:7630-7634.
76. Lang D, Fickcnscher H, Stammingcr T: Analysis of proteins binding to the proximal promoter region of the human cytomegalovirus IE-1/2 enhancer/promoter reveals both consensus and aberrant recognition sequences for transcription factors Sp-1 and CREB, Nucleic Acids Res 1992;20:3287-3295.
77. Shrivastava A, Caíame K: An analysis of genes regulated by the multi-functional transcription regulator Yin-Yang-1, Nucleic Acids Res 1994; 22:5151-5155.
78. Chang Y-N, Ycang K-T, Chiou C-J, Chan Y-J, Pizzorno M, Hayward GS: Identification of a large bent DNA domain and binding sites for serum response factor adjacent to the NFI repeat cluster and enhancer region in the major IE94 from simian cytomegalovirus, J Virol 1993;67:516-529.
79. Angulo A, Suto C, Boehm MF, Heyman RA, Ghazal P: Retinoid activation of rctinoic acid receptors but not of retinoid X receptors promotes ccllulardilTerentiationand replication of human cytomegalovirus in embryonal cells, J Virol 1995:69:3831-3837.
80. Angulo A, Suto C, Heyman RA, Ghazal P: Characterization of the sequences of the human cytomegalovirus enhancer that mediate differential regulation by natural and synthetic retinoids, Mol Endocrinol 1996;10:781-793.
81. Stenberg RM, Thomsen DR, Stinski MF: Structural analysis of the major immediate early gene of human cytomegalovirus, J Virol 1984:49:190-199.
82. Chang YN, Jcang KT, Lietman T, Hayward GS: Structural organization of the spliced immediate-early gene complex that encodes the major acidic nuclear (1E1) and transactivator (IE2) proteins of African green monkey cytomegalovirus, J BiomedSci 1995:2:105-130.
83. Javahery RA, Khachi A, Lo KZ-GB, Smalc ST: DNA sequence requirements for transcriptional initiator activity in mammalian cells, Mol Cell Biol 1994:14:116-127.
84. Macias MP, Huang L, Eashmit PE, Stinski MF: Cellular and viral protein binding to a cytomegalovirus promoter transcription initiation site: Effects on transcription, J Virol 1996:70:3628- 3635.
85. Hansen SK, Tjian R: TAFsand TFIIA mediate differential utilization of the tandem Adh promoters, Cell 1995:82:565-575.
86. Verrijzer CP, Chen J-L, Yokomori K, Tjian R: Binding of TAFs to core elements directs promoter selectivity by RNA polymerase II, Cell 1995:81:1115-1125.
87. Stenberg RM, Witte PR, Stinski MF: Multiple spliced and unspliced transcripts from human cytomegalovirus immediate-early region 2 and evidence for a common initiation site within immediate-early region 1, J Virol 1985:56: 665-675.
88. Liu B, Hcrmiston TW, Stinski MF: A m-acting element in the major immediate early (IE) promoter of human cytomegalovirus is required for negative regulation by IE2, J Virol 1991;65: 897-903.
89. Ghazal P, Nelson JA: Enhancement of RNA polymerase 11 initiation complexes by a novel DNA control domain downstream from the cap site of the cytomegalovirus major immediate-early promoter, J Virol 1991:65:2299-2307.
90. Chapman BS, Thayer RM, Vincent KA, Haig-wood NL: Effect of itttron A from human cytomegalovirus (Towne) immediate-early gene on heterologous expression in mammalian cells, Nucleic Acids Res 1991:19:3979-3986.
91. Radsak E, Fuhrmann R, Frankc RP, Schneider D, Kollcrt A, Briicher Kl I, Drenckhahn D: Induction by sodium butvratc of cytomegalovirus replication in human endothelial cells, Arch Virol 1989:107:151-158.
92. Tanaka J, Sadanari H, Sato H, Fukuda S: Sodium butyrate-inducible replication of human cytomegalovirus in a human epithelial cell line, Virology 1991:185:271-280.
93. Boldogh I, AbuBakar S, Deng CZ, Albrecht T: Transcriptional activation of cellular oncogenes fos.jun, and myc by human cytomegalovirus, J Virol 1991;65:1568-1571.
94. Liu B, Stinski MF: Human cytomegalovirus contains a tegument protein that enhances transcription from promoters with upstream ATF and AP-I cis-aeting elements, J Virol 1992:66:4434-4444.
95. Winkler M, Rice SA, Stamminger T: UL69 of human cytomegalovirus, an open reading frame with homology to ICP27 of herpes simplex virus, encodes a transactivator of gene expression, J Virol 1994;68:3943-3954.
96. Robertis DEM, Burglin TR, Fritz AT, Wright CVE, Jegalian B, Schnegelsberg P, Bittner D, Morita E, Oliver G, Cho YK: Families of vertebrate homcodomain proteins, UCLA Symp Ser 1989:95:107-115.
97. Wright CVE, Cho KWY, Oliver G, Robertis DEM: Vertebrate homeodomain proteins: Families of region-specific transcription factors, Trends Biochcm Sci 1989:14:52-56.
98. Michclson S, Turowski P, Picard L, Goris J, Landini MP, Topilko A, Hemmings B, Bessia C, Garcia A, Virelizier JL: Human cytomegalovirus carries serine/threonine protein phosphatases PP1 and a host-cell drived PP2A, J Virol 1996;70:1415-1423.
99. Chiou C-J, Zong J, Wahecd I, Hayward GS: Identification and mapping of dimerization and DNA-bindingdomains in the C terminus of the 1E2 regulatory protein of human cytomegalovirus, J Virol 1993:67:6201 -6214.
100. Chcrrington JM, Khoury EL, Mocarski ES: Human cytomegalovirus ie2 negatively regulation a gene expression via a short target sequence near the transcription start site, J Virol 1991;65:887-896.
101. Pizzorno MC, Hayward GS: The IE2 gene products of human cytomegalovirus specifically down-regulate expression from the major immediate-early promoter through a target located near the cap site, J Virol 1990:64: 6154-6165.
102. Lang D, Stamminger T: Minor groove contacts are essential for an interaction of the human cytomegalovirus IE2 protein with its DNA target, Nucleic Acids Res 1994:22: 3331-3338.
103. Macias MP, Stinski MF: Au in vitro system for human cytomegalovirus immediate early 2 protein (IE2)-mediated site-dependent repression of transcription and direct binding of IE2 to the major immediate early promoter, Proc Natl Acad Sci USA 1993:90:707-711.
104. Wu J, Jupp R, Stenberg RM, Nelson JA, Ghazal P: Site-specific inhibition of RNA polymerase II prcinitiation complex assembly by human cytomegalovirus IE86 protein, J Virol 1993:67:7547-7555.
105. Kaufman J, Sniale ST: Direct recognition of initiator elements by a component of the transcription factor IID complex, Genes Dev 1994:8:821-829.
106. Martinez E, Chiang C-M, Ga H, Roeder RG: TATA-binding protein-associated factor(s) in TF1ID function through the initiator to direct basal transcription from a TATA-less class II promoter, EMBO J 1994; 13:3115-3126.
107. Weston K: An enhancer element in the short unique region of human cytomegalovirus regulates the production of a group of abundant immediate early transcripts, Virology 1988; 162:406-416.
108. Chan Y-J, Tseng W-P, Hayward GS: Two distinct upstream regulators'domains containing multicopy cellular transcription factor binding sites provide basal repression and inducible enhancer characteristics to the immediate-early IES (US3) promoter from human cytomegalovirus, J Virol 1996:70:5312- 5328.
109. Biegalke BT: Regulation of human cytomegalovirus US3 gene transcription by a cis-repressive sequence, J Virol 1995:69:5362- 5367.
110. Biegalke BJ: Analysis of transcription repression of the human cytomegalovirus immediate early US3 gene (abstract 71), 21 st Herpesvirus Workshop, Northern Illinois University, DeKalb, July/Aug 1996.
111. Stamminger T, Puchtler E, Fleckcnstein B: Discordant expression of the immediate-early I ar.d 2 gene regions of human cytomegalovirus at early times after infection involves posltranscriptional processing events, J Virol 1991:65:2273-2282.
112. Tenney DJ, Colberg-Poley AM: Human cytomegalovirus UL36-38 and US3 immediate-early genes: Temporally regulated expression of nuclear cytoplasmic and polvsome-associ-ated transcripts during infection, J Virol 1991:65:6724-6734.
113. Zhao K, Hart CM, Laemmli UK: Visualization of chromosomal domains with boundary element-associated factor BEAF-32, Cell 1995;81:870-889.
114. Chec MA, Bankier AT, Beck S, Bohni R, Brown CM, Cerny R, Horsnell T, Hutchison CAI, Kouzarides T, Martignctti JA, Preddie E, Satchwell SC, Tomlinson P, Weston KM, Barrell BG: Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD 169, Curr Top Microbiol Immunol 1990:154:125-169.