Expression, purification and characterization of an iron-sulfur cluster assembly protein, IscU, from Acidithiobacillus ferrooxidans

Biotechnology Letters

Volumn 29, Issue 12, 2007, Pages 1965-1972

  Zeng, Jia ^a   Zhao, Wenjie ^a   Liu, Yuandong ^a   Xia, Lexian ^a   Liu, Jianshe ^a   Qiu, Guanzhou ^a  

^a CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

Acidithiobacillus ferrooxidans; Expression; His tag; Iron sulfur cluster; IscU; Purification

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; BACTERIA; ESCHERICHIA COLI; GENE EXPRESSION; MUTAGENESIS; SULFUR COMPOUNDS;

ACIDITHIOBACILLUS FERROOXIDANS; CLUSTER ASSEMBLY PROTEIN; CYSTEINE; IRON-SULFUR CLUSTER;

PROTEINS;

BACTERIAL PROTEIN; IRON SULFUR PROTEIN; MUTANT PROTEIN;

ACIDITHIOBACILLUS; AMINO ACID SEQUENCE; ARTICLE; BIOLOGICAL MODEL; CHEMISTRY; ELECTRON SPIN RESONANCE; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ALIGNMENT; ULTRAVIOLET SPECTROPHOTOMETRY;

ACIDITHIOBACILLUS; AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CLONING, MOLECULAR; CONSERVED SEQUENCE; ELECTRON SPIN RESONANCE SPECTROSCOPY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IRON-SULFUR PROTEINS; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; MUTANT PROTEINS; MUTATION; PLASMIDS; SEQUENCE ALIGNMENT; SPECTROPHOTOMETRY, ULTRAVIOLET;

ACIDITHIOBACILLUS FERROOXIDANS; ESCHERICHIA COLI;

EID: 35748948945     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9488-1     Document Type: Article

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